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Rabbit Recombinant Monoclonal METTL14 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

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Images

Western blot - Anti-METTL14 antibody [EPR27234-62] (AB309096), expandable thumbnail
  • Western blot - Anti-METTL14 antibody [EPR27234-62] (AB309096), expandable thumbnail
  • Western blot - Anti-METTL14 antibody [EPR27234-62] (AB309096), expandable thumbnail
  • Immunoprecipitation - Anti-METTL14 antibody [EPR27234-62] (AB309096), expandable thumbnail
  • Western blot - Anti-METTL14 antibody [EPR27234-62] (AB309096), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed:24316715, PubMed:24407421, PubMed:25719671, PubMed:27281194, PubMed:27373337, PubMed:29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed:27281194, PubMed:27373337, PubMed:27627798, PubMed:29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).

Alternative names

KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14

Recommended products

Rabbit Recombinant Monoclonal METTL14 antibody. Suitable for WB, IP and reacts with Human, Mouse, Rat samples. Cited in 11 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR27234-62
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

METTL14 also known in scientific circles as Methyltransferase-like 14 plays an important mechanical role in RNA methylation. This protein with a molecular weight of approximately 58 kDa functions as part of the m6A methyltransferase complex. METTL14 is mainly expressed in the nucleus of cells where it helps catalyze the formation of N6-methyladenosine (m6A) on RNA which is a significant epigenetic modification.

Biological function summary

METTL14 contributes greatly to the modification of messenger RNA (mRNA) through its involvement in the m6A methyltransferase complex alongside METTL3. This protein complex operates to methylate adenine residues in mRNA influencing mRNA stability splicing translation and eventually degradation. METTL14's function in this complex is more regulatory stabilizing the interaction between METTL3 and its RNA substrate modulating the final methylation outcomes.

Pathways

METTL14's methylation activity greatly impacts gene expression through the mRNA processing and metabolism pathways. The m6A modification where METTL14 acts is important in mRNA fate and participates significantly in pathways controlling cell differentiation and proliferation. Researchers link it to major proteins like YTH domain family proteins which recognize and bind to m6A-modified RNA propagating the downstream effects of mRNA processing.

Associated diseases and disorders

Researchers have drawn significant links between METTL14 and various forms of cancer including leukemia. Altered METTL14 expression can dysregulate mRNA metabolism leading to tumorigenesis. Additionally METTL14 has been implicated in neurodevelopmental disorders due to its role in brain development and function. Variations in METTL14 expression or activity may influence these diseases through its interactions with METTL3 and other complex components that can affect gene expression patterns important for normal cell function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    Exposure time: Anti-METTL14 antibody [EPR22666-22] ab300104: 3 seconds; ab309096: 40 seconds.

    Anti-METTL14 antibody [EPR22666-22] ab300104 is highly sensitive but unfortunately detects a nonspecific band near the METTL14 target band.

    Lanes 1 - 7: Western blot - Anti-METTL14 antibody [EPR22666-22] (Anti-METTL14 antibody [EPR22666-22] ab300104) at 1/1000 dilution

    Lanes 8 - 14: Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/1000 dilution

    Lanes 1 and 8: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lanes 2 and 9: NCCIT (human pluripotent embryonic carcinoma epithelial cell) whole cell lysate at 20 µg

    Lanes 3 and 10: 293T (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lanes 4 and 11: Human testis tissue lysate at 20 µg

    Lanes 5 and 12: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lanes 6 and 13: Mouse testis at 20 µg

    Lanes 7 and 14: Mouse lung at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Observed band size: 60 kDa

  • Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

    All lanes: Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/1000 dilution

    Lane 1: Human lung tissue lysate at 20 µg

    Lane 2: Human colon tissue lysate at 20 µg

    Lane 3: Human colon cancer tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Observed band size: 60 kDa

    Exposure time: 180s

  • Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    The identity of the bands between 100 kDa and 300 kDa are unknown.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.

    All lanes: Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeti METTL14 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 60 kDa

    Exposure time: 180s

  • Immunoprecipitation - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Immunoprecipitation - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    METTL14 was immunoprecipitated from 0.35 mg Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab309096 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab309096 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
    Lane 1: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 2: ab309096 IP in Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate
    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab309096 in Raji whole cell lysate
    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/30 dilution

    All lanes: Raji (human Burkitt's lymphoma B lymphocyte) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Observed band size: 60 kDa

    Exposure time: 41s

  • Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.
    The identity of the bands between 150 kDa and 300 kDa are unknown.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: 48 seconds

    All lanes: Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 60 kDa

    Exposure time: 48s

  • Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096), expandable thumbnail

    Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST
    The identity of the bands between 150 kDa and 300 kDa are unknown.
    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
    Exposure time: Lane 1: 48 seconds, lanes 2-3: 180 seconds.

    All lanes: Western blot - Anti-METTL14 antibody [EPR27234-62] (ab309096) at 1/1000 dilution

    Lane 1: Mouse testis tissue lysate at 20 µg

    Lane 2: Rat testis tissue lysate at 20 µg

    Lane 3: Mouse lu tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 60 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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