Rabbit Polyclonal METTL16 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human METTL16 aa 350-450.
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- Oxidative medicine and cellular longevity 2022:40362742022Oxidative Stress Aggravates Apoptosis of Nucleus Pulposus Cells through mA Modification of MAT2A Pre-mRNA by METTL16.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng-Bo Chen,Gui-Xun Shi,Tao Liu,Bo Li,Sheng-Dan Jiang,Xin-Feng Zheng,Lei-Sheng JiangPubMed 35069973
- Cell 184:3125-3142.e252021Splice site mA methylation prevents binding of U2AF35 to inhibit RNA splicing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMateusz Mendel,Kamila Delaney,Radha Raman Pandey,Kuan-Ming Chen,Joanna M Wenda,Cathrine Broberg Vågbø,Florian A Steiner,David Homolka,Ramesh S PillaiPubMed 33930289
- Nature communications 11:13442020Impact of the gut microbiota on the mA epitranscriptome of mouse cecum and liver.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSabrina Jabs et. al.PubMed 32165618
- Molecular cell 71:986-1000.e112018Methylation of Structured RNA by the mA Writer METTL16 Is Essential for Mouse Embryonic Development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMateusz Mendel et. al.PubMed 30197299
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human METTL16 aa 350-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86W50
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
| Rat | Predicted |
| Cat | Predicted |
| Chimpanzee | Predicted |
| Chinese hamster | Predicted |
| Dog | Predicted |
| Gorilla | Predicted |
| Guinea pig | Predicted |
| Horse | Predicted |
| Orangutan | Predicted |
| Rabbit | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Species Mouse | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Chinese hamster, Orangutan | Dilution info - | Notes - |
Associated Products
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2 products for Alternative Product
Target data
Function
RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed:28525753, PubMed:30197297, PubMed:30197299, PubMed:33428944, PubMed:33930289). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed:28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs: requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed:28525753, PubMed:30197297, PubMed:30197299). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts: in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed:28525753, PubMed:33930289). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed:28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA): specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed:28525753, PubMed:29051200, PubMed:32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed:29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed:27872311).
Alternative names
METT10D, METTL16, RNA N(6)-adenosine-methyltransferase METTL16, Methyltransferase 10 domain-containing protein, Methyltransferase-like protein 16, U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase
Recommended products
Rabbit Polyclonal METTL16 antibody. Suitable for WB and reacts with Human, Mouse samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human METTL16 aa 350-450.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 99% Tris citrate/phosphate- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human METTL16 aa 350-450. The exact immunogen used to generate this antibody is proprietary information. Database link Q86W50
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
METTL16 or Methyltransferase-like protein 16 is a human enzyme known for catalyzing m6A methylation in RNA. The protein has a molecular mass of approximately 60 kDa. METTL16 is expressed in various tissues with higher levels in the liver kidney and brain. It also participates in the splicing and stabilization of RNA influencing gene regulation at the post-transcriptional level.
- Biological function summary
METTL16 influences RNA stability and processing. It acts as a component of a larger ribonucleoprotein complex that interacts with a variety of RNA substrates. This protein primarily targets U6 spliceosomal RNA and MAT2A RNA to modulate their function and degradation affecting regulatory feedback mechanisms in cells. By targeting specific RNA molecules METTL16 can modulate cellular response to various stimuli at the RNA level.
- Pathways
METTL16 plays an important role in RNA metabolism and gene expression regulation pathways. It cooperates with the S-adenosylmethionine (SAM) pathway which is vital for cellular methylation processes. METTL16 works alongside other m6A writers such as METTL3 and METTL14 to establish particular methylation marks on RNA influencing translation and stability which affects cell cycle regulation and differentiation.
