Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is a rabbit monoclonal antibody that is used to detect Metabotropic Glutamate Receptor 5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, EM. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
-Over 40 publications
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
IHC-P | IP | EM | WB | IHC-Fr | Flow Cyt (Intra) | |
---|---|---|---|---|---|---|
Human | Expected | Not recommended | Expected | Tested | Tested | Tested |
Mouse | Tested | Not recommended | Tested | Tested | Tested | Expected |
Rat | Tested | Not recommended | Expected | Expected | Expected | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/250 - 1/500 | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info 1/250 - 1/500 | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Mouse, Rat, Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Mouse | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/5000 - 1/10000 | Notes Please do not boil the sample before loading to the gel. |
Species Human | Dilution info 1/5000 - 1/10000 | Notes Please do not boil the sample before loading to the gel. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1 µg/mL | Notes This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
Species Mouse | Dilution info 1 µg/mL | Notes This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
Species | Dilution info | Notes |
---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/10 | Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
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G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5
Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is a rabbit monoclonal antibody that is used to detect Metabotropic Glutamate Receptor 5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, EM. Suitable for Human, Mouse, Rat samples.
- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency
-Over 40 publications
-Over 40 publications
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA
The Human species recommendation is based on the WB results. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).Positive staining on mouse cerebrum.
IHC image of Metabotropic Glutamate Receptor 5 staining in a section of frozen normal human hippocampus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab76316, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Buffer: 5% NFDM /TBST
Please do not boil the sample before loading to the gel.
GRM5 is a brain enriched protein, can be observed in most of the brain areas but low in spinal cord (PMID: 12783878).
All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution
Lane 1: Human spinal cord tissue lysate was not boiled before loading to the gel at 20 µg
Lane 2: Human hippocampus tissue lysate was not boiled before loading to the gel at 20 µg
Lane 3: Human liver tissue lysate was not boiled before loading to the gel at 20 µg
Lane 4: Human spinal cord tissue lysate was boiled before loading to the gel at 20 µg
Lane 5: Human hippocampus tissue lysate was boiled before loading to the gel at 20 µg
Lane 6: Human liver tissue lysate was boiled before loading to the gel at 20 µg
All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 132 kDa
Observed band size: 140 kDa, 280 kDa
All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution
All lanes: mouse brain lysate at 10 µg
All lanes: Goat anti-rabbit-HRP
Developed using the ECL technique.
Predicted band size: 132 kDa
Observed band size: 140-150 kDa, >250 kDa
Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat cerebrum.
ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.
Postembedding immunogold labeling of mouse neocortex using Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). The tissue was embedded in Lowicryl HM20 resin. 60 nm sections were then cut and mounted on nickel mesh grids before undergoing antigen retrieval for 15 minutes in 0.01 M citrate buffer, pH 6 at 60°C.
The sections were then blocked in 1% BSA/TBSN pH 7.6 and incubated overnight at room temperature with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1:250. Sections were washed twice in TBSN pH 7.6, treated with 1% normal donkey serum/TBSN pH 8.2 for 30 minutes, before being incubated with donkey anti-rabbit IgG-Au 10-20 nm at 1:20 for two hours at room temperature.
Sections were then washed in TBSN pH 8.2, followed by water, before undergoing post-staining with 1% uranyl acetate and Sato's lead. They were then air dried before being transferred to an oven for 30 minutes at 60°C.
In these images you can see gold immunoparticles on the postsynaptic density of synapses.
(TBSN = 0.02M TRIS buffered saline (0.3 N, pH 7.6 or 8.2) with 0.005% Tergitol NP-10)
Image collected and cropped by CiteAb under a CC-BY license from the publication
Metabotropic Glutamate Receptor 5 western blot using anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316. Publication image and figure legend from Chong, C. H., Li, Q., et al., 2019, Transl Psychiatry, PubMed 31582721.
ab76316 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76316 please see the product overview.
Daily intraperitoneal injection of CDPPB in juvenile mutant mice has transient beneficial effects.a A trend of reduced synaptic mGluR5 in mutants (one-way ANOVA, P = 0.1707, n indicated in graph). b Schematic diagram of the CDPPB treatment protocol. The whole litter of mice from heterozygous breeding pairs was kept with their mother throughout the experiment. c Homozygous mutant mice had significantly better weight gain after drug treatment (two-way repeated measure ANOVA, group: F(3,490) = 6.59, P = 0.0012; no significant difference between +/+ and +/m. P < 0.001 for all other pairwise comparison between two groups. Number of mice (n) indicated in graph. Data collected from seven litters. d Same mice showed a trend of reduced injury when counted at postnatal day 26 in CDPPB-treated litters (P = 0.236). +/+, wild-type; +/m, heterozygotes; m/m, homozygous Lrrc7 mutant
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