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Rabbit Recombinant Monoclonal METTL15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820), expandable thumbnail
  • Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820), expandable thumbnail
  • Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (AB307820), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

N4-methylcytidine (m4C) methyltransferase responsible for the methylation of position C839 in mitochondrial 12S rRNA (PubMed:31665743, PubMed:32371392). Involved in the stabilization of 12S rRNA folding, therefore facilitating the assembly of the mitochondrial small ribosomal subunits (PubMed:31665743, PubMed:32371392).

Alternative names

METT5D1, METTL15, 12S rRNA N(4)-cytidine methyltransferase METTL15, 12S rRNA m4C methyltransferase, Methyltransferase 5 domain-containing protein 1, Methyltransferase-like protein 15

Recommended products

Rabbit Recombinant Monoclonal METTL15 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR25213-46
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

METTL15 also known as methyltransferase-like protein 15 is a methyltransferase enzyme with a molecular mass of approximately 50 kDa. This enzyme catalyzes the transfer of methyl groups and is important in the modulation of RNA function. It is highly expressed in human tissues particularly in the liver and brain indicating its involvement in vital cellular processes. METTL15 is responsible for methylation of adenine residues in ribosomal RNA (rRNA) which is important for ribosome function and maturation.

Biological function summary

METTL15 influences gene expression and translation fidelity by modifying rRNA within the 60S subunit of ribosomes. Through its action METTL15 helps stabilize ribosomal structure assisting in accurate protein synthesis. While METTL15 acts independently its functions are interconnected with other methyltransferases involved in RNA and DNA modification. The precise control of ribosomal methylation is vital for cellular homeostasis and protein synthesis efficiency.

Pathways

METTL15 contributes significantly to ribosome biogenesis and protein translation pathways. It influences the ribosome assembly process integrating into pathways critical for cell growth and proliferation. METTL15's functions relate closely to those of proteins like METTL3 and METTL14 which also partake in RNA methylation processes. Together these proteins interact in pathways that regulate gene expression and cellular response to growth signals.

Associated diseases and disorders

METTL15 has been implicated in cancer and neurodevelopmental disorders. Altered expression of METTL15 can disrupt normal ribosomal function contributing to tumorigenesis and affecting neuronal development. Its role in cancer is often discussed alongside METTL3 as both are involved in RNA methylation processes linking to oncogenesis. Understanding METTL15's activity in these conditions could help develop targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-METTL15 antibody [EPR25213-46] (Anti-METTL15 antibody [EPR25213-46] ab307819) at 1/1000 dilution

    Lane 1: Human colon tissue lysate at 20 µg

    Lane 2: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Western blot at 1/2000 dilution

    Observed band size: 46 kDa

    Exposure time: 180s

  • Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 180 seconds

    All lanes: Western blot - Anti-METTL15 antibody [EPR25213-46] (Anti-METTL15 antibody [EPR25213-46] ab307819) at 1/1000 dilution

    Lane 1: A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Observed band size: 46 kDa

    Exposure time: 180s

  • Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Western blot - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200,000 dilution.

    Exposure time: 26 seconds

    All lanes: Western blot - Anti-METTL15 antibody [EPR25213-46] (Anti-METTL15 antibody [EPR25213-46] ab307819) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

    Lane 2: HeLa transfected with siRNA specifically targeting METTL15 whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot at 1/200000 dilution

    Observed band size: 46 kDa

    Exposure time: 26s

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling METTL15 with Anti-METTL15 antibody [EPR25213-46] ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human colon cancer. The section was incubated with Anti-METTL15 antibody [EPR25213-46] ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling METTL15 with Anti-METTL15 antibody [EPR25213-46] ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human tonsil. The section was incubated with Anti-METTL15 antibody [EPR25213-46] ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL15 antibody [EPR25213-46] - BSA and Azide free (ab307820)

    This data was developed using Anti-METTL15 antibody [EPR25213-46] ab307819, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling METTL15 with Anti-METTL15 antibody [EPR25213-46] ab307819 at 1/50 (9.08 ug/ml) followed by a LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution. Positive staining on human colon. The section was incubated with Anti-METTL15 antibody [EPR25213-46] ab307819 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is LeicaDS9800 (Bond™ Polymer Refine Detection) at Ready to use dilution.

    Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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