- Associated diseases and disorders
METTL16 has implications in cancer and metabolic disorders. Dysregulation of its activity could lead to abnormal m6A methylation patterns contributing to tumorigenesis. Research has linked METTL16 to hepatocellular carcinoma where it interacts with other cancer-related proteins such as c-Myc affecting tumor growth and metabolism. Additionally its role in regulating MAT2A RNA associates it with metabolic anomalies implicating possible connections to diseases such as metabolic syndrome.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
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2 product images
Western blot - Anti-METTL16 antibody (ab186012)
All lanes: Western blot - Anti-METTL16 antibody (ab186012) at 0.1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: 293T whole cell lysate at 50 µg
Lane 3: Jurkat whole cell lysate at 50 µg
Lane 4: TCMK-1 whole cell lysate at 50 µg
Lane 5: NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 64 kDa
Exposure time: 3min
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-METTL16 antibody (ab186012)
METTL16 western blot using anti-METTL16 antibody ab186012. Publication image and figure legend from Jabs, S., Biton, A., et al., 2020, Nat Commun, PubMed 32165618.
ab186012 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab186012 please see the product overview.
Differential expression of Mettl16 and Mat2a in mouse cecum.a Western blot analysis of Mettl16 expression from ceca of CONV, GF, ex-GF, abx, vanco, Am, and Lp mice. Actin served as loading control. The membrane and thus the actin loading control for the blot displaying samples from ex-GF, Am, and Lp mice is identical to the western blot in Supplementary Fig. 4a. b Quantification using six different western blots; CONV n = 20, ex-GF n = 6; GF n = 13; abx n = 9; vanco n = 8; Am n = 9; Lp n = 9; Mettl16/Actin ratio was normalized to ratio in CONV mice in order to compare protein expression across multiple Western Blots. Ordinary one-way ANOVA was performed. *p-value < 0.05; p-values (compared to CONV): ex-GF: 0.9518; GF: 0.0255; abx: 0.0431; vanco: 0.1750; Am: 0.1783; Lp: 0.2728) (Holm–Sidak’s multiple comparisons test). c Representation of two m6A peaks (mean of read per million normalized coverage (RPM) in detected methylation peaks) from anti-m6A immunoprecipitates and input in the 3′UTR of the Mat2a transcript in cecum. The peaks designated a and b were visualized for the indicated mouse models using IGV; d Quantification of indicated Mat2a peaks (a, b from b) as -log2 normalized read counts. Ordinary one-way ANOVA for multiple comparisons was performed. CONV (n = 15), GF (n = 12); ex-GF (n = 4); abx (n = 9); vanco (n = 8); Am (n = 11), Lp (n = 3); two independent sequencing experiments; ap-values (all compared to CONV): ex-GF: 0.6458; GF: 0.0039; abx: 0.0572; vanco: 0.6458; Am: 0.6458; Lp: 0.4695; bp-values (all compared to CONV): ex-GF: 0.889955; GF: 0.000323; abx: 0.003604; vanco: 0.889955; Am: 0.00000000000028; Lp: 0.018424; e anti-m6A-IP and qRT for Mat2a transcript in CONV (n = 11), ex-GF(n = 5) and GF (n = 8) cecal RNA; IgG-IP (n = 4) served as control for unspecific binding; ordinary one-way ANOVA was performed; *p-value < 0.05; ***p-value < 0.005; p-values (compared to CONV): ex-GF: 0.6897; GF: 0.0127; IgG: 0.0029; f Western Blot analysis of Mat2a expression in CONV, GF, ex-GF, abx, vanco, Am, and Lp cecum. Actin served as loading control. Asterisk marks an unspecific band. g Quantification of Mat2a expression in CONV, GF, ex-GF, abx, vanco, Am, and Lp cecum. Quantification was performed using at least three different western blots for each condition; CONV n = 15, ex-GF n = 6; GF n = 12; abx n = 12; vanco n = 12; Am n = 9; Lp n = 9; Mat2a/Actin ratio was normalized to ratio in CONV mice in order to compare protein expression across multiple western blots; ordinary one-way ANOVA was performed. ***<0.0001, **p-value < 0.005 (Holm–Sidak’s multiple comparisons test); p-values (all compared to CONV): ex-GF: 0.157; GF: 0.00000019; abx: 0.0002; vanco: 0.0019; Am: 0.1111; Lp: 0.154; data are presented as mean values ±SEM throughout the figure; details for statistical analysis and original data for a, b, d–g are given in the source data file.
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