AB53088

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody

Name: Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088) | Abcam
Brand: Abcam Limited
SKU: AB53088
Price: 585 USD
Availability: InStock
Rating: 5 (18 reviews)

(18 Reviews)

(11 Publications)

Rabbit Polyclonal Metabotropic Glutamate Receptor 4/MGLUR4 antibody. Suitable for IHC-P, IHC-Fr, ELISA, WB, ICC/IF and reacts with Human, Cat, Mouse, Rat, Chicken samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human GRM4.

View Alternative Names

GPRC1D, MGLUR4, GRM4, Metabotropic glutamate receptor 4, mGluR4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

ICC/IF image of ab53088 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53088, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

ab53088 staining Human cerebellum. Staining is localised to the cell membrane.
Left panel : with primary antibody at 2 ug/ml. Right panel : isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the DAKO 3-in-1 antigen retrieval buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.

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Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (ab53088) at 1/500 dilution

Lane 1:

293 cell extracts, treated with Forskolin (40nM, 30mins)

Lane 2:

293 cell extracts, treated with Forskolin (40nM, 30mins) with peptide

Predicted band size: 102 kDa

Observed band size: 102 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Cat, Human, Mouse, Rat, Chicken

Applications

WB, ICC/IF, IHC-P, IHC-Fr, ELISA

applications

Immunogen

Synthetic Peptide within Human GRM4. The exact immunogen used to generate this antibody is proprietary information.

Q14833

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "2 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "5 µg/mL", "ICCIF-species-notes": "" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling inhibits adenylate cyclase activity.

See full target information GRM4

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed34982027

2022

Dissociation of impulsive traits by subthalamic metabotropic glutamate receptor 4.

Applications

Unspecified application

Species

Unspecified reactive species

Lukasz Piszczek,Andreea Constantinescu,Dominic Kargl,Jelena Lazovic,Anton Pekcec,Janet R Nicholson,Wulf Haubensak

JCI insight : PubMed33476302

2021

A GRM7 mutation associated with developmental delay reduces mGlu7 expression and produces neurological phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole M Fisher,Aqeela AlHashim,Aditi B Buch,Hana Badivuku,Manar M Samman,Kelly M Weiss,Gabriela I Cestero,Mark D Does,Jerri M Rook,Craig W Lindsley,P Jeffrey Conn,Rocco G Gogliotti,Colleen M Niswender

Journal of neurochemistry 156:465-480 PubMed32052426

2020

Activation of metabotropic glutamate receptor 4 regulates proliferation and neural differentiation in neural stem/progenitor cells of the rat subventricular zone and increases phosphatase and tensin homolog protein expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhichao Zhang,Xiaoyan Zheng,Yingfei Liu,Yan Luan,Li Wang,Lingyu Zhao,Jianshui Zhang,Yumei Tian,Haixia Lu,Xinlin Chen,Yong Liu

Bioscience, biotechnology, and biochemistry 84:279-289 PubMed31581881

2019

GRM4 inhibits the proliferation, migration, and invasion of human osteosarcoma cells through interaction with CBX4.

Applications

Unspecified application

Species

Unspecified reactive species

Zengliang Zhang,Nan Li,Xing Wei,Bingyao Chen,Yinglong Zhang,Yantao Zhao,Xiantong Hu,Shuxun Hou

BMC cancer 19:891 PubMed31492116

2019

Glutamate metabotropic receptor 4 (GRM4) inhibits cell proliferation, migration and invasion in breast cancer and is regulated by miR-328-3p and miR-370-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Bin Xiao,Daxiang Chen,Quan Zhou,Jianfeng Hang,Weiyun Zhang,Zhenzhan Kuang,Zhaohui Sun,Linhai Li

Cell and tissue research 378:471-483 PubMed31410629

2019

Expression patterns of L-amino acid receptors in the murine STC-1 enteroendocrine cell line.

Applications

Unspecified application

Species

Unspecified reactive species

Hongxia Wang,Karnam S Murthy,John R Grider

Scientific reports 8:5803 PubMed29643462

2018

Environment-dependent striatal gene expression in the BACHD rat model for Huntington disease.

Applications

Unspecified application

Species

Unspecified reactive species

Arianna Novati,Thomas Hentrich,Zinah Wassouf,Jonasz J Weber,Libo Yu-Taeger,Nicole Déglon,Huu Phuc Nguyen,Julia M Schulze-Hentrich

Neuropharmacology 108:462-73 PubMed26581500

2015

Functional modulation of G-protein coupled receptors during Parkinson disease-like neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Bruce G Jenkins,Aijun Zhu,Pekka Poutiainen,Ji-Kyung Choi,Kun-Eek Kil,Zhaoda Zhang,Darshini Kuruppu,Nurgul Aytan,Alpaslan Dedeoglu,Anna-Liisa Brownell

Journal of biomolecular screening 20:350-8 PubMed25394730

2014

Tetracycline-based system for controlled inducible expression of group III metabotropic glutamate receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Chruścicka,Grzegorz Burnat,Piotr Brański,Paulina Chorobik,Tomasz Lenda,Marcin Marciniak,Andrzej Pilc

British journal of pharmacology 166:2317-30 PubMed22404342

2012

Allosteric modulation of the group III mGlu4 receptor provides functional neuroprotection in the 6-hydroxydopamine rat model of Parkinson's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Matthew J Betts,Michael J O'Neill,Susan Duty

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody (AB53088)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (11)

Product promise