Mouse Monoclonal MT1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rabbit samples. Cited in 57 publications. Immunogen corresponding to Full Length Protein corresponding to Rabbit MT1A_RABIT.
IgG1
Mouse
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 2.68% PBS
Liquid
Monoclonal
Flow Cyt | WB | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Rabbit | Expected | Tested | Expected |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
Species Rabbit | Dilution info - | Notes Please note: often Western blots done on cell lysates with this antibody produce many bands; we suspect that metallothionein binds to many other proteins, thus producing these results. As the predicted MW is around 6 kDa, use 12.5-20% gel and be sure the protein is not run off the gel during electrophoresis. |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Rabbit | Dilution info Use at an assay dependent concentration. | Notes - |
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Metallothioneins have a high content of cysteine residues that bind various heavy metals; these proteins are transcriptionally regulated by both heavy metals and glucocorticoids.
Metallothionein-1A, MT-1A, Metallothionein-IA, MT-IA, MT1A, MT1S
Mouse Monoclonal MT1A antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, Rabbit samples. Cited in 57 publications. Immunogen corresponding to Full Length Protein corresponding to Rabbit MT1A_RABIT.
Metallothionein-1A, MT-1A, Metallothionein-IA, MT-IA, MT1A, MT1S
IgG1
Mouse
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 2.68% PBS
Liquid
Monoclonal
UC1MT
Tissue culture supernatant
Purified from TCS.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
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Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)
All lanes: Hela cell lysate
All lanes: HRP-conjugated antibody.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 6 kDa
Exposure time: 2min
ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
This image was generated using the ascites version of the product.
All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution
All lanes: Rabbit liver lysates
Predicted band size: 6 kDa
ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-mouse IgG - H&L, pre-adsorbed (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
This image was generated using the ascites version of the product.
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