AB23366

Anti-Methylated Lysine (di methyl , mono methyl ) antibody

Name: Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366) | Abcam
Brand: Abcam Limited
SKU: AB23366
Price: 645 USD
Availability: InStock
Rating: 4 (8 reviews)

(8 Reviews)

(55 Publications)

Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366) is a rabbit polyclonal antibody detecting Methylated Lysine in Western Blot, IHC-P, ELISA.

- Over 40 publications
- Trusted since 2005

View Alternative Names

Dimethylysine, Methyl lysine, N epsilon dimethyl lysine, N epsilon mono methyllysine, N epsilon trimethyl lysine, N(epsilon) trimethyl lysine, Trimethyl lysine

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

ab23366 staining formalin fixed paraffin-embedded rat kidney tissue sections. The section was subjected to heat mediated antigen retrieval in citrate buffer (pH 6.0) and blocked with 5% serum for 30 minutes at 20°C, prior to incubation with the primary antibody (diluted 1/200 in PBS + 5% serum) for 45 mintes at 20°C. An HRP-conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

IHC-P

AbReview9022****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

ab23366 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed a heat mediated antigen retrieval step was performed in citrate buffer. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

This image is courtesy of an anonymous Abreview

Supplier Data

Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

Lane 1:

Anti-Methylated Lysine (tri methyl) antibody (does not cross react with mono, or di methyl lysine residues)

Lane 2:

Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366)

All lanes:

Yeast extract

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Applications

ELISA, IHC-P, WB

applications

Specificity

Recognize proteins methylated on lysine residues (mono- & di-methyl lysine). This antibody was affinity purified with mono-methyl lysine and di-methyl lysine, and may also react with tri-methyl lysine. Does not cross-react with acetylated protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Modified Amino Acid": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "Do not use milk for any of the buffers as complex milk proteins may contain competitive methylated proteins. Use BSA instead. ab23366 incubation time 2h RT. If no or weak signal is obtained overnight at 4deg might help.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23366) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), ELISA. Trusted by the scientific community
Anti-Methylated Lysine (di methyl , mono methyl ) (ab23366) was first used in a scientific publication in 2005 and has been cited over 40 times in peer-reviewed journals.

Reviewed by scientists
Anti-Methylated Lysine (di methyl , mono methyl ) (ab23366) has over 5 independent reviews from customers.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Affinity purified using N-methyl (epsilon amino group)lysine on Agarose as affinity matrix.

Storage buffer

pH: 6 - 8 Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Methylated Lysine

Additional targets

Methylated Lysine,Methylated Lysine,Methylated Lysine

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Publications (55)

Recent publications for all applications. Explore the full list and refine your search

Stem cell research & therapy 16:498 PubMed40999515

2025

SUV39H1 facilitate the osteogenic differentiation of dental pulp stem cells by modulating lipid metabolism and mitochondrial dynamics through FASN via non-histone methylation and ubiquitination mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Wang,Wanqing Wang,Xiao Han,Yangyang Cao,Haoqing Yang,Chen Zhang,Huina Liu,Mengyuan Zhu,Dengsheng Xia,Zhipeng Fan

Scientific reports 15:31643 PubMed40866490

2025

RAD18 methylation by the methyltransferase SETD6 attenuates DNA breaks.

Applications

Unspecified application

Species

Unspecified reactive species

Lital Estrella Weil,Michal Feldman,Jennifer Van Duine,Ji Qiu,Joshua LaBaer,Dan Levy

Journal of translational medicine 22:979 PubMed39472935

2024

EZH2 inhibition or genetic ablation suppresses cyst growth in autosomal dominant polycystic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Lv,Bingxue Lan,Lili Fu,Chaoran He,Wei Zhou,Xi Wang,Chenchen Zhou,Zhiguo Mao,Yupeng Chen,Changlin Mei,Cheng Xue

The Journal of cell biology 223: PubMed39007857

2024

Dual protection by Bcp1 and Rkm1 ensures incorporation of uL14 into pre-60S ribosomal subunits.

Applications

Unspecified application

Species

Unspecified reactive species

Min-Chi Yeh,Ning-Hsiang Hsu,Hao-Yu Chu,Cheng-Han Yang,Pang-Hung Hsu,Chi-Chi Chou,Jing-Ting Shie,Wei-Ming Lee,Meng-Chiao Ho,Kai-Yin Lo

Haematologica 109:725-739 PubMed37317878

2024

Aberrant MNX1 expression associated with t(7;12)(q36;p13) pediatric acute myeloid leukemia induces the disease through altering histone methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed Waraky,Anders Östlund,Tina Nilsson,Dieter Weichenhan,Pavlo Lutsik,Marion Bähr,Joschka Hey,Gürcan Tunali,Jenni Adamsson,Susanna Jacobsson,Mohammad Hamdy Abdelrazak Morsy,Susann Li,Linda Fogelstrand,Christoph Plass,Lars Palmqvist

Oncogenesis 13:3 PubMed38191478

2024

SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Huang,Ming Tang,Zhiyi Hu,Bailian Cai,Guofang Chen,Lijun Jiang,Yan Xia,Pujun Guan,Xiaoqi Li,Zhiyong Mao,Xiaoping Wan,Wen Lu

EMBO reports 25:198-227 PubMed38177908

2024

TTLL12 is required for primary ciliary axoneme formation in polarized epithelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Julia Ceglowski,Huxley K Hoffman,Andrew J Neumann,Katie J Hoff,Bailey L McCurdy,Jeffrey K Moore,Rytis Prekeris

Science advances 9:eade6624 PubMed37315132

2023

SMYD2 inhibition-mediated hypomethylation of Ku70 contributes to impaired nonhomologous end joining repair and antitumor immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Tang,Guofang Chen,Bo Tu,Zhiyi Hu,Yujia Huang,Christopher C DuFort,Xiaoping Wan,Zhiyong Mao,Yongzhong Liu,Wei-Guo Zhu,Wen Lu

Proceedings of the National Academy of Sciences of the United States of America 120:e2208941120 PubMed36656859

2023

Structural remodeling of AAA+ ATPase p97 by adaptor protein ASPL facilitates posttranslational methylation by METTL21D.

Applications

Unspecified application

Species

Unspecified reactive species

Saša Petrović,Yvette Roske,Biria Rami,Mai Hoang Quynh Phan,Daniela Panáková,Udo Heinemann

Biomolecules 12: PubMed35740908

2022

Unique SMYD5 Structure Revealed by AlphaFold Correlates with Its Functional Divergence.

Applications

Unspecified application

Species

Unspecified reactive species

Yingxue Zhang,Eid Alshammari,Jacob Sobota,Alexander Yang,Chunying Li,Zhe Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Methylated Lysine (di methyl , mono methyl ) antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

Western blot - Anti-Methylated Lysine (di methyl , mono methyl ) antibody (AB23366)

Key facts

Host species

Clonality

Isotype

Carrier free

Applications

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Additional targets

Publications (55)

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