Anti-METTL16 antibody

• WB

Supplier Data

Western blot - Anti-METTL16 antibody (AB186012)

All lanes:

Western blot - Anti-METTL16 antibody (ab186012) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

293T whole cell lysate at 50 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

TCMK-1 whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 64 kDa

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Exposure time: 3min

• WB

CiteAb

Western blot - Anti-METTL16 antibody (AB186012)

METTL16 western blot using anti-METTL16 antibody ab186012. Publication image and figure legend from Jabs, S., Biton, A., et al., 2020, Nat Commun, PubMed 32165618.

ab186012 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186012 please see the product overview.

Differential expression of Mettl16 and Mat2a in mouse cecum.a Western blot analysis of Mettl16 expression from ceca of CONV, GF, ex-GF, abx, vanco, Am, and Lp mice. Actin served as loading control. The membrane and thus the actin loading control for the blot displaying samples from ex-GF, Am, and Lp mice is identical to the western blot in Supplementary Fig. 4a. b Quantification using six different western blots; CONV n = 20, ex-GF n = 6; GF n = 13; abx n = 9; vanco n = 8; Am n = 9; Lp n = 9; Mettl16/Actin ratio was normalized to ratio in CONV mice in order to compare protein expression across multiple Western Blots. Ordinary one-way ANOVA was performed. *p-value < 0.05; p-values (compared to CONV) : ex-GF : 0.9518; GF : 0.0255; abx : 0.0431; vanco : 0.1750; Am : 0.1783; Lp : 0.2728) (Holm–Sidak's multiple comparisons test). c Representation of two m6A peaks (mean of read per million normalized coverage (RPM) in detected methylation peaks) from anti-m6A immunoprecipitates and input in the 3'UTR of the Mat2a transcript in cecum. The peaks designated a and b were visualized for the indicated mouse models using IGV; d Quantification of indicated Mat2a peaks (a, b from b) as -log2 normalized read counts. Ordinary one-way ANOVA for multiple comparisons was performed. CONV (n = 15), GF (n = 12); ex-GF (n = 4); abx (n = 9); vanco (n = 8); Am (n = 11), Lp (n = 3); two independent sequencing experiments; ap-values (all compared to CONV) : ex-GF : 0.6458; GF : 0.0039; abx : 0.0572; vanco : 0.6458; Am : 0.6458; Lp : 0.4695; bp-values (all compared to CONV) : ex-GF : 0.889955; GF : 0.000323; abx : 0.003604; vanco : 0.889955; Am : 0.00000000000028; Lp : 0.018424; e anti-m6A-IP and qRT for Mat2a transcript in CONV (n = 11), ex-GF(n = 5) and GF (n = 8) cecal RNA; IgG-IP (n = 4) served as control for unspecific binding; ordinary one-way ANOVA was performed; *p-value < 0.05; ***p-value < 0.005; p-values (compared to CONV) : ex-GF : 0.6897; GF : 0.0127; IgG : 0.0029; f Western Blot analysis of Mat2a expression in CONV, GF, ex-GF, abx, vanco, Am, and Lp cecum. Actin served as loading control. Asterisk marks an unspecific band. g Quantification of Mat2a expression in CONV, GF, ex-GF, abx, vanco, Am, and Lp cecum. Quantification was performed using at least three different western blots for each condition; CONV n = 15, ex-GF n = 6; GF n = 12; abx n = 12; vanco n = 12; Am n = 9; Lp n = 9; Mat2a/Actin ratio was normalized to ratio in CONV mice in order to compare protein expression across multiple western blots; ordinary one-way ANOVA was performed. ***<0.0001, **p-value < 0.005 (Holm–Sidak's multiple comparisons test); p-values (all compared to CONV) : ex-GF : 0.157; GF : 0.00000019; abx : 0.0002; vanco : 0.0019; Am : 0.1111; Lp : 0.154; data are presented as mean values ±SEM throughout the figure; details for statistical analysis and original data for a, b, d–g are given in the source data file.

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