Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (AB236068)

Immunofluorescent staining of HeLa cells labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/100.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133672).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (AB236068)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1 : 100 dilution (8.7 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0)Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)was used as the secondary antibody.Negative control : PBS instead of the primary antibody.Hematoxylinwas used as a counterstainThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133672).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (AB236068)

Immunohistochemical analysis of paraffin embedded Human kidney tissue labelling Methylmalonyl Coenzyme A mutase with unpurified ab133672 at 1/50.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133672).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (AB236068)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Methylmalonyl Coenzyme A mutase with Purified ab133672 at 1 : 100 dilution (9.9 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133672).

• WB

Lab

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] - BSA and Azide free (AB236068)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : Methylmalonyl Coenzyme A mutase knockout HAP1 cell lysate (20 μg)
Lane 3 : HeLa cell lysate (20 μg)
Lane 4 : K562 cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab133672 observed at 85 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab133672 was shown to specifically react with Methylmalonyl Coenzyme A mutase when Methylmalonyl Coenzyme A mutase knockout samples were used. Wild-type and Methylmalonyl Coenzyme A mutase knockout samples were subjected to SDS-PAGE. ab133672 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab133672).

All lanes:

Western blot - Anti-Methylmalonyl Coenzyme A mutase antibody [EPR7739] (<a href='/en-us/products/primary-antibodies/methylmalonyl-coenzyme-a-mutase-antibody-epr7739-ab133672'>ab133672</a>)

Predicted band size: 83 kDa

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