Anti-Metallothionein antibody [UC1MT]

5 product images

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  Flow Cytometry - Anti-Metallothionein antibody [UC1MT] (ab12228)

  Overlay histogram showing HeLA cells stained with ab12228 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab12228, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
  

  This image was generated using the ascites version of the product.

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  Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

  All lanes: Hela cell lysate

  Secondary

  All lanes: HRP-conjugated antibody.

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 6 kDa

  Exposure time: 2min

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  Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

  ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 1μg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor^® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  This image was generated using the ascites version of the product.

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  Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228)

  This image was generated using the ascites version of the product.

  All lanes: Western blot - Anti-Metallothionein antibody [UC1MT] (ab12228) at 1/1000 dilution

  All lanes: Rabbit liver lysates

  Predicted band size: 6 kDa

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Metallothionein antibody [UC1MT] (ab12228)

  ICC/IF image of ab12228 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12228, 10μg/ml) overnight at +4°C. The secondary antibody (green) was DyLight^® 488 goat anti-mouse IgG - H&L, pre-adsorbed (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) used at a 1/250 dilution for 1h. Alexa Fluor^® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
  

  This image was generated using the ascites version of the product.