Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free

• IP

Supplier Data

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (AB250743)

This data was developed using ab184302, the same antibody clone in a different buffer formulation.

Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Mouse cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse cerebellum lysate, 10μg (Input).
Lane 2 : ab184302 IP in Mouse cerebellum lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A]-Isotype Control (ab172730) instead of ab184302 in Mouse cerebellum lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-4-mglur4-antibody-epr19280-ab184302'>ab184302</a>)

Predicted band size: 102 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (AB250743)

This data was developed using ab184302, the same antibody clone in a different buffer formulation.

Metabotropic Glutamate Receptor 4/MGLUR4 was immunoprecipitated from 1 mg of Rat cerebellum lysate with ab184302 at 1/40 dilution. Western blot was performed from the immunoprecipitate using ab184302 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Rat cerebellum lysate, 10μg (Input).
Lane 2 : ab184302 IP in Rat cerebellum lysate.
Lane 3 : Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab184302 in Rat cerebellum lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-4-mglur4-antibody-epr19280-ab184302'>ab184302</a>)

Predicted band size: 102 kDa

false

• WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (AB250743)

This data was developed using ab184302, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID : 8463825, 22528491), though results may vary depending on percentage acrylamide gel used.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-4-mglur4-antibody-epr19280-ab184302'>ab184302</a>) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 20 µg

Lane 2:

Human fetal liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution

Predicted band size: 102 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (AB250743)

This data was developed using ab184302, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID : 8463825, 22528491), though results may vary depending on percentage acrylamide gel used.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-4-mglur4-antibody-epr19280-ab184302'>ab184302</a>) at 1/1000 dilution

Lane 1:

Mouse cerebellum tissue lysate at 20 µg

Lane 2:

Rat cerebellum tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 102 kDa

Observed band size: ~300 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] - BSA and Azide free (AB250743)

This data was developed using ab184302, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This protein is specially expressed at cerebellum and the expression pattern is consistent with the literature (PMID : 8463825; 22528491), though results may vary depending on percentage acrylamide gel used.

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 4/MGLUR4 antibody [EPR19280] (<a href='/en-us/products/primary-antibodies/metabotropic-glutamate-receptor-4-mglur4-antibody-epr19280-ab184302'>ab184302</a>) at 1/1000 dilution

All lanes:

Mouse cerebellum lysate (2%SDS treated, not boiled) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 102 kDa

false

Exposure time: 3min