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AB243074

Anti-Methylglyoxal antibody [9F11]

5

(2 Reviews)

|

(10 Publications)

Mouse Monoclonal Methylglyoxal antibody. Suitable for WB, Flow Cyt and reacts with Chemical samples. Cited in 10 publications. Immunogen corresponding to Chemical / Small Molecule corresponding to Methylglyoxal.

View Alternative Names

2 oxo propanal, pyruvaldehyde

2 Images
Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (AB243074)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Methylglyoxal antibody [9F11] (AB243074)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+ 250 μM hydrogen peroxide treatment for 24 hours) labeling Methylglyoxal with ab243074 at 1/50 dilution for 30 minutes on ice (solid line) compared with an Isotype control IgG (dashed line). A Goat Anti-Mouse : PE at 1/100 dilution was used as the secondary antibody (20 mins at RT).

Western blot - Anti-Methylglyoxal antibody [9F11] (AB243074)
  • WB

Ap

Western blot - Anti-Methylglyoxal antibody [9F11] (AB243074)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Methylglyoxal antibody [9F11] (ab243074) at 1/1000 dilution

Lane 1:

Methylglyoxal-BSA at 0.5 µg

Lane 2:

BSA at 0.5 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

9F11

Isotype

IgG1

Carrier free

No

Applications

WB, Flow Cyt

applications

Specificity

Specific for Methylglyoxal modified proteins. Does not detect free Methylglyoxal. Does not cross-react with Acrolein, Hexanoyl Lysine, MDA, 4-HHE, 4-HNE, or Crotonaldehyde modified proteins.

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Primary Antibodies

AB125145

Anti-Carboxymethyl Lysine antibody [CML26]

Flow Cytometry - Anti-Carboxymethyl Lysine antibody [CML26] (AB125145)

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Primary Antibodies

AB27684

Anti-Carboxymethyl Lysine antibody

Western blot - Anti-Carboxymethyl Lysine antibody (AB27684)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Chemical": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/50", "FlowCyt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from TCS.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Methylglyoxal
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed39769037

2025

L. Leaf Extract Dose-Dependently Modulates Oxidative Stress in the Kidney and Exerts Anti-Fibrotic and Anti-Inflammatory Properties by the Molecular Mechanisms Independent of NRF-2 Signalization Mirroring the Effects of Losartan in SHR.

Applications

Unspecified application

Species

Unspecified reactive species

Una-Jovana Vajic,Nevena Mihailovic-Stanojevic,Danijela Karanovic,Maja Zivotic,Milan Ivanov,Djurdjica Jovovic,Jelica Grujic-Milanovic,Zoran Miloradovic

Molecules (Basel, Switzerland) 29: PubMed39519689

2024

Methylglyoxal-Induced Modifications in Human Triosephosphate Isomerase: Structural and Functional Repercussions of Specific Mutations.

Applications

Unspecified application

Species

Unspecified reactive species

Ignacio de la Mora-de la Mora,Itzhel García-Torres,Luis Antonio Flores-López,Gabriel López-Velázquez,Gloria Hernández-Alcántara,Saúl Gómez-Manzo,Sergio Enríquez-Flores

Redox biology 65:102843 PubMed37573838

2023

Methylglyoxal suppresses microglia inflammatory response through NRF2-IκBζ pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Li Wei,Ying Yang,Wei-Yue Si,Yang Zhou,Tao Li,Tong Du,Peng Zhang,Xiao-Li Li,Ruo-Nan Duan,Rui-Sheng Duan,Chun-Lin Yang

eLife 12: PubMed37548361

2023

DJ-1 paralogs maintain genome integrity through glycation repair of nucleic acids and proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Gautam Susarla,Priyanka Kataria,Amrita Kundu,Patrick D'Silva

International journal of molecular sciences 24: PubMed37298498

2023

Metformin Counteracts the Deleterious Effects of Methylglyoxal on Ovalbumin-Induced Airway Eosinophilic Inflammation and Remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Matheus L Medeiros,Akila L Oliveira,Glaucia C Mello,Edson Antunes

Amino acids 55:413-420 PubMed36637533

2023

Carnosine increases insulin-stimulated glucose uptake and reduces methylglyoxal-modified proteins in type-2 diabetic human skeletal muscle cells.

Applications

Unspecified application

Species

Unspecified reactive species

Joseph J Matthews,Mark D Turner,Livia Santos,Kirsty J Elliott-Sale,Craig Sale

Journal of molecular and cellular cardiology 174:101-114 PubMed36481511

2022

Distinct effects of intracellular vs. extracellular acidic pH on the cardiac metabolome during ischemia and reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander S Milliken,Jessica H Ciesla,Sergiy M Nadtochiy,Paul S Brookes

The New phytologist 236:1061-1074 PubMed35976797

2022

Double DJ-1 domain containing Arabidopsis DJ-1D is a robust macromolecule deglycase.

Applications

Unspecified application

Species

Unspecified reactive species

Melvin Prasad,Priyanka Kataria,Sunayana Ningaraju,Radhika Buddidathi,Kondalarao Bankapalli,Chenna Swetha,Gautam Susarla,Radhika Venkatesan,Patrick D'Silva,Padubidri V Shivaprasad

eLife 9: PubMed32795389

2020

ALKBH7 mediates necrosis via rewiring of glyoxal metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Chaitanya A Kulkarni,Sergiy M Nadtochiy,Leslie Kennedy,Jimmy Zhang,Sophea Chhim,Hanan Alwaseem,Elizabeth Murphy,Dragony Fu,Paul S Brookes

Cancers 12: PubMed32466621

2020

Genetic Target Modulation Employing CRISPR/Cas9 Identifies Glyoxalase 1 as a Novel Molecular Determinant of Invasion and Metastasis in A375 Human Malignant Melanoma Cells In Vitro and In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Jandova,Jessica Perer,Anh Hua,Jeremy A Snell,Georg T Wondrak
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

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