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AB250831

Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free

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Rabbit Recombinant Monoclonal Methionyl Aminopeptidase 1/MAP 1 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P and reacts with Human, Rat samples.

View Alternative Names

KIAA0094, METAP1, Methionine aminopeptidase 1, MAP 1, MetAP 1, Peptidase M 1

6 Images
Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®488) at a 1/200 dilution. Counterstained with Dapi blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Flow Cytometry (Intracellular) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.

Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor®555) at a 1/200 dilution. Counterstained with Dapi blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1/MAP 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)
  • WB

Supplier Data

Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] - BSA and Azide free (AB250831)

This data was developed using ab185540, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)] (<a href='/en-us/products/primary-antibodies/methionyl-aminopeptidase-1-map-1-antibody-epr15452b-ab185540'>ab185540</a>) at 1/1000 dilution

Lane 1:

A375 cell lysate at 10 µg

Lane 2:

HUVEC cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

  • Unconjugated

    Anti-Methionyl Aminopeptidase 1/MAP 1 antibody [EPR15452(B)]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15452(B)

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Reactivity data

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Product details

ab250831 is the carrier-free version of ab185540.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.
See full target information METAP1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com