AB252562

Anti-METTL14 antibody

Name: Anti-METTL14 antibody (ab252562) | Abcam
Brand: Abcam Limited
SKU: AB252562
Price: 525 USD
Availability: InStock

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(23 Publications)

Rabbit Polyclonal METTL14 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 23 publications. Immunogen corresponding to Synthetic Peptide within Human METTL14 aa 1-100.

View Alternative Names

KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14

2 Images

Supplier Data

Immunoprecipitation - Anti-METTL14 antibody (AB252562)

METTL14 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab252562 at 100 μL per reaction. Western blot was performed from the immunoprecipitate using ab252562 at 1/1000 dilution.

Lane 1 : ab252562 IP in Jurkat whole cell lysate.
Lane 2 : ab264408 instead of ab252562 in Jurkat whole cell lysate.
Lane 3 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 seconds.

Lysates prepared in NETN buffer.

Lane 1:

Immunoprecipitation - Anti-METTL14 antibody (ab252562)

Lane 1:

Immunoprecipitation - Anti-METTL14 antibody (<a href='/en-us/products/primary-antibodies/mettl14-antibody-ab264408'>ab264408</a>)

Predicted band size: 52 kDa

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Supplier Data

Western blot - Anti-METTL14 antibody (AB252562)

Lysates prepared in NETN buffer.

All lanes:

Western blot - Anti-METTL14 antibody (ab252562) at 1/1000 dilution

Lane 1:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 15 µg

Lane 2:

RKO whole cell lysate at 15 µg

Lane 3:

GaMG whole cell lysate at 15 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 15 µg

Predicted band size: 52 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human METTL14 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9HCE5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "50 - 100 μL / mg of lysate.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab252562 was affinity purified using an epitope specific to METTL14 immobilized on a solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL14 also known in scientific circles as Methyltransferase-like 14 plays an important mechanical role in RNA methylation. This protein with a molecular weight of approximately 58 kDa functions as part of the m6A methyltransferase complex. METTL14 is mainly expressed in the nucleus of cells where it helps catalyze the formation of N6-methyladenosine (m6A) on RNA which is a significant epigenetic modification.

Biological function summary

METTL14 contributes greatly to the modification of messenger RNA (mRNA) through its involvement in the m6A methyltransferase complex alongside METTL3. This protein complex operates to methylate adenine residues in mRNA influencing mRNA stability splicing translation and eventually degradation. METTL14's function in this complex is more regulatory stabilizing the interaction between METTL3 and its RNA substrate modulating the final methylation outcomes.

Pathways

METTL14's methylation activity greatly impacts gene expression through the mRNA processing and metabolism pathways. The m6A modification where METTL14 acts is important in mRNA fate and participates significantly in pathways controlling cell differentiation and proliferation. Researchers link it to major proteins like YTH domain family proteins which recognize and bind to m6A-modified RNA propagating the downstream effects of mRNA processing.

Researchers have drawn significant links between METTL14 and various forms of cancer including leukemia. Altered METTL14 expression can dysregulate mRNA metabolism leading to tumorigenesis. Additionally METTL14 has been implicated in neurodevelopmental disorders due to its role in brain development and function. Variations in METTL14 expression or activity may influence these diseases through its interactions with METTL3 and other complex components that can affect gene expression patterns important for normal cell function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671, PubMed : 27281194, PubMed : 27373337, PubMed : 29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed : 27281194, PubMed : 27373337, PubMed : 27627798, PubMed : 29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis : m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

See full target information METTL14

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

NAR cancer 7:zcaf037 PubMed41064808

2025

Co-expression of CD30 and SLFN11 serves as a dual biomarker for the treatment of cutaneous T-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Takatoshi Shimauchi,Junko Murai,Manami Iwasaki,Pawit Phadungsaksawasdi,Toshiyuki Ojima,Kazuyasu Fujii,Tetsuya Honda

Biomolecules & biomedicine 25:682-692 PubMed39319864

2024

Regulatory role and molecular mechanism of METTL14 in vascular endothelial cell injury in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Huafang Wei,Lin Liang,Chengwen Song,Ming Tong,Xiang Xu

Daru : journal of Faculty of Pharmacy, Tehran University of Medical Sciences 32:11-24 PubMed37926762

2023

Chidamide inhibits cell glycolysis in acute myeloid leukemia by decreasing N6-methyladenosine-related GNAS-AS1.

Applications

Unspecified application

Species

Unspecified reactive species

Changmei Hu,Xiao Fu,Shujun Li,Cong Chen,Xielan Zhao,Jie Peng

Journal of cellular physiology 238:2361-2372 PubMed37565545

2023

Mettl14-mediated m A modification regulates the abnormal differentiation of small intestinal epithelial stem cells in diabetic state.

Applications

Unspecified application

Species

Unspecified reactive species

Ti-Dong Shan,Yue Han,Ming-Quan Song,Li Chen

Journal of neurochemistry 166:328-345 PubMed37300304

2023

Astragaloside IV alleviates neuronal ferroptosis in ischemic stroke by regulating fat mass and obesity-associated-N6-methyladenosine-acyl-CoA synthetase long-chain family member 4 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenglong Jin,Wenying Gao,Fu Guo,Shaojun Liao,Mingzhe Hu,Tao Yu,Shangzhen Yu,Qing Shi

Journal of cell communication and signaling 17:897-914 PubMed36947363

2023

METTL3-METTL14 complex induces necroptosis and inflammation of vascular smooth muscle cells via promoting N6 methyladenosine mRNA methylation of receptor-interacting protein 3 in abdominal aortic aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Li,Dongbin Zhang,Shuiting Zhai,Huilin Wu,Hongzhi Liu

iScience 26:106215 PubMed36876119

2023

mA demethylase ALKBH5 attenuates doxorubicin-induced cardiotoxicity via posttranscriptional stabilization of Rasal3.

Applications

Unspecified application

Species

Unspecified reactive species

Ri-Feng Gao,Kun Yang,Ya-Nan Qu,Xiang Wei,Jia-Ran Shi,Chun-Yu Lv,Yong-Chao Zhao,Xiao-Lei Sun,Ying-Jia Xu,Yi-Qing Yang

iScience 25:105620 PubMed36465115

2022

TWIK-related acid-sensitive K channel 2 promotes renal fibrosis by inducing cell-cycle arrest.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Zhang,Jing Chen,Yufei Lu,Yan Yang,Weize Chen,Bo Shen,Jiachang Hu,Ping Jia,Sujuan Xu,Yiqin Shi,Yichun Ning,Jialin Wang,Yi Fang,Shuan Zhao,Yang Li,Yan Dai,Xiaoyan Zhang,Meng Xiang,Yang Tian,Zhichao Liu,Nana Song,Xiaoqiang Ding

Journal of animal science and biotechnology 13:146 PubMed36457054

2022

Coordinated transcriptional and post-transcriptional epigenetic regulation during skeletal muscle development and growth in pigs.

Applications

Unspecified application

Species

Unspecified reactive species

Du Zhang,Shumei Wu,Xinxin Zhang,Shuqiang Ren,Zhonglin Tang,Fei Gao

Clinical immunology (Orlando, Fla.) 245:109176 PubMed36368640

2022

Matr3 reshapes m6A modification complex to alleviate macrophage inflammation during atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Zewei Sun,Wenjing Chen,Zhen Wang,Shuai Wang,Jie Zan,Liangrong Zheng,Wenting Zhao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-METTL14 antibody

Immunoprecipitation - Anti-METTL14 antibody (AB252562)

Western blot - Anti-METTL14 antibody (AB252562)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

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