AB252420

Anti-METTL16 antibody [EPR23459-39]

RabMAb
Recombinant
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(10 Publications)

Rabbit Recombinant Monoclonal METTL16 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 10 publications.

View Alternative Names

METT10D, METTL16, RNA N(6)-adenosine-methyltransferase METTL16, Methyltransferase 10 domain-containing protein, Methyltransferase-like protein 16, U6 small nuclear RNA (adenine-(43)-N(6))-methyltransferase

2 Images

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Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (AB252420)

METTL16 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate with ab252420 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab252420 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate 10 ug

Lane 2 : ab252420 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab252420 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 69 seconds

The band below 50 kDa is caused by degradation as demonstrated by WB data.

All lanes:

Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (ab252420)

Predicted band size: 64 kDa

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Lab

Western blot - Anti-METTL16 antibody [EPR23459-39] (AB252420)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Lysates were made freshly and used in WB test immediately to minimize protein degradation (lanes 1-3). Lanes 4-6 are the lysates from same cell lines but have experienced freeze-thaw cycle.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 29051200, 31940410).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-METTL16 antibody [EPR23459-39] (ab252420) at 1/1000 dilution

Lanes 1 and 4:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 2 and 5:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lanes 3 and 6:

RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 64 kDa

Observed band size: 75 kDa

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Carrier free

Anti-METTL16 antibody [EPR23459-39] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23459-39

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL16 or Methyltransferase-like protein 16 is a human enzyme known for catalyzing m6A methylation in RNA. The protein has a molecular mass of approximately 60 kDa. METTL16 is expressed in various tissues with higher levels in the liver kidney and brain. It also participates in the splicing and stabilization of RNA influencing gene regulation at the post-transcriptional level.

Biological function summary

METTL16 influences RNA stability and processing. It acts as a component of a larger ribonucleoprotein complex that interacts with a variety of RNA substrates. This protein primarily targets U6 spliceosomal RNA and MAT2A RNA to modulate their function and degradation affecting regulatory feedback mechanisms in cells. By targeting specific RNA molecules METTL16 can modulate cellular response to various stimuli at the RNA level.

Pathways

METTL16 plays an important role in RNA metabolism and gene expression regulation pathways. It cooperates with the S-adenosylmethionine (SAM) pathway which is vital for cellular methylation processes. METTL16 works alongside other m6A writers such as METTL3 and METTL14 to establish particular methylation marks on RNA influencing translation and stability which affects cell cycle regulation and differentiation.

METTL16 has implications in cancer and metabolic disorders. Dysregulation of its activity could lead to abnormal m6A methylation patterns contributing to tumorigenesis. Research has linked METTL16 to hepatocellular carcinoma where it interacts with other cancer-related proteins such as c-Myc affecting tumor growth and metabolism. Additionally its role in regulating MAT2A RNA associates it with metabolic anomalies implicating possible connections to diseases such as metabolic syndrome.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

RNA N6-methyltransferase that methylates adenosine residues at the N(6) position of a subset of RNAs and is involved in S-adenosyl-L-methionine homeostasis by regulating expression of MAT2A transcripts (PubMed : 28525753, PubMed : 30197297, PubMed : 30197299, PubMed : 33428944, PubMed : 33930289). Able to N6-methylate a subset of mRNAs and U6 small nuclear RNAs (U6 snRNAs) (PubMed : 28525753). In contrast to the METTL3-METTL14 heterodimer, only able to methylate a limited number of RNAs : requires both a 5'UACAGAGAA-3' nonamer sequence and a specific RNA structure (PubMed : 28525753, PubMed : 30197297, PubMed : 30197299). Plays a key role in S-adenosyl-L-methionine homeostasis by mediating N6-methylation of MAT2A mRNAs, altering splicing of MAT2A transcripts : in presence of S-adenosyl-L-methionine, binds the 3'-UTR region of MAT2A mRNA and specifically N6-methylates the first hairpin of MAT2A mRNA, preventing recognition of their 3'-splice site by U2AF1/U2AF35, thereby inhibiting splicing and protein production of S-adenosylmethionine synthase (PubMed : 28525753, PubMed : 33930289). In S-adenosyl-L-methionine-limiting conditions, binds the 3'-UTR region of MAT2A mRNA but stalls due to the lack of a methyl donor, preventing N6-methylation and promoting expression of MAT2A (PubMed : 28525753). In addition to mRNAs, also able to mediate N6-methylation of U6 small nuclear RNA (U6 snRNA) : specifically N6-methylates adenine in position 43 of U6 snRNAs (PubMed : 28525753, PubMed : 29051200, PubMed : 32266935). Also able to bind various lncRNAs, such as 7SK snRNA (7SK RNA) or 7SL RNA (PubMed : 29051200). Specifically binds the 3'-end of the MALAT1 long non-coding RNA (PubMed : 27872311).

