Rabbit Polyclonal METTL2B antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human METTL2B aa 1-200.
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Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human METTL2B aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P1Q9
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Target data
Function
S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA(Thr)(UGU) and tRNA(Arg)(CCU).
Alternative names
tRNA N(3)-cytidine methyltransferase METTL2B, Methyltransferase-like protein 2B, METTL2B
Recommended products
Rabbit Polyclonal METTL2B antibody. Suitable for WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human METTL2B aa 1-200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human METTL2B aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link Q6P1Q9
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
METTL2B also known as Methyltransferase-like protein 2B serves as a methyltransferase enzyme. It has a mass around 55 kDa. This protein mainly functions in the methylation of RNA molecules. METTL2B is expressed in various tissues with higher levels in organs like liver and brain. It specifically methylates some RNA nucleotides which can influence RNA stability and processing.
- Biological function summary
METTL2B exerts influence by acting on RNA substrates modifying their properties and affecting gene expression regulation. It becomes part of methylation complexes though the exact components of these complexes still require precise characterization. Through its enzymatic activity METTL2B plays a role in genomic stability and through these actions can impact cell development and differentiation.
- Pathways
METTL2B influences several key biological cascades. It participates in RNA processing pathways and has connections to cellular signaling pathways important for maintaining normal cell function. In RNA processing METTL2B's activity is linked with other RNA methylation proteins such as METTL3 and METTL14 forming pathways that ensure proper RNA maturation and stability.
- Associated diseases and disorders
METTL2B has associations with certain cancers and neurological disorders. Abnormal expression levels of METTL2B can contribute to the development of cancers like hepatocellular carcinoma due to altered methylation patterns. Furthermore its dysregulation is related to neurological disorders such as schizophrenia where it might interact with proteins like FMRP impacting synaptic function and neuronal communication.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-METTL2B antibody (ab101510)
10% SDS PAGE
All lanes: Western blot - Anti-METTL2B antibody (ab101510) at 1/1000 dilution
All lanes: IMR32 whole cell lysate at 30 µg
Predicted band size: 43 kDa
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Product protocols
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