Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975]

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1 : 1800 (0.36 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1 : 1800 (0.36 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1 : 1800 (0.36 μg/ml). Heat mediated antigen retrieval was performed using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) . Tissue was counterstained with Hematoxylin. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Flow Cytometry analysis of Mouse primary neuron cells cells labelling Metabotropic Glutamate Receptor 2/MGLUR2 with Purified ab150387 at 1 : 70 dilution (10 µg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Immunocytochemistry analysis of mouse primary neurone cells labelling MGLUR2 with ab150387 at 2 μg/mL. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150113) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-MAP2 rabbit monoclonal antibody (ab11267) at 1/500 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) at 1/1000 dilution (red). Nuclear DNA was labelled with DAPI (blue).

Confocal image showing positive staining in mouse primary neuron cells.

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection.

• WB

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution

Lane 1:

Mouse brain lysate boiled at 20 µg

Lane 2:

Mouse brain lysate unboiled at 20 µg

Lane 3:

Rat brain lysate boiled at 20 µg

Lane 4:

Rat brain lysate unboiled at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

• WB

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution

All lanes:

Human brain lysate unboiled

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

• WB

Lab

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (AB150387)

Blocking buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 2 + 3 / MGLUR2 + MGLUR3 antibody [EPR8975] (ab150387) at 1/10000 dilution

Lane 1:

HEK-293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc/flag-tag, not boiled whole cell lysate at 20 µg

Lane 2:

HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR2 (aa1-872) with myc/flag-tag, not boiled whole cell lysate at 20 µg

Lane 3:

HEK-293T (Human embryonic kidney epithelial cell) transfected with MGLUR3 (aa1-879) with myc/flag-tag, not boiled whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa,250 kDa

false

Exposure time: 3s