Mouse Polyclonal Methylmalonyl Coenzyme A mutase antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MMUT.
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Publications
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- Journal of inherited metabolic disease 46:421-4352022Cellular and computational models reveal environmental and metabolic interactions in MMUT-type methylmalonic aciduria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharlotte Ramon,Florian Traversi,Céline Bürer,D Sean Froese,Jörg StellingPubMed 36371683
- Nature communications 11:9702020Impaired mitophagy links mitochondrial disease to epithelial stress in methylmalonyl-CoA mutase deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlessandro Luciani et. al.PubMed 32080200
- The Journal of biological chemistry 291:20563-732016Novel Mouse Models of Methylmalonic Aciduria Recapitulate Phenotypic Traits with a Genetic Dosage Effect.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatrick Forny et. al.PubMed 27519416
- Human molecular genetics 24:7049-592015Molecular and biochemical alterations in tubular epithelial cells of patients with isolated methylmalonic aciduria.Applications:Unspecified applicationReactive species:Unspecified reactive speciesT Ruppert et. al.PubMed 26420839
- Human gene therapy 25:529-382014Correction of methylmalonic aciduria in vivo using a codon-optimized lentiviral vector.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEdward S Y Wong et. al.PubMed 24568291
- Neuroscience 164:578-872009Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human MMUT. Database link P22033
Consider this alternative
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Expected | Tested |
| Rat | Predicted | Expected | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.
Alternative names
MUT, MMUT, MCM, Methylmalonyl-CoA isomerase
Recommended products
Mouse Polyclonal Methylmalonyl Coenzyme A mutase antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Rat samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human MMUT.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human MMUT. Database link P22033
- Carrier free
- Yes
- Purification technique
- Affinity purification Protein G
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)
All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution
All lanes: human liver tissue lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)
All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/2000 dilution
Lane 1: Euglena whole cell lysate at 15 µg with Milk and 0.05% Tween20
Lane 2: Euglena (RNAi gene silencing Methylmalonyl Coenzyme A mutase) whole cell lysate, at 15 µg with Milk and 0.05% Tween20
SecondaryAll lanes: Goat Anti-mouse IgG(H+L) HRP conjugated at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 83 kDa
Exposure time: 10min
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)
All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution
Lane 1: Methylmalonyl Coenzyme A mutase transfected 293T cell lysate at 25 µg
Lane 2: Non-transfected 293T cell lysate at 25 µg
SecondaryAll lanes: Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)
ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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