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Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 7/MGLUR7 antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

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Images

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530), expandable thumbnail
  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PIHC-FrICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Expected
Not recommended
Not recommended
Expected
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended
Tested
Rat
Tested
Tested
Tested
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Rat
Dilution info
1/50
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Function

G-protein coupled receptor activated by glutamate that regulates axon outgrowth through the MAPK-cAMP-PKA signaling pathway during neuronal development (PubMed:33500274). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase that it inhibits (PubMed:9473604).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 7/MGLUR7 antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR24530-11
Purification technique
Affinity purification Protein A
Specificity

This antibody is unsuitable for human IHC-P and mouse IHC-FR. 

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

That antibody is unsuitable for human IHC-P and mouse IHC-FR. Also, ab302530 does not react in ICC and intracellular flow cytometry respectively with human and mouse+rat species.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID: 33767338, 19109906).

    All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution

    All lanes: SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 260 kDa

    Exposure time: 92s

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/50 (10.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control: no staining on rat heart (PMID 12052533). is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/50 (10.02 ug/ml) dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue).
    Secondary antibody control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.
    .

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on rat cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining on mouse cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of paraffin-embedded (A) HEK-293T ((human tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/500 (1ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on (A) HEK-293T transfected with a Myc-His tagged GRM7 construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab302530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on rat hippocampus (PMID: 11007882, PMID: 9295396). The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on mouse hippocampus (PMID: 11007882, PMID: 9295396). The section was incubated with ab302530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

  • Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530), expandable thumbnail

    Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Negative controls: heart, skeletal muscle, spleen (PMID: 12052533).
    260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID: 33767338, 19109906).

    All lanes: Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution

    Lane 1: Mouse hippocampus tissue lysate 20 µg

    Lane 2: Mouse spinal cord tissue lysate 20 µg

    Lane 3: Mouse heart tissue lysate 20 µg

    Lane 4: Mouse skeletal muscle tissue lysate 20 µg

    Lane 5: Mouse spleen tissue lysate 20 µg

    Lane 6: Rat hippocampus tissue lysate 20 µg

    Lane 7: Rat heart tissue lysate 20 µg

    Lane 8: Rat skeletal muscle tissue lysate 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 260 kDa, 258 kDa

    Exposure time: 81s

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Product protocols

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