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AB186002

Anti-METTL13 antibody

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(4 Publications)

Rabbit Polyclonal METTL13 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human METTL13 aa 650 to C-terminus.

View Alternative Names

EEF1AKNMT, FEAT, KIAA0859, CGI-01, METTL13, eEF1A lysine and N-terminal methyltransferase, eEF1A-KNMT, Methyltransferase-like protein 13

2 Images
Immunoprecipitation - Anti-METTL13 antibody (AB186002)
  • IP

Supplier Data

Immunoprecipitation - Anti-METTL13 antibody (AB186002)

Detection of Human METTL13 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab186002 used for IP at 1 μg/ml. For blotting immunoprecipitated METTL13, ab186002 was used at 1 μg/ml.

All lanes:

Immunoprecipitation - Anti-METTL13 antibody (ab186002)

Predicted band size: 79 kDa

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Exposure time: 3min

Western blot - Anti-METTL13 antibody (AB186002)
  • WB

Supplier Data

Western blot - Anti-METTL13 antibody (AB186002)

All lanes:

Western blot - Anti-METTL13 antibody (ab186002) at 0.4 mg/mL

Lane 1:

HeLa lysate at 50 µg

Lane 2:

293T lysate at 50 µg

Lane 3:

Jurkat lysate at 50 µg

Predicted band size: 79 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human METTL13 aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N6R0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL13 also known as METTL13-NAT2 is a methyltransferase enzyme that plays a mechanical role in protein synthesis through methylation. It transfers methyl groups particularly at the N-terminus of elongation factor 1A (eEF1A) which is essential for its activity. The molecular weight of METTL13 is approximately 64 kDa. This enzyme is expressed in various tissues with significant levels observed in the liver heart and skeletal muscle indicating an important role in bodily metabolic processes.
Biological function summary

METTL13 is highly involved in the regulation of protein translation. By methylating the elongation factor eEF1A METTL13 influences protein synthesis at the ribosome ensuring the proper function and fidelity of translation. METTL13 is a part of the broader group of methyltransferases which are enzymes that modify proteins and other biomolecules by adding methyl groups impacting gene expression and cellular processes.

Pathways

METTL13 integrates into translational control and signaling pathways. One of the key pathways including METTL13 is the mTOR signaling pathway which governs cell growth and metabolism through protein synthesis. METTL13 interacts with proteins within this pathway such as eEF2K influencing translation and cellular metabolism. In the context of cell signaling METTL13 contributes to gene expression modulation impacting cellular responses to environmental signals.

METTL13 has been linked to cancer and metabolic disorders. Studies reveal an association between aberrant METTL13 expression and tumor growth suggesting a role in oncogenesis through the modification of protein synthesis and cellular proliferation pathways. Additionally METTL13 might influence metabolic disorders by affecting the proteins involved in cellular metabolic regulation. The interplay between METTL13 and proteins like eEF1A highlights the complexity in disease progression and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed : 30143613, PubMed : 30612740). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed : 30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed : 30143613, PubMed : 30612740). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed : 30612740).
See full target information METTL13
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

World journal of gastrointestinal oncology 17:105933 PubMed40487959

2025

Circular RNA hsa_circRNA_101996 modulates gastric cancer cell proliferation and apoptosis through the miR-577/HMGN5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Lei Wang,Lin Zhang,Qing Shang

Journal of cell communication and signaling 17:121-135 PubMed35925508

2022

METTL13 facilitates cell growth and metastasis in gastric cancer via an eEF1A/HN1L positive feedback circuit.

Applications

Unspecified application

Species

Unspecified reactive species

Qiong Wu,Qingqing Hu,Yanan Hai,Yandong Li,Yong Gao

Journal of translational medicine 19:209 PubMed33985542

2021

METTL13 inhibits progression of clear cell renal cell carcinoma with repression on PI3K/AKT/mTOR/HIF-1α pathway and c-Myc expression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuonan Liu,Tianshui Sun,Chiyuan Piao,Zhe Zhang,Chuize Kong

EBioMedicine 54:102722 PubMed32268273

2020

TRAF4 acts as a fate checkpoint to regulate the adipogenic differentiation of MSCs by activating PKM2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuizhong Cen,Jinteng Li,Zhaopeng Cai,Yiqian Pan,Zehang Sun,Zhaofeng Li,Guiwen Ye,Guan Zheng,Ming Li,Wenjie Liu,Wenhui Yu,Shan Wang,Zhongyu Xie,Peng Wang,Huiyong Shen
View all publications
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