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AB249349

Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 3/MGLUR3 antibody. Carrier free. Suitable for WB, ICC/IF, Flow Cyt, I-ELISA and reacts with Rat, Mouse, Human, Recombinant fragment - Human samples.

View Alternative Names

GPRC1C, MGLUR3, GRM3, Metabotropic glutamate receptor 3, mGluR3

7 Images
Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • I-ELISA

Lab

Indirect ELISA - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

This data was developed using ab166608, the same antibody clone in a different buffer formulation.ELISA analysis of Human MGLUR3 recombinant protein at 500ng/ml with ab166608. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

This data was developed using ab166608, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Mouse primary neuron cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug)/ Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • Flow Cyt

Lab

Flow Cytometry - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

This data was developed using ab166608, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (Mouse neuroblastoma neuroblast) cells labelling Metabotropic Glutamate Receptor 3/MGLUR3 with ab166608 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • WB

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

This data was developed using ab166608, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr90092-ab166608'>ab166608</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human hypothalamus lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • WB

Unknown

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

This data was developed using ab166608, the same antibody clone in a different buffer formulation.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr90092-ab166608'>ab166608</a>) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results. This data was developed using ab166608, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr90092-ab166608'>ab166608</a>) at 1/1000 dilution

Lane 1:

Mouse brain lysate boiled at 20 µg

Lane 2:

Mouse brain lysate unboiled at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Exposure time: 3s

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)
  • WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] - BSA and Azide free (AB249349)

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration : 5% NFDM/TBST. We recommend not to boil the samples after lysis to get desired WB results. This data was developed using ab166608, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-3-mglur3-antibody-epr90092-ab166608'>ab166608</a>) at 1/1000 dilution

Lane 1:

Rat brain lysate boiled at 15 µg

Lane 2:

Rat brain lysate unboiled at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 99 kDa

Observed band size: 220 kDa

false

Exposure time: 7s

  • Unconjugated

    Anti-Metabotropic Glutamate Receptor 3/MGLUR3 antibody [EPR9009(2)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9009(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

I-ELISA, ICC/IF, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "guaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>" } } }
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Product details

ab249349 is the carrier-free version of ab166608.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.
See full target information GRM3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com