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AB229145

Anti-METTL13 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal METTL13 antibody. N-terminal. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human METTL13.

View Alternative Names

EEF1AKNMT, FEAT, KIAA0859, CGI-01, METTL13, eEF1A lysine and N-terminal methyltransferase, eEF1A-KNMT, Methyltransferase-like protein 13

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL13 antibody - N-terminal (AB229145)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL13 antibody - N-terminal (AB229145)

Paraffin-embedded human ovarian carcinoma tissue stained for METTL13 using ab229145 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-METTL13 antibody - N-terminal (AB229145)
  • WB

Supplier Data

Western blot - Anti-METTL13 antibody - N-terminal (AB229145)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-METTL13 antibody - N-terminal (ab229145) at 1/1000 dilution

All lanes:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 79 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human METTL13. The exact immunogen used to generate this antibody is proprietary information.

Q8N6R0

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL13 also known as METTL13-NAT2 is a methyltransferase enzyme that plays a mechanical role in protein synthesis through methylation. It transfers methyl groups particularly at the N-terminus of elongation factor 1A (eEF1A) which is essential for its activity. The molecular weight of METTL13 is approximately 64 kDa. This enzyme is expressed in various tissues with significant levels observed in the liver heart and skeletal muscle indicating an important role in bodily metabolic processes.
Biological function summary

METTL13 is highly involved in the regulation of protein translation. By methylating the elongation factor eEF1A METTL13 influences protein synthesis at the ribosome ensuring the proper function and fidelity of translation. METTL13 is a part of the broader group of methyltransferases which are enzymes that modify proteins and other biomolecules by adding methyl groups impacting gene expression and cellular processes.

Pathways

METTL13 integrates into translational control and signaling pathways. One of the key pathways including METTL13 is the mTOR signaling pathway which governs cell growth and metabolism through protein synthesis. METTL13 interacts with proteins within this pathway such as eEF2K influencing translation and cellular metabolism. In the context of cell signaling METTL13 contributes to gene expression modulation impacting cellular responses to environmental signals.

METTL13 has been linked to cancer and metabolic disorders. Studies reveal an association between aberrant METTL13 expression and tumor growth suggesting a role in oncogenesis through the modification of protein synthesis and cellular proliferation pathways. Additionally METTL13 might influence metabolic disorders by affecting the proteins involved in cellular metabolic regulation. The interplay between METTL13 and proteins like eEF1A highlights the complexity in disease progression and potential therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Dual methyltransferase that catalyzes methylation of elongation factor 1-alpha (EEF1A1 and EEF1A2) at two different positions, and is therefore involved in the regulation of mRNA translation (PubMed : 30143613, PubMed : 30612740). Via its C-terminus, methylates EEF1A1 and EEF1A2 at the N-terminal residue 'Gly-2' (PubMed : 30143613). Via its N-terminus dimethylates EEF1A1 and EEF1A2 at residue 'Lys-55' (PubMed : 30143613, PubMed : 30612740). Has no activity towards core histones H2A, H2B, H3 and H4 (PubMed : 30612740).
See full target information METTL13
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncology letters 18:4421-4428 PubMed31611951

2019

STAMBPL1 knockdown has antitumour effects on gastric cancer biological activities.

Applications

Unspecified application

Species

Unspecified reactive species

Da-Jun Yu,Jun Qian,Xin Jin,Jing Li,Chen-Xu Guo,Xi-Cheng Yue
View all publications
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Product promise

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