Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free
- RabMAb
- Recombinant
- What is this?
Be the first to review this product! Submit a review
|
(5 Publications)
Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra), EM, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
View Alternative Names
GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using ab76316 (red) at 1/20 or a rabbit IgG (ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.
- IHC-P
Collaborator
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316)
ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.
This image is courtesy of an Abreview submitted by Carl Hobbs.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316)
Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat cerebrum.
- IHC-P
Lab
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76316).
Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on mouse cerebrum.
Related conjugates and formulations (10)
-
519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
HRP Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - BSA and Azide free
-
660 APC
APC Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
578 PE
PE Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
-
775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y]
Reactivity data
Product details
ab219374 is the carrier-free version of ab76316.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (5)
Recent publications for all applications. Explore the full list and refine your search
Molecular psychiatry 20:1546-56 PubMed26370144
2015
Applications
Unspecified application
Species
Unspecified reactive species
Molecular psychiatry 20:959-73 PubMed25266126
2014
Applications
Unspecified application
Species
Unspecified reactive species
Physiological reports 2:e00260 PubMed24760514
2014
Applications
WB
Species
Mouse
The Journal of neuroscience : the official journal of the Society for Neuroscience 32:16391-401 PubMed23152621
2012
Applications
WB
Species
Unspecified reactive species
Cerebral cortex (New York, N.Y. : 1991) 23:2179-89 PubMed22791805
2012
Applications
ICC/IF, WB
Species
Mouse, Mouse
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com