Rabbit Polyclonal METTL8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human METTL8 aa 1-100.
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Publications
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- International journal of oncology 60:2021Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmina Bolatkan,Ken Asada,Syuzo Kaneko,Kruthi Suvarna,Noriko Ikawa,Hidenori Machino,Masaaki Komatsu,Shuichiro Shiina,Ryuji HamamotoPubMed 34913069
- iScience 24:1032712021miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSijie Xie,Xiaoxue Jiang,Rushi Qin,Shuting Song,Yanan Lu,Liyan Wang,Yingjie Chen,Dongdong LuPubMed 34761190
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human METTL8 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H825
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr) (PubMed:34774131, PubMed:35017528). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain (PubMed:34774131, PubMed:35017528). N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite (PubMed:34774131, PubMed:35017528). May also mediate N(3)-methylcytidine modification of mRNAs (PubMed:28655767). The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo (PubMed:33313824, PubMed:34774131).
Alternative names
Methyltransferase-like protein 8, mRNA N(3)-methylcytidine methyltransferase METTL8, METTL8
Recommended products
Rabbit Polyclonal METTL8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human METTL8 aa 1-100.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human METTL8 aa 1-100. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H825
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
METTL8 also known as Methyltransferase-like protein 8 is an enzyme that catalyzes the methylation of RNA molecules. It belongs to the larger family of methyltransferases which are essential for various methylation processes. METTL8 has a molecular mass of approximately 67 kDa. The expression of METTL8 is observed in multiple tissues but it shows higher levels in the brain and kidneys indicating a potential role in the regulation of processes specific to these organs.
- Biological function summary
METTL8 participates in the regulation of RNA stability and processing. It operates as part of a complex that modulates RNA modifications which can affect gene expression patterns. The methylation activity of METTL8 influences RNA metabolism having potential implications on the stability of transcripts and the overall efficiency of translation.
- Pathways
METTL8 involvement in RNA processing pathways becomes evident. It plays a role within the RNA methylation pathway by interacting with other methyltransferases like METTL3. This interaction suggests that METTL8 might have a function in the N6-methyladenosine (m6A) methylation pathway that modifies the nucleobases within RNA molecules to regulate gene expression. The significance of these pathways involves the control of diverse cellular processes including development and response to cellular stress.
- Associated diseases and disorders
Abnormalities in METTL8 function have potential links to neurological disorders and certain cancers. In particular altered expression levels or mutations in METTL8 may contribute to neurodegenerative diseases correlating with aberrant RNA methylation patterns. Its role in cancer comes from the association with dysregulated methylation often seen in tumorigenesis. METTL3 a related protein also connects to these diseases highlighting the importance of the methylation network in maintaining cellular homeostasis and its disruption in disease states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-METTL8 antibody (ab122273)
All lanes: Western blot - Anti-METTL8 antibody (ab122273) at 0.4 µg/mL
All lanes: Human cell line HepG2
Predicted band size: 33 kDa
Immunocytochemistry/ Immunofluorescence - Anti-METTL8 antibody (ab122273)
Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab122273 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL8 antibody (ab122273)
ab1222273, at 1/15 dilution, staining METTL8 in Paraffin-embedded Human stomach tissue by
Immunohistochemistry.
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