AB157097

Anti-METTL1 antibody

Name: Anti-METTL1 antibody (ab157097) | Abcam
Brand: Abcam Limited
SKU: AB157097
Price: 520 USD
Availability: InStock

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(4 Publications)

Rabbit Polyclonal METTL1 phospho S27 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human METTL1 phospho S27 aa 150-250.

View Alternative Names

C12orf1, METTL1, tRNA (guanine-N(7)-)-methyltransferase, Methyltransferase-like protein 1, mRNA (guanine-N(7)-)-methyltransferase, miRNA (guanine-N(7)-)-methyltransferase, tRNA (guanine(46)-N(7))-methyltransferase, tRNA(m7G46)-methyltransferase

1 Images

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Western blot - Anti-METTL1 antibody (AB157097)

All lanes:

Western blot - Anti-METTL1 antibody (ab157097) at 0.4 µg/mL

Lane 1:

293T whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Predicted band size: 31 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human METTL1 phospho S27 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

Q9UBP6

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL1 also known as Methyltransferase-Like 1 is an enzyme responsible for catalyzing the methylation of specific substrates. This protein has a molecular mass of approximately 50 kDa. METTL1 is expressed across various tissues but shows higher expression levels in the brain and testes. Its enzymatic activity involves transferring a methyl group through its interaction with S-adenosylmethionine (SAM) to form N7-methylguanosine (m7G) on tRNA molecules which is essential for regulating their stability and function.

Biological function summary

METTL1 is important in modulating the processes of cellular growth and differentiation. METTL1 forms a complex with WDR4 which assists its function in tRNA modification. This complex is necessary for the precise modification of tRNA ensuring accurate protein synthesis and cellular homeostasis. Investigations reveal METTL1's role in cellular processes such as proliferation and stem cell maintenance highlighting its functional importance in cell cycle control and organismal development.

Pathways

METTL1 integrates into significant molecular pathways like mRNA translation and tRNA processing. METTL1 associates with proteins like SAM and FTO within these pathways. The enzyme influences the mTOR signaling pathway which plays a major role in regulating cellular growth and metabolism. Through its methyltransferase activity METTL1 impacts the proper functioning of ribosomes and protein synthesis linking it to the translational control of gene expression.

Research links METTL1 to various cancers including glioblastoma and lung cancer. Aberrant METTL1 expression or activity correlates with tumor progression and malignancy due to its role in altered protein synthesis. METTL1 dysregulation also relates to intellectual disabilities highlighting its importance in brain function. In the context of cancer proteins like mTOR and MYC have been connected to METTL1 by affecting pathways which influence tumor cell survival and proliferation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Catalytic component of METTL1-WDR4 methyltransferase complex that mediates the formation of N(7)-methylguanine in a subset of RNA species, such as tRNAs, mRNAs and microRNAs (miRNAs) (PubMed : 12403464, PubMed : 31031083, PubMed : 31031084, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656, PubMed : 37379838). Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in a large subset of tRNAs that contain the 5'-RAGGU-3' motif within the variable loop (PubMed : 12403464, PubMed : 34352206, PubMed : 34352207, PubMed : 36599982, PubMed : 36599985, PubMed : 37369656). M7G46 interacts with C13-G22 in the D-loop to stabilize tRNA tertiary structure and protect tRNAs from decay (PubMed : 36599982, PubMed : 36599985). Also acts as a methyltransferase for a subset of internal N(7)-methylguanine in mRNAs (PubMed : 31031084, PubMed : 37379838). Internal N(7)-methylguanine methylation of mRNAs in response to stress promotes their relocalization to stress granules, thereby suppressing their translation (PubMed : 31031084, PubMed : 37379838). Also methylates a specific subset of miRNAs, such as let-7 (PubMed : 31031083). N(7)-methylguanine methylation of let-7 miRNA promotes let-7 miRNA processing by disrupting an inhibitory secondary structure within the primary miRNA transcript (pri-miRNA) (PubMed : 31031083). Acts as a regulator of embryonic stem cell self-renewal and differentiation (By similarity).

See full target information METTL1 phospho S27

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308769 PubMed38810124

2024

The m7G Methyltransferase Mettl1 Drives Cardiac Hypertrophy by Regulating SRSF9-Mediated Splicing of NFATc4.

Applications

Unspecified application

Species

Unspecified reactive species

Shuting Yu,ZhiYong Sun,Tiantian Ju,Yingqi Liu,Zhongting Mei,Changhao Wang,Zhezhe Qu,Na Li,Fan Wu,KuiWu Liu,Meixi Lu,Min Huang,Xiaochen Pang,Yingqiong Jia,Ying Li,Yaozhi Zhang,Shunkang Dou,Jianhao Jiang,Xianhui Dong,Chuanhao Huang,Wanhong Li,Yi Zhang,Ye Yuan,Baofeng Yang,Weijie Du

Cell death discovery 8:458 PubMed36396627

2022

METTL1 drives tumor progression of bladder cancer via degrading ATF3 mRNA in an mG-modified miR-760-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Haiyun Xie,Mingchao Wang,Haifeng Yu,Huan Wang,Lifeng Ding,Ruyue Wang,Wenqin Luo,Zeyi Lu,Qiming Zheng,Liangliang Ren,Zhenwei Zhou,Wenjing Su,Liqun Xia,Gonghui Li

Molecular cell 81:3323-3338.e14 PubMed34352207

2021

METTL1-mediated mG modification of Arg-TCT tRNA drives oncogenic transformation.

Applications

Unspecified application

Species

Unspecified reactive species

Esteban A Orellana,Qi Liu,Eliza Yankova,Mehdi Pirouz,Etienne De Braekeleer,Wencai Zhang,Jihoon Lim,Demetrios Aspris,Erdem Sendinc,Dimitrios A Garyfallos,Muxin Gu,Raja Ali,Alejandro Gutierrez,Sigitas Mikutis,Gonçalo J L Bernardes,Eric S Fischer,Allan Bradley,George S Vassiliou,Frank J Slack,Konstantinos Tzelepis,Richard I Gregory

Molecular cell 74:1278-1290.e9 PubMed31031083

2019

METTL1 Promotes let-7 MicroRNA Processing via m7G Methylation.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Pandolfini,Isaia Barbieri,Andrew J Bannister,Alan Hendrick,Byron Andrews,Natalie Webster,Pierre Murat,Pia Mach,Rossella Brandi,Samuel C Robson,Valentina Migliori,Andrej Alendar,Mara d'Onofrio,Shankar Balasubramanian,Tony Kouzarides

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Product promise

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Anti-METTL1 antibody

Western blot - Anti-METTL1 antibody (AB157097)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

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