Rabbit Recombinant Monoclonal Methylmalonyl Coenzyme A mutase antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 7 publications.
Images
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img7260.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105607.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)")
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img11897.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133716.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)")
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img7227.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (AB134956)")
Publications
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- PloS one 17:e02747742022Novel AAV-mediated genome editing therapy improves health and survival in a mouse model of methylmalonic acidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShengwen Zhang,Amy Bastille,Susana Gordo,Nikhil Ramesh,Jenisha Vora,Elizabeth McCarthy,Xiaohan Zhang,Dylan Frank,Chih-Wei Ko,Carmen Wu,Noel Walsh,Shreya Amarwani,Jing Liao,Qiang Xiong,Lauren Drouin,Matthias Hebben,Kyle Chiang,B Nelson ChauPubMed 36126056
- Molecular therapy. Methods & clinical development 27:61-722022Systemic gene therapy for methylmalonic acidemia using the novel adeno-associated viral vector 44.9.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRandy J Chandler,Giovanni Di Pasquale,Jennifer L Sloan,Samantha McCoy,Brandon T Hubbard,Tina M Kilts,Irini Manoli,John A Chiorini,Charles P VendittiPubMed 36186952
- Nature metabolism 4:435-4432022Altered propionate metabolism contributes to tumour progression and aggressiveness.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAna P Gomes,Didem Ilter,Vivien Low,Stanislav Drapela,Tanya Schild,Edouard Mullarky,Julie Han,Ilaria Elia,Dorien Broekaert,Adam Rosenzweig,Michal Nagiec,Joana B Nunes,Bethany E Schaffer,Anders P Mutvei,John M Asara,Lewis C Cantley,Sarah-Maria Fendt,John BlenisPubMed 35361954
- Molecular therapy. Methods & clinical development 23:619-6322021genome editing at the albumin locus to treat methylmalonic acidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJessica L Schneller,Ciaran M Lee,Leah E Venturoni,Randy J Chandler,Ang Li,Sangho Myung,Thomas J Cradick,Ayrea E Hurley,William R Lagor,Gang Bao,Charles P VendittiPubMed 34901307
- Molecular therapy. Methods & clinical development 21:765-7762021Central nervous system-targeted adeno-associated virus gene therapy in methylmalonic acidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancis J May,PamelaSara E Head,Leah E Venturoni,Randy J Chandler,Charles P VendittiPubMed 34169115
- Hepatology (Baltimore, Md.) 73:2223-22372020Promoterless, Nuclease-Free Genome Editing Confers a Growth Advantage for Corrected Hepatocytes in Mice With Methylmalonic Acidemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRandy J Chandler,Leah E Venturoni,Jing Liao,Brandon T Hubbard,Jessica L Schneller,Victoria Hoffmann,Susana Gordo,Shengwen Zang,Chih-Wei Ko,Nelson Chau,Kyle Chiang,Mark A Kay,Adi Barzel,Charles P VendittiPubMed 32976669
- Cell metabolism 30:352-363.e82019Polyamines and eIF5A Hypusination Modulate Mitochondrial Respiration and Macrophage Activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDaniel J Puleston et. al.PubMed 31130465
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Not recommended | Tested | Not recommended |
| Cow | Predicted | Not recommended | Not recommended | Predicted | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/50 - 1/100 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species Mouse | Dilution info 1/50 - 1/100 | Notes The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
Species Rat | Dilution info 1/1000 - 1/10000 | Notes - |
Species Mouse | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human, Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Catalyzes the reversible isomerization of methylmalonyl-CoA (MMCoA) (generated from branched-chain amino acid metabolism and degradation of dietary odd chain fatty acids and cholesterol) to succinyl-CoA (3-carboxypropionyl-CoA), a key intermediate of the tricarboxylic acid cycle.
Alternative names
MUT, MMUT, MCM, Methylmalonyl-CoA isomerase
Recommended products
Rabbit Recombinant Monoclonal Methylmalonyl Coenzyme A mutase antibody. Suitable for IHC-P, WB and reacts with Human, Rat, Mouse samples. Cited in 7 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR7738
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img7260.jpg "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
Lane 1: Wild-type HAP1 cell lysate (20 μg)
Lane 2: Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 μg)
Lane 3: K562 cell lysate (20 μg)
Lane 4: Human liver tissue lysate (20 μg)
Lanes 1 - 4: Merged signal (red and green). Green - ab134956 (unpurified) observed at 85 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab134956 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab134956 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
Predicted band size: 83 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img105607.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Methylmalonyl Coenzyme A mutase with purified ab134956 at 1:50 dilution (2.96 μg/ml). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img11897.jpg "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/2000 dilution
Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg
Lane 3: Mouse brain lysates at 20 µg
Lane 4: Rat brain lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 83 kDa
Observed band size: 83 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img133716.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at 1/50 dilution.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--western-blot-img7227.jpg "Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
All lanes: Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: K562 cell lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: Human fetal liver tissue lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti rabbit at 1/2000 dilution
Predicted band size: 83 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127863.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 (unpurified) at dilution 1/50.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956), expandable thumbnail](https://content.abcam.com/products/images/methylmalonyl-coenzyme-a-mutase-antibody-epr7738-ab134956--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img127863.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] (ab134956)")
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