Anti-Methylmalonyl Coenzyme A mutase antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody (AB67869)

ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

AbReview62163****

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (AB67869)

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/2000 dilution

Lane 1:

Euglena whole cell lysate at 15 µg

Lane 2:

Euglena (RNAi gene silencing Methylmalonyl Coenzyme A mutase) whole cell lysate, at 15 µg

Secondary

All lanes:

Goat Anti-mouse IgG(H+L) HRP conjugated at 1/5000 dilution

Predicted band size: 83 kDa

false

Exposure time: 10min

• WB

Unknown

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (AB67869)

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution

Lane 1:

Methylmalonyl Coenzyme A mutase transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

• WB

Unknown

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (AB67869)

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution

All lanes:

human liver tissue lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false