AB5662

Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody

Name: Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662) | Abcam
Brand: Abcam Limited
SKU: AB5662
Price: 535 USD
Availability: InStock
Rating: 4 (7 reviews)

(7 Reviews)

(35 Publications)

Rabbit Polyclonal antibody. Suitable for WB and reacts with Human samples. Cited in 35 publications. Immunogen corresponding to Synthetic Peptide within Human MET phospho Y1234 + Y1235.

1 Images

Unknown

Western blot - Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (AB5662)

Peptide Competition :

Prior primary antibody incubation :

1 and 2 - no peptide,

3 - non-phosphopeptide corresponding to the immunogen,

4 -generic phosphotyrosine-containing peptide),

5 - phosphopeptide immunogen.

SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight at 4°C.

Bands were detected using the Pierce SuperSignal method.

The data show that only the phosphopeptide corresponding to c Met pYpYpY1230/1234/1235] block the antibody signal, demonstrating the specificity of the antibody.

Note : There are three isoforms of c Met, two of which are recognized by this antibody.

All lanes:

Western blot - Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (ab5662) at 1/1000 dilution

Lane 1:

Unstimulated (-), HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract

Lanes 2 - 5:

Stimulated (+) with HGF, HEK-293T whole cell extract

Secondary

All lanes:

Goat F(ab’)2 antirabbit IgG HRP conjugate

Predicted band size: 155 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human MET phospho Y1234 + Y1235. The exact immunogen used to generate this antibody is proprietary information.

P08581

Specificity

The phosphospecific antibody that has been generated does not distinguish between the dually (pYpY 1234/1235) and triply (pYpYpY1230/1234/1235) phosphorylated forms of c-Met, both of which are likely to represent activated forms of this receptor.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

Product details

Binding of scatter factor (SF)/hepatocyte growth factor (HGF) to the c Met receptor tyrosine kinase (RTK) triggers receptor dimerization and phosphorylation on multiple residues within the juxtamembrane, catalytic core and cytoplasmic tail domains, thereby regulating receptor internalization, catalytic activity and multisubstrate docking. c Met contains three tyrosines (Tyr-xx- x-Tyr-Tyr motif) within the activation loop of the catalytic domain. This is also seen with the insulin receptor, insulin-like growth factor receptor (IGF1) receptor and nerve growth factor (NGF) receptors/Trks, for which phosphorylation of all three tyrosines is required for full activation. With c Met (and the related family member, RON) phosphorylation of tyrosines 1234 and 1235 has been shown to be important in receptor activation. Activation of the c Met receptor results in binding and/or phosphorylation of many intracellular signaling proteins including multiple adaptor proteins (e.g., Grb2, Shc, Cbl, Crk, cortactin, paxillin, and GAB1), and a variety of other signal transducers (e.g., PI 3-kinase, FAK, Src, Erk1&2, JNK, PLC-ã, and STAT3).

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated c-Met protein. The final product is generated by affinity chromatography using a c Met-derived peptide that is phosphorylated at tyrosines 1230, 1234, 1235.

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

style

left-column

Publications (35)

Recent publications for all applications. Explore the full list and refine your search

Molecular therapy. Oncology 33:201032 PubMed40917509

2025

Aurora kinase A drives non-canonical YAP1/TAZ crosstalk to sustain primary resistance to anti-EGFR therapies in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Anxo Rio-Vilariño,Ana Garcia-Bautista,Aiora Cenigaonandia-Campillo,Pedro A Mateos-Gomez,Laura Garcia-Garcia,Marina I Schlaepfer,Laura Garcia-Hernandez,Laura Del Puerto-Nevado,Oscar Aguilera,Natalia Baños,Pablo Minguez,Víctor Manuel Castellano,Jesús Garcia-Foncillas,Arancha Cebrian

International journal of molecular sciences 25: PubMed39769478

2025

MET Exon 14 Skipping and Novel Actionable Variants: Diagnostic and Therapeutic Implications in Latin American Non-Small-Cell Lung Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Solange Rivas,Romina V Sepúlveda,Ignacio Tapia,Catalina Estay,Vicente Soto,Alejandro Blanco,Evelin González,Ricardo Armisen

Advances in pharmacological and pharmaceutical sciences 2024:2653426 PubMed39544485

2024

Saikosaponin-b2 Regulates the Proliferation and Apoptosis of Liver Cancer Cells by Targeting the MACC1/c-Met/Akt Signalling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yanxue Zhu,Xingzhi Lv,Ruifang Li,Zihan Gao,Chanhao Lei,Lan Wang,Sanqiang Li

iScience 27:110426 PubMed39108737

2024

A cyclic peptide-grafted Fc with hepatocyte growth factor functionality ameliorates hepatic fibrosis in a non-alcoholic steatohepatitis mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Nichole Marcela Rojas-Chaverra,Ryu Imamura,Hiroki Sato,Toby Passioura,Emiko Mihara,Tatsunori Nishimura,Junichi Takagi,Hiroaki Suga,Kunio Matsumoto,Katsuya Sakai

Biochemical pharmacology 195:114864 PubMed34861243

2021

Dictamnine, a novel c-Met inhibitor, suppresses the proliferation of lung cancer cells by downregulating the PI3K/AKT/mTOR and MAPK signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaojiao Yu,Lijing Zhang,Jun Peng,Richard Ward,Peiqi Hao,Jiwei Wang,Na Zhang,Yang Yang,Xiaoxi Guo,Cheng Xiang,Su An,Tian-Rui Xu

International journal of molecular sciences 22: PubMed34575841

2021

Novel Pyridine Bioisostere of Cabozantinib as a Potent -Met Kinase Inhibitor: Synthesis and Anti-Tumor Activity against Hepatocellular Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ujjwala Karmacharya,Diwakar Guragain,Prakash Chaudhary,Jun-Goo Jee,Jung-Ae Kim,Byeong-Seon Jeong

American journal of physiology. Gastrointestinal and liver physiology 321:G389-G399 PubMed34431407

2021

Circadian clock core component Bmal1 dictates cell cycle rhythm of proliferating hepatocytes during liver regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Huaizhou Jiang,Veronica Garcia,Jennifer Abla Yanum,Joonyong Lee,Guoli Dai

Experimental and therapeutic medicine 21:424 PubMed33747163

2021

Lidocaine suppresses the malignant behavior of gastric cancer cells via the c-Met/c-Src pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zeng,Ze Ting Xing,Mei Yun Tan,Yan Wen Wu,Chun Yuan Zhang

Science advances 6:eaay2801 PubMed32270033

2020

A chemically unmodified agonistic DNA with growth factor functionality for in vivo therapeutic application.

Applications

Unspecified application

Species

Unspecified reactive species

Ryosuke Ueki,Satoshi Uchida,Naoto Kanda,Naoki Yamada,Ayaka Ueki,Momoko Akiyama,Kazuko Toh,Horacio Cabral,Shinsuke Sando

American journal of physiology. Gastrointestinal a 318:G772-G780 PubMed32003603

2020

Pregnancy facilitates maternal liver regeneration after partial hepatectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Joonyong Lee,Veronica Garcia,Shashank Manohar Nambiar,Huaizhou Jiang,Guoli Dai

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody

Western blot - Anti-Met (c-Met) (phospho Y1230 + Y1234 + Y1235) antibody (AB5662)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (35)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise