AB192385

Anti-Metallothionein antibody

Name: Anti-Metallothionein antibody. Rabbit polyclonal (ab192385) | Abcam
Brand: Abcam Limited
SKU: AB192385
Rating: 3 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal MT2 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse samples. Cited in 13 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MT2A.

View Alternative Names

CES1, MT2, MT2A, Metallothionein-2, MT-2, Metallothionein-2A, Metallothionein-II, MT-II

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metallothionein antibody (AB192385)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metallothionein antibody (AB192385)

ab192385 staining Metallothionein in Mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with ab64226 for 10 minutes at room temperature; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/150) for 30 minutes. An undiluted HRP-conjugated Goat polyclonal (1/250) was used as the secondary antibody

Supplier Data

Western blot - Anti-Metallothionein antibody (AB192385)

Western blot analysis of extracts of HeLa cells useing ab192385.

All lanes:

Western blot - Anti-Metallothionein antibody (ab192385)

Predicted band size: 6 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human MT2A.

P02795

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p>" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab192385 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

See full target information MT2A

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

Journal of cachexia, sarcopenia and muscle 16:e70002 PubMed40745399

2025

Exogenous Nucleotides Supplementation Attenuates Age-Related Sarcopenia.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Wu,Rui Liu,Na Zhu,Xiujuan Wang,Chan Wei,Xiaoyang An,Meihong Xu,Yong Li

Cell reports. Medicine 6:101995 PubMed40107246

2025

BET inhibitor in combination with BCG vaccine enhances antitumor efficacy and orchestrates T cell reprogramming for melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wenhua Wang,Xin Li,Rui Hu,Liang Dong,Shiyao Pei,Liping Jin,Qian Gao,Xiang Chen,Mingzhu Yin

Journal of translational medicine 23:162 PubMed39915841

2025

MT2A promotes angiogenesis in chronically ischemic brains through a copper-mitochondria regulatory mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Mo,Chuyang Tai,Yang Yang,Cong Ling,Baoyu Zhang,Lei Wei,Cian Yao,Hui Wang,Chuan Chen

Veterinary pathology 61:815-828 PubMed38549443

2024

Pathology of "double scale" skin defect in farmed American alligators () and the possible association with hepatic fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Ilaria M Piras,Annemarie Bezuidenhout,Josué Díaz-Delgado,Deirdre Slawski,Pamela A Kelly

Neuroscience letters 818:137563 PubMed38036085

2023

Lumbosacral spinal proteomic changes during PAR4-induced persistent bladder pain.

Applications

Unspecified application

Species

Unspecified reactive species

Shaojing Ye,Nilesh M Agalave,Fei Ma,Dlovan F D Mahmood,Asma Al-Grety,Payam Emani Khoonsari,Camila I Svensson,Kim Kultima,Pedro L Vera

BMC zoology 8:21 PubMed37718412

2023

Evaluation of freshwater heavy metals accumulation effect on oxidative stress, Metallothionein biosynthesis and histopathology of Procambarus clarkii (Girard,1985) collected from three locations in the Delta region, Egypt.

Applications

Unspecified application

Species

Unspecified reactive species

Mahy M Mona,Mai L Younis,Aalaa I Atlam

Cancer cell international 22:205 PubMed35642057

2022

Metallothionein 2A (MT2A) controls cell proliferation and liver metastasis by controlling the MST1/LATS2/YAP1 signaling pathway in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Liu,Jun Quan,Zhaolong Shen,Zequn Zhang,Zhijian Chen,Liang Li,Xiaorong Li,Gui Hu,Xiaofeng Deng

International journal of molecular sciences 23: PubMed35563532

2022

Reducing Endogenous Labile Zn May Help to Reduce Smooth Muscle Cell Injury around Vascular Stents.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zeng,Yinhong Xie,Li Li,Huanran Wang,Jianying Tan,Xia Li,Qihao Bian,Yu Zhang,Tao Liu,Yajun Weng,Junying Chen

Saudi journal of biological sciences 28:7068-7076 PubMed34867008

2021

Edible bird's nest protects histomorphology of rat's uterus against cadmium (Cd) toxicity through a reduction of Cd deposition and enhanced antioxidant activity.

Applications

Unspecified application

Species

Unspecified reactive species

Abdul Quddus,Nurhusien Yimer,Faez Firdaus Abdullah Jesse,Muhammad Abdul Basit,Maria Amir,Mohammed Sirajul Islam

Redox biology 37:101746 PubMed33059313

2020

Copper interferes with selenoprotein synthesis and activity.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Schwarz,Kristina Lossow,Katja Schirl,Julian Hackler,Kostja Renko,Johannes Florian Kopp,Tanja Schwerdtle,Lutz Schomburg,Anna Patricia Kipp

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Metallothionein antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Metallothionein antibody (AB192385)

Western blot - Anti-Metallothionein antibody (AB192385)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (13)

Product promise