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AB327393

Anti-METTL14 antibody [EPR28557-25] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal METTL14 antibody. Carrier free. Suitable for sELISA and reacts with Human, Mouse samples.

View Alternative Names

KIAA1627, METTL14, N(6)-adenosine-methyltransferase non-catalytic subunit METTL14, Methyltransferase-like protein 14, hMETTL14

2 Images
Sandwich ELISA - Anti-METTL14 antibody [EPR28557-25] - BSA and Azide free (Detector) (AB327393)
  • sELISA

Lab

Sandwich ELISA - Anti-METTL14 antibody [EPR28557-25] - BSA and Azide free (Detector) (AB327393)

Interpolated concentration of native METTL14 was measured in duplicate at different sample concentrations. Undiluted sample is as follows : A549 cell extract (0.25 mg/mL), SK-MES-1 cell extract (1 mg/ml), and C2C12 cell extract (0.25 mg/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in 1% bovine serum albumin (BSA). We do not guarantee same sensitivity level due to biological variation.

Sandwich ELISA - Anti-METTL14 antibody [EPR28557-25] - BSA and Azide free (Detector) (AB327393)
  • sELISA

Lab

Sandwich ELISA - Anti-METTL14 antibody [EPR28557-25] - BSA and Azide free (Detector) (AB327393)

Sandwich ELISA using Human METTL14 immunogen protein standard curve with the capture ab327392 dilution at 2 ug/mL and detector antibody ab327393 dilution at 0.5 ug/mL. The background-subtracted absorbance data are presented as mean +/- standard deviation.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR28557-25

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>This clone is specific for METTL14</p>

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab327393 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human, Mouse METTL14. The recommended pair for sandwich ELISA is:

Capture: ab327392, Human, Mouse METTL14 Capture Antibody (unconjugated)

Detector: ab327393, Human, Mouse METTL14 Detector Antibody (unconjugated)

The reference range value is 39.06-2,500 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N(6) position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671, PubMed : 27281194, PubMed : 27373337, PubMed : 29348140). In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core (PubMed : 27281194, PubMed : 27373337, PubMed : 27627798, PubMed : 29348140). N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing (PubMed : 24316715, PubMed : 24407421, PubMed : 25719671). M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity). In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity). M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity). M6A also regulates cortical neurogenesis : m6A methylation of transcripts related to transcription factors, neural stem cells, the cell cycle and neuronal differentiation during brain development promotes their destabilization and decay, promoting differentiation of radial glial cells (By similarity).
See full target information METTL14
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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