Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (AB134124)

Immunohistochemical analysis of paraffin embedded Human colon tissue labelling Methionine Aminopeptidase 2/p67 with ab134124 at 1/250.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (AB134124)

Overlay histogram showing HeLa cells stained with ab134124 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab134124, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor^® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

• WB

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Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (AB134124)

All lanes:

Western blot - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (ab134124) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

Caco 2 cell lysate at 10 µg

Lane 3:

HepG2 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

Predicted band size: 52 kDa

Observed band size: 67 kDa

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• OI-RD Scanning

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OI-RD Scanning - Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6887] (AB134124)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.