AB233637

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6]

Name: Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) | Abcam
Brand: Abcam Limited
SKU: AB233637
Price: 495 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal Metabotropic Glutamate Receptor 3/MGLUR3 antibody. Suitable for Flow Cyt, WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GRM3 aa 400 to C-terminus.

View Alternative Names

GPRC1C, MGLUR3, GRM3, Metabotropic glutamate receptor 3, mGluR3

5 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Paraffin-embedded human cervical cancer tissue stained for Metabotropic Glutamate Receptor 3/MGLUR3 using ab233637 at 1/200 dilution in immunohistochemical analysis. DAB staining.

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Flow cytometric analysis of SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling Metabotropic Glutamate Receptor 3/MGLUR3 with ab233637 at 1/200 dilution (green) compared with a negative control (red).

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for Metabotropic Glutamate Receptor 3/MGLUR3 (green) using ab233637 at 1/200 dilution in ICC/IF. Secondary antibody : Goat anti-Mouse IgG (H+L) DyLight 488.

Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Expected MW is 42.4 kDa.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution

All lanes:

Human Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576) recombinant protein

Predicted band size: 99 kDa

false

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Lanes 1 - 2:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (ab233637) at 1/500 dilution

Lanes 1 - 2:

Western blot - Anti-TIM 1 antibody [3D9F5] (<a href='/en-us/products/primary-antibodies/tim-1-antibody-3d9f5-ab233720'>ab233720</a>) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

Metabotropic Glutamate Receptor 3/MGLUR3 (aa 433-576)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 39 kDa,99 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7A5A6

Isotype

IgG2a

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

IHC-P, Flow Cyt, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRM3 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14832

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/1000", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from TCS.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

See full target information GRM3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of biochemical and molecular toxicology 34:e22436 PubMed31953908

2020

SENP1/HIF-1α axis works in angiogenesis of human dental pulp stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Zhou,Cong Sun

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [7A5A6] (AB233637)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

Product promise