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Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637) is a rabbit monoclonal antibody that is used to detect Met (c-Met) in Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Specificity confirmed with Met (c-Met) knockout cell line validation


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (AB227637), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: 98% PBS, 1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PFlow Cyt (Intra)
Human
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Primary antibody incubation for 30 minutes at room temperature.

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/20 - 1/100
Notes

Primary antibody incubation for 30 minutes at 4°C.

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Target data

Function

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to hepatocyte growth factor/HGF ligand. Regulates many physiological processes including proliferation, scattering, morphogenesis and survival. Ligand binding at the cell surface induces autophosphorylation of MET on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1, SRC, GRB2, STAT3 or the adapter GAB1. Recruitment of these downstream effectors by MET leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. The RAS-ERK activation is associated with the morphogenetic effects while PI3K/AKT coordinates prosurvival effects. During embryonic development, MET signaling plays a role in gastrulation, development and migration of neuronal precursors, angiogenesis and kidney formation. During skeletal muscle development, it is crucial for the migration of muscle progenitor cells and for the proliferation of secondary myoblasts (By similarity). In adults, participates in wound healing as well as organ regeneration and tissue remodeling. Promotes also differentiation and proliferation of hematopoietic cells. May regulate cortical bone osteogenesis (By similarity). (Microbial infection) Acts as a receptor for Listeria monocytogenes internalin InlB, mediating entry of the pathogen into cells.

Alternative names

Hepatocyte growth factor receptor, HGF receptor, HGF/SF receptor, Proto-oncogene c-Met, Scatter factor receptor, Tyrosine-protein kinase Met, SF receptor, MET

Recommended products

Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637) is a rabbit monoclonal antibody that is used to detect Met (c-Met) in Flow Cytometry (Intra), Flow Cytometry, IHC-P. Suitable for Human samples.



- Specificity confirmed with Met (c-Met) knockout cell line validation

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
SP44
Purification technique
Affinity purification Protein A/G
Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637) is a rabbit recombinant monoclonal antibody and is validated for use in Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P) in Human samples.

Collaboration


Anti-Met (c-Met) [SP44] - C-terminal (ab227637) is a clone from the portfolio of Spring Bioscience (Roche) SP clones which have been optimised for immunohistochemistry (IHC).

Trusted by the scientific community


Anti-Met (c-Met) [SP44] - C-terminal (ab227637) was first used in a scientific publication in 2018 and has been cited over 10 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Specificity confirmed


The specificity of Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637) has been confirmed by Flow Cytometry testing in MET Knockout HAP1 cells.



Other related products


We have a range of other formats of antibody clone [SP44] also available for your convenience:
ab227637, Carrier free - Anti-Met (c-Met) antibody [SP44] - BSA and Azide free ab243930, APC - APC Anti-Met (c-Met) antibody [SP44] - C-terminal ab310844, PE - PE Anti-Met (c-Met) antibody [SP44] - C-terminal ab310915, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-Met (c-Met) antibody [SP44] - C-terminal ab311109, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Met (c-Met) antibody [SP44] - C-terminal ab313008, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Met (c-Met) - C-terminal antibody [SP44] ab314588, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Met (c-Met) antibody [SP44] - C-terminal ab321431



Product promise

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5 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon carcinoma tissue sections labeling Met (c-Met) with ab227637 at 1/50 dilution (4.52 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637)

    Flow cytometric analysis of HT-29 (Human colorectal adenocarcinoma cell line) cell line labeling Met (c-Met) with ab227637 at 1/100 dilution (green) compared with a negative control of rabbit IgG (blue).

  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637)

    Flow cytometry overlay histogram showing wild-type HAP1 (green line) and MET knockout HAP1 cells stained with ab227637 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab227637) (1x106 in 100 µl at 0.04 µg/ml) for 30 min at 22°C.

    The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) was used at 1/2000 for 30 min at 22°C.

    Isotype control antibody was Rabbit IgG (monoclonal) (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) used at the same concentration and conditions as the primary antibody (wild-type HAP1 - black line MET knockout HAP1 - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5000 events were collected using a .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637)

    Formalin-fixed, paraffin-embedded human colon adenocarcinoma tissue stained for Met (c-Met) using ab227637 at 1/50 dilution in immunohistochemical analysis.

  • Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Met (c-Met) antibody [SP44] - C-terminal (ab227637)

    Flow Cytometry analysis of HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling Met (c-Met) with purified ab227637 at 1/20 dilution (11.3 μg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / blue.

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Product protocols

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