AB123523

Anti-Methionine Aminopeptidase 2/p67 antibody

Name: Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523) | Abcam
Brand: Abcam Limited
SKU: AB123523
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal Methionine Aminopeptidase 2/p67 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human METAP2 aa 100-150.

View Alternative Names

MNPEP, P67EIF2, METAP2, Methionine aminopeptidase 2, MAP 2, MetAP 2, Initiation factor 2-associated 67 kDa glycoprotein, Peptidase M, p67, p67eIF2

2 Images

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Immunoprecipitation - Anti-Methionine Aminopeptidase 2/p67 antibody (AB123523)

Detection of Methionine Aminopeptidase 2/p67 by Western Blot of Immunprecipitate.
Lane 1 : ab123523 at 1μg/ml staining Methionine Aminopeptidase 2/p67 in HeLa whole cell lysate immunoprecipitated using ab123523 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2 : Control IgG
Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523)

Predicted band size: 52 kDa

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Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody (AB123523)

All lanes:

Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody (ab123523) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 52 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human METAP2 aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

P50579

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Ferret": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The protein expressed by the MAP2 gene cotranslationally removes the N-terminal methionine from nascent proteins, particularly when the second residue is small and uncharged. In comparison to METAP1, the catalytic activity of human METAP2 towards Met-Val peptides is consistently much higher, indicating its role in processing proteins with N-terminal Met-Val and Met-Thr sequences in vivo. Additionally, it protects the eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases, such as EIF2AK2/PKR and EIF2AK1/HCR, playing a critical role in the regulation of protein synthesis. This supplementary information is collated from multiple sources and compiled automatically.

See full target information METAP2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Neural regeneration research 19:900-907 PubMed37843227

2023

Human umbilical cord mesenchymal stem cell-derived exosomes loaded into a composite conduit promote functional recovery after peripheral nerve injury in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Haoshuai Tang,Junjin Li,Hongda Wang,Jie Ren,Han Ding,Jun Shang,Min Wang,Zhijian Wei,Shiqing Feng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Methionine Aminopeptidase 2/p67 antibody

Immunoprecipitation - Anti-Methionine Aminopeptidase 2/p67 antibody (AB123523)

Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody (AB123523)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (1)

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