Rabbit Recombinant Monoclonal Met (c-Met) antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109673.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930)")
![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img138239.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930)")
![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img63771.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58550.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930)")
![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img38488.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (AB243930)")
Publications
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- Cancer research 82:4079-40922022MET-Induced CD73 Restrains STING-Mediated Immunogenicity of EGFR-Mutant Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyohei Yoshida,Maria Saigi,Tetsuo Tani,Benjamin F Springer,Hirofumi Shibata,Shunsuke Kitajima,Navin R Mahadevan,Marco Campisi,William Kim,Yoshihisa Kobayashi,Tran C Thai,Koji Haratani,Yurie Yamamoto,Shriram K Sundararaman,Erik H Knelson,Amir Vajdi,Israel Canadas,Ravindra Uppaluri,Cloud P Paweletz,Juan J Miret,Patrick H Lizotte,Prafulla C Gokhale,Pasi A Jänne,David A BarbiePubMed 36066413
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | Flow Cyt (Intra) | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Primary antibody incubation for 30 minutes at room temperature. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Primary antibody incubation for 30 minutes at 4°C. |
Associated Products
Select an associated product type
12 products for Alternative Product
3 products for Alternative Version
Target data
Function
Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (By similarity). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity). (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.
Alternative names
Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET
Recommended products
Rabbit Recombinant Monoclonal Met (c-Met) antibody. Carrier free. Suitable for IHC-P, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- SP44
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243930 is the carrier-free version of Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img109673.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Met (c-Met) with Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 at 1/50 dilution (4.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637)![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img138239.jpg "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)")
Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
Intracellular flow cytometric analysis ofHT-29 (human colorectal adenocarcinoma cell line) cell line labeling Met (c-Met) with Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637).
![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img63771.jpg "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)")
Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
This data was developed using the same antibody clone in a different buffer formulation (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637).
Flow cytometry overlay histogram showing wild-type HAP1 (green line) and MET knockout HAP1 cells stained with Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637) (1x106 in 100 µl at 0.04 µg/ml) for 30 min at 22°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/2000 for 30 min at 22°C.
Isotype control antibody was Rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line MET knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a .
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58550.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Met (c-Met) using Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 at 1/50 dilution in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637).
![Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930), expandable thumbnail](https://content.abcam.com/products/images/met-c-met-antibody-sp44-bsa-and-azide-free-ab243930--flow-cytometry-intracellular-img38488.jpg "Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)")
Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free (ab243930)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Met (c-Met) with purified Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637 at 1/20 dilution (11.3μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Met (c-Met) antibody [SP44] - C-terminal ab227637).
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