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AB302530

Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 7/MGLUR7 antibody. Suitable for WB, IHC-P, IHC-Fr, IP and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

GPRC1G, MGLUR7, GRM7, Metabotropic glutamate receptor 7, mGluR7

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T ((human tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/500 (1ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on (A) HEK-293T transfected with a Myc-His tagged GRM7 construct, no staining on (B) HEK-293T transfected with empty plasmid. The section was incubated with ab302530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on mouse cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat heart (fresh) tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/50 (10.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Negative control : no staining on rat heart (PMID 12052533). is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of paraffin-embedded Rat hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on rat hippocampus (PMID : 11007882, PMID : 9295396). The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of paraffin-embedded Mouse hippocampus tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse hippocampus (PMID : 11007882, PMID : 9295396). The section was incubated with ab302530 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh) tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/50 (10.02 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green). Positive staining on rat hippocampus is observed. The nuclear counterstain was DAPI (Blue). Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution. .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Immunohistochemical analysis of paraffin-embedded Rat cardiac muscle tissue labeling Metabotropic Glutamate Receptor 7/MGLUR7 with ab302530 at 1/1000 (0.5ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining on rat cardiac muscle. The section was incubated with ab302530 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID : 33767338, 19109906).

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution

All lanes:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa

true

Exposure time: 92s

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)
  • WB

Supplier Data

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (AB302530)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative controls : heart, skeletal muscle, spleen (PMID : 12052533). 260-kDa GRM7 dimer is observed. The molecular weight is consistent with literature (PMID : 33767338, 19109906).

All lanes:

Western blot - Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (ab302530) at 1/1000 dilution

Lane 1:

Mouse hippocampus tissue lysate 20 µg

Lane 2:

Mouse spinal cord tissue lysate 20 µg

Lane 3:

Mouse heart tissue lysate 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate 20 µg

Lane 5:

Mouse spleen tissue lysate 20 µg

Lane 6:

Rat hippocampus tissue lysate 20 µg

Lane 7:

Rat heart tissue lysate 20 µg

Lane 8:

Rat skeletal muscle tissue lysate 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 260 kDa,258 kDa

false

Exposure time: 81s

  • Carrier free

    Anti-Metabotropic Glutamate Receptor 7/MGLUR7 antibody [EPR24530-11] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24530-11

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse, Rat

Applications

WB, IHC-Fr, IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody is unsuitable for human IHC-P and mouse IHC-FR.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ionotropic Glutamate receptor 2 + 3 antibody [EPR18115] (AB206293)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/50", "IHCFr-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>" } } }
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Product details

That antibody is unsuitable for human IHC-P and mouse IHC-FR. Also, ab302530 does not react in ICC and intracellular flow cytometry respectively with human and mouse+rat species.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

G-protein coupled receptor activated by glutamate that regulates axon outgrowth through the MAPK-cAMP-PKA signaling pathway during neuronal development (PubMed : 33500274). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase that it inhibits (PubMed : 9473604).
See full target information GRM7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

EMBO molecular medicine 16:506-522 PubMed38374465

2024

mGluR7 allosteric modulator AMN082 corrects protein synthesis and pathological phenotypes in FXS.

Applications

Unspecified application

Species

Unspecified reactive species

Vipendra Kumar,Kwan Young Lee,Anirudh Acharya,Matthew S Babik,Catherine A Christian-Hinman,Justin S Rhodes,Nien-Pei Tsai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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