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AB261735

Anti-METTL4 antibody

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(1 Publication)

Rabbit Polyclonal METTL4 antibody. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human METTL4 aa 1-150.

View Alternative Names

N(6)-adenine-specific methyltransferase METTL4, Methyltransferase-like protein 4, N(6)-adenine-specific DNA methyltransferase METTL4, snRNA (2'-O-methyladenosine-N(6)-)-methyltransferase METTL4, METTL4

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL4 antibody (AB261735)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL4 antibody (AB261735)

Formalin/PFA-fixed paraffin-embedded human heart muscle tissue labeling METTL4 using ab261735 at a 1/50 dilution.

Retrieval method : HIER pH6.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human METTL4 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

Q8N3J2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL4 also known as Methyltransferase-Like Protein 4 plays a role as an RNA methyltransferase. It is a protein with a mass of approximately 70 kDa. METTL4 is expressed in various tissues with higher levels observed in neural tissues and embryonic stem cells. The protein is involved in the addition of methyl groups to RNA molecules modulating their stability and function within the cell.
Biological function summary

The methylation activity associated with METTL4 affects RNA processing transport and degradation. METTL4 acts as part of the m6A methylation pathway but may work independently suggesting it might have unique methylation targets or mechanisms. Its role influences gene expression regulation impacting developmental and differentiation processes. METTL4 works with partners in multiprotein complexes which can enhance its methylation precision and activity.

Pathways

METTL4 is involved in epigenetic regulation pathways that impact gene expression. It is part of the broader m6A methylation modification pathway which includes METTL3 and METTL14 known for their impacts on mRNA stability and translation efficiency. METTL4's activity integrates into these pathways including potential interactions with the RNA demethylase ALKBH5 emphasizing its role in dynamic methylation processes.

METTL4 has been linked to neurodevelopmental disorders and certain cancers. Aberrations in its methylation activities may contribute to unregulated cell growth and differentiation associated with such diseases. METTL4's association with proteins like METTL3 and METTL14 within the m6A pathway suggests that disruptions in this network could influence pathogenic processes affecting cellular responses and disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA (PubMed : 31913360, PubMed : 32183942). Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA) : methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation (PubMed : 31913360). Internal m6A(m) methylation of snRNAs regulates RNA splicing (PubMed : 31913360). Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine) (PubMed : 32183942). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo (PubMed : 32203414). Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation : m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending (PubMed : 32183942). N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing (By similarity).
See full target information METTL4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:19813 PubMed40473694

2025

Automated tissue dissociation for the establishment of human intestinal organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Woo Jin Yang,Eva Blahusova,Komal Nayak,Róisín M Owens,Matthias Zilbauer
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Product promise

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