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AB22620

Anti-Met Enkephalin antibody

3

(2 Reviews)

|

(5 Publications)

Rabbit Polyclonal Enkephalin antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PENK conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Proenkephalin-A, PENK

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met Enkephalin antibody (AB22620)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Met Enkephalin antibody (AB22620)

ab22620 (4µg/ml) staining met enkephalin in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining of seminal vesicles.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Immunocytochemistry/ Immunofluorescence - Anti-Met Enkephalin antibody (AB22620)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Met Enkephalin antibody (AB22620)

ICC/IF image of ab22620 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22620, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human PENK conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P01210

Specificity

Recognizes methionine enkephalin in a wide range of species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
Whole antiserum
Storage buffer
Preservative: 0.01% Sodium azide Constituents: Whole serum
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Met-enkephalin. Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.. Leu-enkephalin. Neuropeptide that competes with and mimic the effects of opiate drugs. They play a role in a number of physiologic functions, including pain perception and responses to stress.. Met-enkephalin-Arg-Phe. Met-enkephalin-Arg-Phe neuropeptide acts as a strong ligand of Mu-type opioid receptor OPRM1. Met-enkephalin-Arg-Phe-binding to OPRM1 in the nucleus accumbens of the brain increases activation of OPRM1, leading to long-term synaptic depression of glutamate release.. PENK(114-133). Increases glutamate release in the striatum and decreases GABA concentration in the striatum.. PENK(237-258). Increases glutamate release in the striatum.
See full target information PENK
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cognitive neurodynamics 18:2637-2658 PubMed39555258

2024

Involvement of prelimbic cortex neurons and related circuits in the acquisition of a cooperative learning by pairs of rats.

Applications

Unspecified application

Species

Unspecified reactive species

A R Conde-Moro,F Rocha-Almeida,E Gebara,J M Delgado-García,C Sandi,A Gruart

British journal of pharmacology 181:1268-1289 PubMed37990825

2023

Novel endomorphin analogues CEMR-1 and CEMR-2 produce potent and long-lasting antinociception with a favourable side effect profile at the spinal level.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Zhe Zhang,Si-Yu Wang,Xue-Ci Guo,Xiao-Han Liu,Xiao-Fang Wang,Meng-Meng Wang,Ting-Ting Qiu,Feng-Tong Han,Yao Zhang,Chang-Lin Wang

Nature communications 14:7167 PubMed37935684

2023

Proenkephalin-A secreted by renal proximal tubules functions as a brake in kidney regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Liu,Xiaoliang Liu,Zhongwei He,Jiangping Zhang,Xiaoqin Tan,Wenmin Yang,Yunfeng Zhang,Ting Yu,Shuyi Liao,Lu Dai,Zhi Xu,Furong Li,Yinghui Huang,Jinghong Zhao

The Journal of neuroscience : the official journal 40:5894-5907 PubMed32601247

2020

Mu-Opioids Suppress GABAergic Synaptic Transmission onto Orbitofrontal Cortex Pyramidal Neurons with Subregional Selectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Benjamin K Lau,Brittany P Ambrose,Catherine S Thomas,Min Qiao,Stephanie L Borgland

Proceedings of the National Academy of Sciences of 112:15486-91 PubMed26621716

2015

Mitochondrial function in the brain links anxiety with social subordination.

Applications

IHC-Fr

Species

Rat

Fiona Hollis,Michael A van der Kooij,Olivia Zanoletti,Laura Lozano,Carles Cantó,Carmen Sandi
View all publications
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