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AB307820

Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free

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Rabbit Recombinant Monoclonal METTL15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

View Alternative Names

METT5D1, METTL15, 12S rRNA N(4)-cytidine methyltransferase METTL15, 12S rRNA m4C methyltransferase, Methyltransferase 5 domain-containing protein 1, Methyltransferase-like protein 15

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling METTL15 with ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human tonsil. The section was incubated with ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling METTL15 with ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human colon cancer. The section was incubated with ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling METTL15 with ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human colon. The section was incubated with ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • WB

Supplier Data

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180 seconds

All lanes:

Western blot - Anti-METTL15 antibody [EPR25213-46] (<a href='/en-us/products/primary-antibodies/mettl15-antibody-epr25213-46-ab307819'>ab307819</a>) at 1/1000 dilution

Lane 1:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Observed band size: 46 kDa

false

Exposure time: 180s

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • WB

Supplier Data

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200,000 dilution. Exposure time : 26 seconds

All lanes:

Western blot - Anti-METTL15 antibody [EPR25213-46] (<a href='/en-us/products/primary-antibodies/mettl15-antibody-epr25213-46-ab307819'>ab307819</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HeLa transfected with siRNA specifically targeting METTL15 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot at 1/200000 dilution

Observed band size: 46 kDa

false

Exposure time: 26s

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)
  • WB

Supplier Data

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820)

This data was developed using ab307819, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 180 seconds

All lanes:

Western blot - Anti-METTL15 antibody [EPR25213-46] (<a href='/en-us/products/primary-antibodies/mettl15-antibody-epr25213-46-ab307819'>ab307819</a>) at 1/1000 dilution

Lane 1:

Human colon tissue lysate at 20 µg

Lane 2:

Human tonsil tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 46 kDa

false

Exposure time: 180s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25213-46

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL15 also known as methyltransferase-like protein 15 is a methyltransferase enzyme with a molecular mass of approximately 50 kDa. This enzyme catalyzes the transfer of methyl groups and is important in the modulation of RNA function. It is highly expressed in human tissues particularly in the liver and brain indicating its involvement in vital cellular processes. METTL15 is responsible for methylation of adenine residues in ribosomal RNA (rRNA) which is important for ribosome function and maturation.
Biological function summary

METTL15 influences gene expression and translation fidelity by modifying rRNA within the 60S subunit of ribosomes. Through its action METTL15 helps stabilize ribosomal structure assisting in accurate protein synthesis. While METTL15 acts independently its functions are interconnected with other methyltransferases involved in RNA and DNA modification. The precise control of ribosomal methylation is vital for cellular homeostasis and protein synthesis efficiency.

Pathways

METTL15 contributes significantly to ribosome biogenesis and protein translation pathways. It influences the ribosome assembly process integrating into pathways critical for cell growth and proliferation. METTL15's functions relate closely to those of proteins like METTL3 and METTL14 which also partake in RNA methylation processes. Together these proteins interact in pathways that regulate gene expression and cellular response to growth signals.

METTL15 has been implicated in cancer and neurodevelopmental disorders. Altered expression of METTL15 can disrupt normal ribosomal function contributing to tumorigenesis and affecting neuronal development. Its role in cancer is often discussed alongside METTL3 as both are involved in RNA methylation processes linking to oncogenesis. Understanding METTL15's activity in these conditions could help develop targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA (PubMed : 31665743, PubMed : 32371392). Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits (PubMed : 31665743, PubMed : 32371392).
See full target information METTL15
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Product promise

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