Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized 293T (Human embryonic kidney epithelial cell) cells transfected with myc tagged human MGLUR3 construct labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1μg / Right), compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. A Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Cells were surface stained with rabbit IgG or ab283572. Then fixed with 2% PFA for 10 minutes followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor^® 647.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml, Green). Positive staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml, Green). The nuclear counterstain was DAPI (Blue). No staining on rat liver.

Negative control : liver (PMID : 9001707) is observed.

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml)

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of rat primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1μg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of mouse primary neuron cells labelling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/500 dilution (0.1μg / Right) compared with a Rabbit monoclonal IgG (ab172730 / Left) isotype control. Goat F(ab')2 Anti-Rabbit IgG(DyLight^® 488, ab98507) at 1/500 dilution was used as the secondary antibody. Gated on viable cells.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 μg/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml, Green). Positive staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml)

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labeling Metabotropic glutamate receptor 3/MGLUR3 with ab283572 at 1/100 dilution (5.85 ug/ml) followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/m, Green). The nuclear counterstain was DAPI (Blue). No staining on mouse liver.

Negative control : liver (PMID : 9001707) is observed.

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (2 μg/ml)

• IP

Supplier Data

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg rat brain tissue lysate with ab283572 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Rat brain tissue lysate 10μg

Lane 2 : ab283572 IP in Rat brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283572 in rat brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr24635-17-ab283572'>ab283572</a>)

Predicted band size: 99 kDa

Observed band size: 100 kDa,250 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Metabotropic glutamate receptor 3/MGLUR3 was immunoprecipitated from 0.35 mg mouse brain tissue lysate with ab283572 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab283572 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse brain tissue lysate 10μg

Lane 2 : ab283572 IP in Mouse brain tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab283572 in mouse brain tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 32 seconds

All lanes:

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr24635-17-ab283572'>ab283572</a>)

Predicted band size: 99 kDa

Observed band size: 100 kDa,250 kDa

false

• WB

Lab

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] - BSA and Azide free (AB283589)

This data was developed using ab283572, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Negative control : kidney, testis (PMID : 8840013).

The molecular weight observed is consistent with what has been described in the literature (PMID : 27130562).

Samples are non-boiled as boiling may cause protein aggregates.

Exposure time : 136 seconds

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR24635-17] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr24635-17-ab283572'>ab283572</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Rat brain tissue lysate at 20 µg

Lane 5:

Rat testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 99 kDa

Observed band size: 250 kDa

false