Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 at dilution 1/50.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134956) (unpurified).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling Methylmalonyl Coenzyme A mutase with ab134956 at 1/50 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134956) (unpurified).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human kidney tissue sections labeling Methylmalonyl Coenzyme A mutase with purified ab134956 at 1/50 dilution (2.96 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134956).

• WB

Unknown

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

This data was developed using ab134956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (ab240091) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg

Lane 2:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg

Lanes 3 - 4:

Mouse brain lysates at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

• WB

Unknown

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

This data was developed using ab134956, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (ab240091) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

K562 cell lysate at 10 µg

Lane 3:

293T cell lysate at 10 µg

Lane 4:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti rabbit at 1/2000 dilution

Predicted band size: 83 kDa

false

• WB

Lab

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (AB240091)

This data was developed using ab134956, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 μg)
Lane 3 : K562 cell lysate (20 μg)
Lane 4 : Human liver tissue lysate (20 μg)
Lanes 1 - 4 : Merged signal (red and green). Green - ab134956 (unpurified) observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab134956 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab134956 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7738] - BSA and Azide free (ab240091)

Predicted band size: 83 kDa

false