See full target information METTL16

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 21:4782-4797 PubMed40860196

2025

METTL16 Promotes Lipid Metabolic Reprogramming and Colorectal Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Li,Qian Luo,Minjie Lu,Chen Lu,Caihong Xu,Jie Ding,Tian Zhan,Jing Zhu,Mengsen Qian,Shuhui Lin,Lisha Chang,Juan Li,Keming Wang

Neuroreport 36:719-727 PubMed40736326

2025

Ubiquitin-specific peptidase 30-mediated deubiquitination of forkhead box O3 promotes the progression of subarachnoid hemorrhage by regulating cGAS/STING pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Guiqiang Zhang,Wenfeng Xu

Frontiers in aging neuroscience 17:1572976 PubMed40308894

2025

Effects of mA methylation of MAT2A mRNA regulated by METTL16 on learning and memory, hippocampal synaptic plasticity and Aβ in 5 × FAD mice.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Chen,Fangzhen Guo,Yan Zhao,Wei Liu,Bingyu Chen,Chang Wang,Lining Huang,Sufang Jiang,Xiaowei Ma,Huiling Ren,Sha Li,Huixian Cui

Cell division 20:9 PubMed40251670

2025

METTL16/IGF2BP2 axis enhances malignant progression and DDP resistance through up-regulating COL4A1 by mediating the m6A methylation modification of LAMA4 in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liming Cao,Wei Bi

Journal of hematology & oncology 17:78 PubMed39218945

2024

METTL16-SENP3-LTF axis confers ferroptosis resistance and facilitates tumorigenesis in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Jialin Wang,Mengxi Xiu,Jin Wang,Yong Gao,Yandong Li

PloS one 19:e0306043 PubMed39088431

2024

METTL16 participates in haemoglobin H disease through m6A modification.

Applications

Unspecified application

Species

Unspecified reactive species

Yuping Liao,Feng Zhang,Fang Yang,Shijin Huang,Sha Su,Xuemei Tan,Linlin Zhong,Lingjie Deng,Lihong Pang

Journal of experimental & clinical cancer research : CR 43:185 PubMed38965575

2024

A novel lncRNA LOC101928222 promotes colorectal cancer angiogenesis by stabilizing HMGCS2 mRNA and increasing cholesterol synthesis.

Applications

Unspecified application

Species

Unspecified reactive species

Lisha Chang,Jie Ding,Juan Pu,Jing Zhu,Xiang Zhou,Qian Luo,Jie Li,Mengsen Qian,Shuhui Lin,Juan Li,Keming Wang

The EMBO journal 43:1990-2014 PubMed38605226

2024

A Mettl16/mA/mybl2b/Igf2bp1 axis ensures cell cycle progression of embryonic hematopoietic stem and progenitor cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yunqiao Han,Kui Sun,Shanshan Yu,Yayun Qin,Zuxiao Zhang,Jiong Luo,Hualei Hu,Liyan Dai,Manman Cui,Chaolin Jiang,Fei Liu,Yuwen Huang,Pan Gao,Xiang Chen,Tianqing Xin,Xiang Ren,Xiaoyan Wu,Jieping Song,Qing Wang,Zhaohui Tang,Jianjun Chen,Haojian Zhang,Xianqin Zhang,Mugen Liu,Daji Luo

Aging 15:8444-8457 PubMed37647025

2023

mA methyltransferase METTL16 mediates immune evasion of colorectal cancer cells via epigenetically regulating PD-L1 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Ailei Wang,Yingjie Sun,Xince Wang,Zhaofei Yan,Dongsheng Wang,Li Zeng,Qingge Lu

Discover. Oncology 13:89 PubMed36114893

2022

METTL3 dual regulation of the stability of LINC00662 and VEGFA RNAs promotes colorectal cancer angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Guoying Zhang,Tianjun Wang,Zihui Huang,Yuanyuan Chen,Li Sun,Xia Xia,Fang He,Chenying Fan,Shukui Wang,Wanli Liu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-METTL16 antibody [EPR23459-39]

Immunoprecipitation - Anti-METTL16 antibody [EPR23459-39] (AB252420)

Western blot - Anti-METTL16 antibody [EPR23459-39] (AB252420)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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