Anti-METTL2 antibody [EPR26701-205]

• IP

Supplier Data

Immunoprecipitation - Anti-METTL2 antibody [EPR26701-205] (AB324877)

METTL2 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab324877 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab324877 at 1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 2:

ab324877 at 1/30 IP in Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab324877 in Neuro-2 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 44 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

Neuro-2a transfected with siRNA specifically targeting METTL2 whole cell lysate at 20 µg

Lane 3:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324877 was shown to bind specifically to METTL2. Target of interest was observed at 44 kDa in wild-type mouse tissues lysates (lanes 1, 3, 5, 7) with no signal observed at this size in METTL2 knockout tissues (lanes 2, 4, 6, 8).

The lysates were kindly provided by Dr Jiaqiang Zhou's lab, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Wild-type mouse liver tissue lysate at 20 µg

Lane 2:

METTL2 knockout mouse liver tissue lysate at 20 µg

Lane 3:

Wild-type mouse brain tissue lysate at 20 µg

Lane 4:

METTL2 knockout mouse brain tissue lysate at 20 µg

Lane 5:

Wild-type mouse kidney tissue lysate at 20 µg

Lane 6:

METTL2 knockout mouse kidney tissue lysate at 20 µg

Lane 7:

Wild-type mouse skeletal muscle tissue lysate at 20 µg

Lane 8:

METTL2 knockout mouse skeletal muscle tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-METTL2 antibody [EPR26701-205] (AB324877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot, ab324877 was shown to bind specifically to METTL2. Target of interest was observed at 44 kDa in wild-type mouse tissues lysates (lanes 1, 3, 5) with no signal observed at this size in METTL2 knockout tissues (lanes 2, 4, 6).

The lysates were kindly provided by Dr Jiaqiang Zhou's lab, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-METTL2 antibody [EPR26701-205] (ab324877) at 1/1000 dilution

Lane 1:

Wild-type mouse subcutaneous adipose tissue lysate at 20 µg

Lane 2:

METTL2 knockout mouse subcutaneous adipose tissue lysate at 20 µg

Lane 3:

Wild-type mouse white adipose tissue lysate at 20 µg

Lane 4:

METTL2 knockout mouse white adipose tissue lysate at 20 µg

Lane 5:

Wild-type mouse brown adipose tissue lysate at 20 µg

Lane 6:

METTL2 knockout mouse brown adipose tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 44 kDa,36 kDa

false

Exposure time: 180s