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Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is a rabbit monoclonal antibody that is used to detect Metabotropic Glutamate Receptor 5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, EM. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

-Over 40 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316), expandable thumbnail
  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316), expandable thumbnail
  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (AB76316), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPEMWBIHC-FrFlow Cyt (Intra)
Human
Expected
Not recommended
Expected
Tested
Tested
Tested
Mouse
Tested
Not recommended
Tested
Tested
Tested
Expected
Rat
Tested
Not recommended
Expected
Expected
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/250 - 1/500
Notes

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/250 - 1/500
Notes

Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000 - 1/10000
Notes

Please do not boil the sample before loading to the gel.

Species
Human
Dilution info
1/5000 - 1/10000
Notes

Please do not boil the sample before loading to the gel.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

Species
Mouse
Dilution info
1 µg/mL
Notes

This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

1 product for Alternative Version

Target data

Function

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.

Alternative names

Recommended products

Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is a rabbit monoclonal antibody that is used to detect Metabotropic Glutamate Receptor 5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, IHC-Fr, EM. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 40 publications

-Over 40 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR2425Y
Purification technique
Affinity purification Protein A
Specificity

The Human species recommendation is based on the WB results. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What is this antibody validated in?


Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Electron microscopy (EM), in Human, Mouse, Rat samples.

What is the molecular weight of Metabotropic Glutamate Receptor 5?


Anti-Metabotropic Glutamate Receptor 5 [EPR2425Y] (ab76316) specifically detects a band for Metabotropic Glutamate Receptor 5 (UniProt: P41594) at a molecular weight of 132kDa.

Recommended positive controls


WB: Mouse brain lysate and Human hippocampus tissue.IHC-P: Rat caudate putamen and cerebrum tissue; mouse caudate putamen tissue and cerebrumIHC-Fr: Mouse caudate ptamen tissue.IHC (resin): Mouse neocortex tissue.EM: Mouse neocortex tissue.Flow Cyt (intra): SH-SY5Y cells.

Trusted by the scientific community


Anti-Metabotropic Glutamate Receptor 5 [EPR2425Y] (ab76316) was first used in a scientific publication in 2009 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists


Anti-Metabotropic Glutamate Receptor 5 [EPR2425Y] (ab76316) has over 5 independent reviews from customers.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR2425Y] also available for your convenience:
ab76316, HRP - HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab196482, Carrier free - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free ab219374, Carrier free - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free ab256589, APC - APC Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab310880, PE - PE Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab310939, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab310960, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab311666, Alexa Fluor® 568 - Alexa Fluor® 568 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab312940, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab313151, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab321725



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).Positive staining on mouse cerebrum.

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    IHC image of Metabotropic Glutamate Receptor 5 staining in a section of frozen normal human hippocampus* performed on a Leica BOND™ system using the standard protocol. The section was fixed in 10% paraformaldehyde (10 min) prior to staining. The section was incubated with ab76316, 1 μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.


    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Buffer: 5% NFDM /TBST

    Please do not boil the sample before loading to the gel.

    GRM5 is a brain enriched protein, can be observed in most of the brain areas but low in spinal cord (PMID: 12783878).

    All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution

    Lane 1: Human spinal cord tissue lysate was not boiled before loading to the gel at 20 µg

    Lane 2: Human hippocampus tissue lysate was not boiled before loading to the gel at 20 µg

    Lane 3: Human liver tissue lysate was not boiled before loading to the gel at 20 µg

    Lane 4: Human spinal cord tissue lysate was boiled before loading to the gel at 20 µg

    Lane 5: Human hippocampus tissue lysate was boiled before loading to the gel at 20 µg

    Lane 6: Human liver tissue lysate was boiled before loading to the gel at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 132 kDa

    Observed band size: 140 kDa, 280 kDa

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1/10000 dilution

    All lanes: mouse brain lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit-HRP

    Developed using the ECL technique.

    Predicted band size: 132 kDa

    Observed band size: 140-150 kDa, >250 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).

    Positive staining on rat cerebrum.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail
    This image is courtesy of a customer review submitted by Carl Hobbs.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.

  • Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.

  • Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail
    Images courtesy of Professor Richard Weinberg, UNC School of Medicine

    Electron Microscopy - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Postembedding immunogold labeling of mouse neocortex using Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316). The tissue was embedded in Lowicryl HM20 resin. 60 nm sections were then cut and mounted on nickel mesh grids before undergoing antigen retrieval for 15 minutes in 0.01 M citrate buffer, pH 6 at 60°C.

    The sections were then blocked in 1% BSA/TBSN pH 7.6 and incubated overnight at room temperature with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316) at 1:250. Sections were washed twice in TBSN pH 7.6, treated with 1% normal donkey serum/TBSN pH 8.2 for 30 minutes, before being incubated with donkey anti-rabbit IgG-Au 10-20 nm at 1:20 for two hours at room temperature.

    Sections were then washed in TBSN pH 8.2, followed by water, before undergoing post-staining with 1% uranyl acetate and Sato's lead. They were then air dried before being transferred to an oven for 30 minutes at 60°C.

    In these images you can see gold immunoparticles on the postsynaptic density of synapses.

    (TBSN = 0.02M TRIS buffered saline (0.3 N, pH 7.6 or 8.2) with 0.005% Tergitol NP-10)

  • Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] (ab76316)

    Metabotropic Glutamate Receptor 5 western blot using anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316. Publication image and figure legend from Chong, C. H., Li, Q., et al., 2019, Transl Psychiatry, PubMed 31582721.


    ab76316 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab76316 please see the product overview.

    Daily intraperitoneal injection of CDPPB in juvenile mutant mice has transient beneficial effects.a A trend of reduced synaptic mGluR5 in mutants (one-way ANOVA, P = 0.1707, n indicated in graph). b Schematic diagram of the CDPPB treatment protocol. The whole litter of mice from heterozygous breeding pairs was kept with their mother throughout the experiment. c Homozygous mutant mice had significantly better weight gain after drug treatment (two-way repeated measure ANOVA, group: F(3,490) = 6.59, P = 0.0012; no significant difference between +/+ and +/m. P < 0.001 for all other pairwise comparison between two groups. Number of mice (n) indicated in graph. Data collected from seven litters. d Same mice showed a trend of reduced injury when counted at postnatal day 26 in CDPPB-treated litters (P = 0.236). +/+, wild-type; +/m, heterozygotes; m/m, homozygous Lrrc7 mutant

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