Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155]

16 product images

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  IHC image of Metabotropic Glutamate Receptor 3/MGLUR3 staining in a section of frozen normal human hippocampus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab281922, 1μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

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  Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  Negative control: liver (PMID: 9001707).

  The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

  Samples are non-boiled as boiling may cause protein aggregates.

  Exposure time: 3.25 seconds.

  All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

  Lane 1: Mouse brain tissue lysate at 20 µg

  Lane 2: Mouse liver tissue lysate at 40 µg

  Lane 3: Rat brain tissue lysate at 20 µg

  Lane 4: Rat liver tissue lysate at 40 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 100 kDa, 250 kDa

• 

  Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

  Samples are non-boiled as boiling may cause protein aggregates.

  Exposure time: 10 seconds.

  All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

  All lanes: Human brain tissue lysate at 20 µg

  Secondary

  All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

  Observed band size: 100 kDa, 250 kDa

• 

  Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  

  The molecular weight observed is consistent with what has been described in the literature (PMID: 27130562).

  Samples are non-boiled as boiling may cause protein aggregates.

  Exposure time: 1 seconds

  All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922) at 1/1000 dilution

  Lane 1: HEK-293T (human embryonic kidney) transfected with an empty vector, containing a myc-His-tag®, whole cell lysate at 20 µg

  Lane 2: HEK-293T transfected with Metabotropic glutamate receptor 3 expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 100 kDa, 250 kDa

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on mouse cerebellum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on human cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of paraffin-embedded Rat cerebrum tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Cytoplasmic staining on rat cerebrum. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labelling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/1000 dilution (0.342 ug/ml) followed by a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: No staining on mouse liver. The section was incubated with ab281922 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument. Counterstained with Hematoxylin.
  

  Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer Detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• 

  Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Cytoplasmic staining on mouse hippocampus is observed. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (3.42 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Cytoplasmic staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on rat liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

• 

  Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab281922 at 1/100 dilution (6.84 ug/ml) followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) at 1/1000 dilution (Green). Negative control: No staining on mouse liver (PMID: 9001707) is observed. The nuclear counterstain was DAPI (Blue).
  

  Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488)at 1/1000 dilution.

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  Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunofluorescent analysis of mouse primary neuron labeling Metabotropic Glutamate Receptor 3/MGLUR3. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton™ X-100. The cells were labeled with ab281922 at 1/50 dilution (6.84 μg/mL) and counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267) at 1/500 dilution (4 μg/mL). Then labeled with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (2 μg/mL) and counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 dilution (2 μg/mL). Nuclei were stained with DAPI. Confocal image showing cytoplasmic staining in mouse primary neuron.
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  

  Negative control 1: ab281922 1/50 (6.84 μg/mL), with secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 1/1000 (2 μg/mL)

  Negative control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 1/500 (4 μg/mL), with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 1/1000 (2 μg/mL)

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Immunofluorescent analysis of rat primary neuron labeling Metabotropic Glutamate Receptor 3/MGLUR3. The cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton™ X-100. The cells were labeled with ab281922 at 1/50 dilution (6.84 μg/mL) and counterstained with Anti-MAP2 mouse monoclonal antibody (Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267) at 1/500 dilution (4 μg/mL). Then labeled with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (2 μg/mL) and counterstained with Goat Anti-Mouse IgG H&L (Alexa Fluor® 594)/ (Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120) at 1/1000 dilution (2 μg/mL). Nuclei were stained with DAPI. Confocal image showing cytoplasmic staining in rat primary neuron.
  Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.
  

  Negative control 1: ab281922 1/50 (6.84 μg/mL), with secondary antibody Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120 1/1000 (2 μg/mL)

  Negative control 2: Anti-MAP2 antibody [HM-2] - Neuronal Marker ab11267 1/500 (4 μg/mL), with secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 1/1000 (2 μg/mL)

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  Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922)

  Blocking buffer: 5% NFDM /TBST.

  All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR24635-155] (ab281922) at 1/10000 dilution

  Lane 1: HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc/flag-tag, not boiled whole cell lysate at 20 µg

  Lane 2: HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR2 (aa1-872) with myc/flag-tag, not boiled whole cell lysate at 20 µg

  Lane 3: HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR3 (aa1-879) with myc/flag-tag, not boiled whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 95 kDa, 250 kDa

  Exposure time: 10s