Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra), EM, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184633.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174096.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136614.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)")
![Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--flow-cytometry-intracellular-img50457.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (AB219374)")
Publications
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- Molecular psychiatry 20:1546-562015Alteration by p11 of mGluR5 localization regulates depression-like behaviors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesK-W Lee et. al.PubMed 26370144
- Molecular psychiatry 20:959-732014Neuregulin 1 signalling modulates mGluR1 function in mesencephalic dopaminergic neurons.Applications:Unspecified applicationReactive species:Unspecified reactive speciesA Ledonne et. al.PubMed 25266126
- Physiological reports 2:e002602014The mouse cyclophosphamide model of bladder pain syndrome: tissue characterization, immune profiling, and relationship to metabotropic glutamate receptors.
- The Journal of neuroscience : the official journal of the Society for Neuroscience 32:16391-4012012PKC phosphorylation regulates mGluR5 trafficking by enhancing binding of Siah-1A.Applications:WBReactive species:Unspecified reactive speciesSuk Jin Ko,Kaname Isozaki,Insook Kim,Jeong Ho Lee,Ho Jin Cho,Sun Young Sohn,So Ra Oh,Steven Park,Dong Goo Kim,Chul Hoon Kim,Katherine W RochePubMed 23152621
- Cerebral cortex (New York, N.Y. : 1991) 23:2179-892012Compensatory molecular and functional mechanisms in nervous system of the Grm1(crv4) mouse lacking the mGlu1 receptor: a model for motor coordination deficits.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | Flow Cyt (Intra) | EM | IHC-Fr | |
|---|---|---|---|---|---|---|
| Human | Expected | Not recommended | Tested | Tested | Expected | Expected |
| Mouse | Tested | Not recommended | Tested | Expected | Tested | Tested |
| Rat | Tested | Not recommended | Expected | Expected | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Use of HRP-conjugated or polymerized HRP secondary antibodies, stronger signals have been found using the polymerized HRP secondary. We only recommend mouse and rat species for IHC-P. We do not guarantee IHC for Human species. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Please do not boil the sample before loading to the gel. |
Species Human | Dilution info - | Notes Please do not boil the sample before loading to the gel. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376- Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
EM
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-Fr
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Species Human | Dilution info 1 µg/mL | Notes This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples. |
Associated Products
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7 products for Alternative Version
Target data
Function
G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
Alternative names
GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5
Recommended products
Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody. Carrier free. Suitable for IHC-P, WB, Flow Cyt (Intra), EM, IHC-Fr and reacts with Mouse, Rat, Human samples. Cited in 5 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR2425Y
- Purification technique
- Affinity purification Protein A
- Specificity
The Human species recommendation is based on the WB results. This antibody has been tested for IHC-P and IHC-Fr in Human samples and we obtain positive signal only in IHC-Fr. We do not recommend this antibody for IHC-P in Human samples.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab219374 is the carrier-free version of Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
What does low endotoxin mean?
Our low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img184633.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316)
Immunohistochemical analysis of Paraffin-embedded rat cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
Positive staining on rat cerebrum.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img174096.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316)Immunohistochemical analysis of Paraffin-embedded mouse cerebrum tissue sections labeling Metabotropic Glutamate Receptor 5 with Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316 at 1/400 dilution followed by Goat Anti-Rabbit IgG H&L (HRP Polymer) secondary antibody. Sections were counterstained with Hematoxylin. Antigen retrieval was heat mediated using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).Positive staining on mouse cerebrum.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img136614.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)")
This image is courtesy of a customer review submitted by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316)
Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316 staining Metabotropic Glutmate Receptor 5 in mouse caudate putamen/ Corpus callosum by immunohistochemistry. Tissue was fixed with formaldehyde and citrate-mediated antigen retrieval was performed. Samples were blocked with 1% BSA for 10 minutes at 21°C, before incubation with the primary antibody (1/1000) for 2 hours at 21°C. A biotin conjugated goatanti-rabbit IgG secondary was used at 1/250.
![Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374), expandable thumbnail](https://content.abcam.com/products/images/metabotropic-glutamate-receptor-5-antibody-epr2425y-low-endotoxin-azide-free-ab219374--flow-cytometry-intracellular-img50457.jpg "Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)")
Flow Cytometry (Intracellular) - Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] - Low endotoxin, Azide free (ab219374)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316).
Intracellular flow cytometric analysis of permeabilized SH-SY5Y cells using Anti-Metabotropic Glutamate Receptor 5 antibody [EPR2425Y] ab76316 (red) at 1/20 or a rabbit IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) as a negative control (green). The cells were permeabilized with 2% PFA and a goat anti-rabbit IgG FITC was used as the secondary at 1/150.
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