Rabbit Polyclonal METTL14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human METTL14 aa 1-100.
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- Journal of cellular physiology 238:2361-23722023Mettl14-mediated m A modification regulates the abnormal differentiation of small intestinal epithelial stem cells in diabetic state.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTi-Dong Shan,Yue Han,Ming-Quan Song,Li ChenPubMed 37565545
- Journal of neurochemistry 166:328-3452023Astragaloside IV alleviates neuronal ferroptosis in ischemic stroke by regulating fat mass and obesity-associated-N6-methyladenosine-acyl-CoA synthetase long-chain family member 4 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhenglong Jin,Wenying Gao,Fu Guo,Shaojun Liao,Mingzhe Hu,Tao Yu,Shangzhen Yu,Qing ShiPubMed 37300304
- iScience 26:1062152023mA demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRi-Feng Gao,Kun Yang,Ya-Nan Qu,Xiang Wei,Jia-Ran Shi,Chun-Yu Lv,Yong-Chao Zhao,Xiao-Lei Sun,Ying-Jia Xu,Yi-Qing YangPubMed 36876119
- Cell reports 40:1111072022Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRobert J J Jansens,Ruth Verhamme,Aashiq H Mirza,Anthony Olarerin-George,Cliff Van Waesberghe,Samie R Jaffrey,Herman W FavoreelPubMed 35858564
- Journal of oncology 2022:27569862022LncRNA UCA1 Promotes the Progression of AML by Upregulating the Expression of CXCR4 and CYP1B1 by Affecting the Stability of METTL14.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiajia Li,Zhongyu Li,Xue Bai,Xiaofeng Chen,Meng Wang,Yanping Wu,Haotian WuPubMed 35178087
- Journal of translational medicine 20:512022piRNA-14633 promotes cervical cancer cell malignancy in a METTL14-dependent m6A RNA methylation manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQi Xie,Zhen Li,Xiao Luo,Dan Wang,Yao Zhou,Jingge Zhao,Suhua Gao,Yongguang Yang,Wanying Fu,Lingfei Kong,Tingyi SunPubMed 35093098
- Bioengineered 13:1858-18712022N(6)-adenosine-methyltransferase-14 promotes glioma tumorigenesis by repressing argininosuccinate synthase 1 expression in an m6A-dependent manner.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYou-Qing Miao,Wei Chen,Jianfeng Zhou,Qiyang Shen,Ying Sun,Tao Li,Sheng-Chan WangPubMed 35012429
- Cell death discovery 8:152022METTL14 promotes apoptosis of spinal cord neurons by inducing EEF1A2 m6A methylation in spinal cord injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGang Gao,Yufen Duan,Feng Chang,Ting Zhang,Xinhu Huang,Chen YuPubMed 35013140
- Molecular medicine (Cambridge, Mass.) 27:1062021METTL14 promotes glomerular endothelial cell injury and diabetic nephropathy via m6A modification of α-klotho.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManna Li,Le Deng,Gaosi XuPubMed 34503454
- Frontiers in cell and developmental biology 9:6424372021N6-Methyladenosine Induced miR-34a-5p Promotes TNF-α-Induced Nucleus Pulposus Cell Senescence by Targeting SIRT1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Zhu,Bao Sun,Liang Zhu,Guoyou Zou,Qiang ShenPubMed 33763423
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human METTL14 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCE5
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Cow | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted |
| Xenopus tropicalis | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes 50 - 100 μL / mg of lysate. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Xenopus laevis, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/1000 | Notes - |
Species Human | Dilution info 1/1000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Xenopus laevis, Orangutan, Xenopus tropicalis | Dilution info - | Notes - |
Associated Products
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Target data
Function
The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27281194, PubMed:27373337, PubMed:29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27281194, PubMed:27373337, PubMed:27627798, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).
Alternative names
KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14
Recommended products
Rabbit Polyclonal METTL14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human METTL14 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human METTL14 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9HCE5
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
ab252562 was affinity purified using an epitope specific to METTL14 immobilized on a solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
METTL14 also known in scientific circles as Methyltransferase-like 14 plays an important mechanical role in RNA methylation. This protein with a molecular weight of approximately 58 kDa functions as part of the m6A methyltransferase complex. METTL14 is mainly expressed in the nucleus of cells where it helps catalyze the formation of N6-methyladenosine (m6A) on RNA which is a significant epigenetic modification.
- Biological function summary
METTL14 contributes greatly to the modification of messenger RNA (mRNA) through its involvement in the m6A methyltransferase complex alongside METTL3. This protein complex operates to methylate adenine residues in mRNA influencing mRNA stability splicing translation and eventually degradation. METTL14's function in this complex is more regulatory stabilizing the interaction between METTL3 and its RNA substrate modulating the final methylation outcomes.
- Pathways
METTL14's methylation activity greatly impacts gene expression through the mRNA processing and metabolism pathways. The m6A modification where METTL14 acts is important in mRNA fate and participates significantly in pathways controlling cell differentiation and proliferation. Researchers link it to major proteins like YTH domain family proteins which recognize and bind to m6A-modified RNA propagating the downstream effects of mRNA processing.
- Associated diseases and disorders
Researchers have drawn significant links between METTL14 and various forms of cancer including leukemia. Altered METTL14 expression can dysregulate mRNA metabolism leading to tumorigenesis. Additionally METTL14 has been implicated in neurodevelopmental disorders due to its role in brain development and function. Variations in METTL14 expression or activity may influence these diseases through its interactions with METTL3 and other complex components that can affect gene expression patterns important for normal cell function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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2 product images
Western blot - Anti-METTL14 antibody (ab252562)
Lysates prepared in NETN buffer.
All lanes: Western blot - Anti-METTL14 antibody (ab252562) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg
Lane 2: RKO whole cell lysate at 15 µg
Lane 3: GaMG whole cell lysate at 15 µg
Lane 4: TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Exposure time: 10s
Immunoprecipitation - Anti-METTL14 antibody (ab252562)
METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252562 at 100 μL per reaction. Western blot was performed from the immunoprecipitate using ab252562 at 1/1000 dilution.
Lane 1: ab252562 IP in Jurkat whole cell lysate.
Lane 2: Anti-METTL14 antibody ab264408 instead of ab252562 in Jurkat whole cell lysate.
Lane 3: Control IgG IP in Jurkat whole cell lysate.Detection: Chemiluminescence with exposure time of 3 seconds.
Lysates prepared in NETN buffer.
Lane 1: Immunoprecipitation - Anti-METTL14 antibody (ab252562)
Lane 1: Immunoprecipitation - Anti-METTL14 antibody (Anti-METTL14 antibody ab264408)
Predicted band size: 52 kDa
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