AB50793

Anti-MetRS/MARS antibody [MARSD10B4]

Name: Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) | Abcam
Brand: Abcam Limited
SKU: AB50793
Price: 500 USD
Availability: InStock

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(11 Publications)

Mouse Monoclonal MetRS/MARS antibody. Suitable for IP, Flow Cyt, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 11 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MARS1 aa 50-200.

View Alternative Names

MARS, MARS1, Methionyl-tRNA synthetase, MetRS

6 Images

Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Flow Cyt

Unknown

Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.

Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.

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Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.

All lanes:

Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 5 µg

All lanes:

RIPA lysate of HeLa cells at 200 µg

Predicted band size: 101 kDa

Observed band size: 110 kDa

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Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

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Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 101 kDa

Observed band size: 98 kDa

false

Unknown

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

All lanes:

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 101 kDa

Observed band size: 98 kDa

false

Unknown

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

All lanes:

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution

All lanes:

F2408 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody

Predicted band size: 101 kDa

Observed band size: 98 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MARSD10B4

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IP, WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human MARS1 aa 50-200. The exact immunogen used to generate this antibody is proprietary information.

P56192

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "5 µg/mg of lysate", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/10", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/10", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Ab50793 was filtered through an 0.22 µm membrane.

Storage buffer

pH: 7.4 Preservative: 0.05% Sodium azide Constituents: 1% BSA, 0.1312% Sodium phosphate, 0.03% Tripotassium orthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction : the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA (PubMed : 11714285). Plays a role in the synthesis of ribosomal RNA in the nucleolus (PubMed : 10791971).

See full target information MARS1

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

Molecular and cellular endocrinology 584:112160 PubMed38266771

2024

PMM2 controls ERα levels and cell proliferation in ESR1 Y537S variant expressing breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Manuela Cipolletti,Filippo Acconcia

Molecular and cellular biology 43:223-240 PubMed37154023

2023

EPRS1 Controls the TGF- Signaling Pathway via Interaction with TβRI in Hepatic Stellate Cell.

Applications

Unspecified application

Species

Unspecified reactive species

Ina Yoon,Ji Ae Song,Ji Hun Suh,Sulhee Kim,Jonghyeon Son,Jong Hyun Kim,Song Yee Jang,Kwang Yeon Hwang,Myung Hee Kim,Sunghoon Kim

Cell reports. Medicine 4:100953 PubMed36809766

2023

Gestational palmitic acid suppresses embryonic GATA-binding protein 4 signaling and causes congenital heart disease.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Li Cao,Wen-Jun Gu,Lei Li,Zhong-Zhong Chen,Jie Xiang,Ze-Yu Zhou,Bo Xu,Wei-Dong Zang,Xiang-Yu Zhou,Jing Cao,Kun Sun,Jian-Yuan Zhao

Nature communications 13:6455 PubMed36309524

2022

Glutamyl-prolyl-tRNA synthetase 1 coordinates early endosomal anti-inflammatory AKT signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Eun-Young Lee,Su-Man Kim,Jung Hwan Hwang,Song Yee Jang,Shinhye Park,Sanghyeon Choi,Ga Seul Lee,Jungwon Hwang,Jeong Hee Moon,Paul L Fox,Sunghoon Kim,Chul-Ho Lee,Myung Hee Kim

Proceedings of the National Academy of Sciences of the United States of America 118: PubMed33568529

2021

Local translation in nuclear condensate amyloid bodies.

Applications

Unspecified application

Species

Unspecified reactive species

Phaedra R Theodoridis,Michael Bokros,Dane Marijan,Nathan C Balukoff,Dazhi Wang,Chloe C Kirk,Taylor D Budine,Harris D Goldsmith,Miling Wang,Timothy E Audas,Stephen Lee

Cell reports 25:398-412.e6 PubMed30304680

2018

Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair.

Applications

Unspecified application

Species

Unspecified reactive species

Dan Wang,Rui Zhao,Yuan-Yuan Qu,Xin-Yu Mei,Xuan Zhang,Qian Zhou,Yang Li,Shao-Bo Yang,Zhi-Gui Zuo,Yi-Ming Chen,Yan Lin,Wei Xu,Chao Chen,Shi-Min Zhao,Jian-Yuan Zhao

ACS pharmacology & translational science 1:21-31 PubMed32219202

2018

Stabilization of Cyclin-Dependent Kinase 4 by Methionyl-tRNA Synthetase in p16-Negative Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Nam Hoon Kwon,Jin Young Lee,Ye-Lim Ryu,Chanhee Kim,Jiwon Kong,Seongeun Oh,Beom Sik Kang,Hye Won Ahn,Sung Gwe Ahn,Joon Jeong,Hoi Kyoung Kim,Jong Hyun Kim,Dae Young Han,Min Chul Park,Doyeun Kim,Ryuichi Takase,Isao Masuda,Ya-Ming Hou,Sung Ill Jang,Yoon Soo Chang,Dong Ki Lee,Youngeun Kim,Ming-Wei Wang, Basappa,Sunghoon Kim

MethodsX 5:75-82 PubMed30619721

2017

A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2α.

Applications

Unspecified application

Species

Unspecified reactive species

Richard C Silva,Beatriz A Castilho,Evelyn Sattlegger

PloS one 10:e0142253 PubMed26544075

2015

Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography.

Applications

Species

Human

Seong-Jun Park,Hee-Sung Ahn,Jun Seok Kim,Cheolju Lee

Proceedings of the National Academy of Sciences of 110:2557-62 PubMed23359686

2013

Role for the obesity-related FTO gene in the cellular sensing of amino acids.

Applications

IP, WB

Species

Mouse, Mouse

Pawan Gulati,Man Ka Cheung,Robin Antrobus,Chris D Church,Heather P Harding,Yi-Chun Loraine Tung,Debra Rimmington,Marcella Ma,David Ron,Paul J Lehner,Frances M Ashcroft,Roger D Cox,Anthony P Coll,Stephen O'Rahilly,Giles S H Yeo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MetRS/MARS antibody [MARSD10B4]

Flow Cytometry - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Immunocytochemistry/ Immunofluorescence - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Immunoprecipitation - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Western blot - Anti-MetRS/MARS antibody [MARSD10B4] (AB50793)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Product protocols

Target data

Publications (11)

Product promise