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AB3823

Anti-Metnase antibody

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(5 Publications)

Goat Polyclonal Metnase antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human SETMAR aa 650 to C-terminus.

View Alternative Names

Histone-lysine N-methyltransferase SETMAR, SET domain and mariner transposase fusion protein, Metnase, SETMAR

1 Images
Western blot - Anti-Metnase antibody (AB3823)
  • WB

Unknown

Western blot - Anti-Metnase antibody (AB3823)

ab3823 staining (2µg/ml) of Daudi lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 12 hours. Detected by western blot using chemiluminescence.

All lanes:

Western blot - Anti-Metnase antibody (ab3823)

Predicted band size: 77 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Synthetic Peptide within Human SETMAR aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q53H47

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product. Approx 70 - 75 kDa band seen in Daudi lysate.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity (PubMed : 16332963, PubMed : 16672366, PubMed : 17403897, PubMed : 17877369, PubMed : 18263876, PubMed : 20521842, PubMed : 22231448, PubMed : 24573677). In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining (PubMed : 16332963, PubMed : 21187428, PubMed : 22231448). Also regulates replication fork processing, promoting replication fork restart and regulating DNA decatenation through stimulation of the topoisomerase activity of TOP2A (PubMed : 18790802, PubMed : 20457750).
See full target information SETMAR
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Genomics 113:1589-1604 PubMed33812898

2021

Two repeated motifs enriched within some enhancers and origins of replication are bound by SETMAR isoforms in human colon cells.

Applications

Unspecified application

Species

Unspecified reactive species

Aymeric Antoine-Lorquin,Peter Arensburger,Ahmed Arnaoty,Sassan Asgari,Martine Batailler,Linda Beauclair,Catherine Belleannée,Nicolas Buisine,Vincent Coustham,Serge Guyetant,Laura Helou,Thierry Lecomte,Bruno Pitard,Isabelle Stévant,Yves Bigot

NAR cancer 2:zcaa008 PubMed32743552

2020

Distinct roles of structure-specific endonucleases EEPD1 and Metnase in replication stress responses.

Applications

Unspecified application

Species

Unspecified reactive species

Neelam Sharma,Michael C Speed,Christopher P Allen,David G Maranon,Elizabeth Williamson,Sudha Singh,Robert Hromas,Jac A Nickoloff

Mobile DNA 11:5 PubMed31938044

2020

Compensating for over-production inhibition of the Hsmar1 transposon in using a series of constitutive promoters.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Tellier,Ronald Chalmers

DNA repair 80:26-35 PubMed31238295

2019

The roles of the human SETMAR (Metnase) protein in illegitimate DNA recombination and non-homologous end joining repair.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Tellier,Ronald Chalmers

Retrovirology 5:40 PubMed18498648

2008

Lysine methylation of HIV-1 Tat regulates transcriptional activity of the viral LTR.

Applications

WB

Species

Human

Rachel Van Duyne,Rebecca Easley,Weilin Wu,Reem Berro,Caitlin Pedati,Zachary Klase,Kylene Kehn-Hall,Elizabeth K Flynn,David E Symer,Fatah Kashanchi
View all publications
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Product promise

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