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Mouse Monoclonal MT1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rabbit samples. Cited in 57 publications. Immunogen corresponding to Full Length Protein corresponding to Rabbit MT1A_RABIT.


Images

Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (AB12228), expandable thumbnail
  • Western blot - Anti-Metallothionein antibody [UC1MT] (AB12228), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (AB12228), expandable thumbnail
  • Western blot - Anti-Metallothionein antibody [UC1MT] (AB12228), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (AB12228), expandable thumbnail

Publications

Key facts

Isotype

IgG1

Host species

Mouse

Storage buffer

Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 2.68% PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Full Length Protein corresponding to Rabbit MT1A_RABIT. The exact immunogen used to generate this antibody is proprietary information. Database link P11957

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Rabbit
Expected
Tested
Expected

Tested
Tested

Species

Human

Dilution info

-

Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Rabbit

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis.

Species

Rabbit

Dilution info

-

Notes

Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis.

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rabbit

Dilution info

Use at an assay dependent concentration.

Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.

Alternative names

Recommended products

Mouse Monoclonal MT1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rabbit samples. Cited in 57 publications. Immunogen corresponding to Full Length Protein corresponding to Rabbit MT1A_RABIT.

Alternative names

Key facts

Isotype

IgG1

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Full Length Protein corresponding to Rabbit MT1A_RABIT. The exact immunogen used to generate this antibody is proprietary information. Database link P11957
Clone number

UC1MT

Purity

Tissue culture supernatant

Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

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Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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5 product images

  • Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228), expandable thumbnail

    Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228)

    Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228), expandable thumbnail

    Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

    All lanes: Hela cell lysate

    Secondary

    All lanes: HRP-conjugated antibody.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 6 kDa

    Exposure time: 2min

  • Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

    ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228), expandable thumbnail

    Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

    This image was generated using the ascites version of the product.

    All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution

    All lanes: Rabbit liver lysates

    Predicted band size: 6 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

    ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

    This image was generated using the ascites version of the product.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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