AB186008

Anti-METTL13 antibody - C-terminal

Name: Anti-METTL13 antibody - C-terminal (ab186008) | Abcam
Brand: Abcam Limited
SKU: AB186008
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal METTL13 antibody. C-terminal. Suitable for WB and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human METTL13 aa 600 to C-terminus.

View Alternative Names

EEF1AKNMT, FEAT, KIAA0859, CGI-01, METTL13, eEF1A lysine and N-terminal methyltransferase, eEF1A-KNMT, Methyltransferase-like protein 13

1 Images

Supplier Data

Western blot - Anti-METTL13 antibody - C-terminal (AB186008)

All lanes:

Western blot - Anti-METTL13 antibody - C-terminal (ab186008) at 1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK whole cell lysate at 50 µg

Lane 5:

3T3 whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human METTL13 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N6R0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2500", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab186008 was affinity purified using an epitope specific to METTL13 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL13 also known as METTL13-NAT2 is a methyltransferase enzyme that plays a mechanical role in protein synthesis through methylation. It transfers methyl groups particularly at the N-terminus of elongation factor 1A (eEF1A) which is essential for its activity. The molecular weight of METTL13 is approximately 64 kDa. This enzyme is expressed in various tissues with significant levels observed in the liver heart and skeletal muscle indicating an important role in bodily metabolic processes.

Biological function summary

METTL13 is highly involved in the regulation of protein translation. By methylating the elongation factor eEF1A METTL13 influences protein synthesis at the ribosome ensuring the proper function and fidelity of translation. METTL13 is a part of the broader group of methyltransferases which are enzymes that modify proteins and other biomolecules by adding methyl groups impacting gene expression and cellular processes.

Pathways

METTL13 integrates into translational control and signaling pathways. One of the key pathways including METTL13 is the mTOR signaling pathway which governs cell growth and metabolism through protein synthesis. METTL13 interacts with proteins within this pathway such as eEF2K influencing translation and cellular metabolism. In the context of cell signaling METTL13 contributes to gene expression modulation impacting cellular responses to environmental signals.

METTL13 has been linked to cancer and metabolic disorders. Studies reveal an association between aberrant METTL13 expression and tumor growth suggesting a role in oncogenesis through the modification of protein synthesis and cellular proliferation pathways. Additionally METTL13 might influence metabolic disorders by affecting the proteins involved in cellular metabolic regulation. The interplay between METTL13 and proteins like eEF1A highlights the complexity in disease progression and potential therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed : 30143613, PubMed : 30612740). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed : 30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed : 30143613, PubMed : 30612740). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed : 30612740).

See full target information METTL13

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:3411 PubMed30143613

2018

The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates.

Applications

Unspecified application

Species

Unspecified reactive species

Magnus E Jakobsson,Jędrzej M Małecki,Levon Halabelian,Benedikt S Nilges,Rita Pinto,Srikanth Kudithipudi,Stephanie Munk,Erna Davydova,Fawzi R Zuhairi,Cheryl H Arrowsmith,Albert Jeltsch,Sebastian A Leidel,Jesper V Olsen,Pål Ø Falnes

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-METTL13 antibody - C-terminal

Western blot - Anti-METTL13 antibody - C-terminal (AB186008)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise