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AB122273

Anti-METTL8 antibody

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(4 Publications)

Rabbit Polyclonal METTL8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human METTL8 aa 1-100.

View Alternative Names

Methyltransferase-like protein 8, mRNA N(3)-methylcytidine methyltransferase METTL8, METTL8

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-METTL8 antibody (AB122273)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-METTL8 antibody (AB122273)

Immunofluorescent staining of Human cell line A-431 shows positivity in cytoplasm. Recommended concentration of ab122273 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL8 antibody (AB122273)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-METTL8 antibody (AB122273)

ab1222273, at 1/15 dilution, staining METTL8 in Paraffin-embedded Human stomach tissue by Immunohistochemistry.

Western blot - Anti-METTL8 antibody (AB122273)
  • WB

Unknown

Western blot - Anti-METTL8 antibody (AB122273)

All lanes:

Western blot - Anti-METTL8 antibody (ab122273) at 0.4 µg/mL

All lanes:

Human cell line HepG2

Predicted band size: 33 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human METTL8 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9H825

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

METTL8 also known as Methyltransferase-like protein 8 is an enzyme that catalyzes the methylation of RNA molecules. It belongs to the larger family of methyltransferases which are essential for various methylation processes. METTL8 has a molecular mass of approximately 67 kDa. The expression of METTL8 is observed in multiple tissues but it shows higher levels in the brain and kidneys indicating a potential role in the regulation of processes specific to these organs.
Biological function summary

METTL8 participates in the regulation of RNA stability and processing. It operates as part of a complex that modulates RNA modifications which can affect gene expression patterns. The methylation activity of METTL8 influences RNA metabolism having potential implications on the stability of transcripts and the overall efficiency of translation.

Pathways

METTL8 involvement in RNA processing pathways becomes evident. It plays a role within the RNA methylation pathway by interacting with other methyltransferases like METTL3. This interaction suggests that METTL8 might have a function in the N6-methyladenosine (m6A) methylation pathway that modifies the nucleobases within RNA molecules to regulate gene expression. The significance of these pathways involves the control of diverse cellular processes including development and response to cellular stress.

Abnormalities in METTL8 function have potential links to neurological disorders and certain cancers. In particular altered expression levels or mutations in METTL8 may contribute to neurodegenerative diseases correlating with aberrant RNA methylation patterns. Its role in cancer comes from the association with dysregulated methylation often seen in tumorigenesis. METTL3 a related protein also connects to these diseases highlighting the importance of the methylation network in maintaining cellular homeostasis and its disruption in disease states.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mitochondrial S-adenosyl-L-methionine-dependent methyltransferase that mediates N(3)-methylcytidine modification of residue 32 of the tRNA anticodon loop of mitochondrial tRNA(Ser)(UCN) and tRNA(Thr) (PubMed : 34774131, PubMed : 35017528). N(3)-methylcytidine methylation modification regulates mitochondrial translation efficiency and is required for activity of the respiratory chain (PubMed : 34774131, PubMed : 35017528). N(3)-methylcytidine methylation of mitochondrial tRNA(Ser)(UCN) requires the formation of N(6)-dimethylallyladenosine(37) (i6A37) by TRIT1 as prerequisite (PubMed : 34774131, PubMed : 35017528). May also mediate N(3)-methylcytidine modification of mRNAs (PubMed : 28655767). The existence of N(3)-methylcytidine modification on mRNAs is however unclear, and additional evidences are required to confirm the role of the N(3)-methylcytidine-specific mRNA methyltransferase activity of METTL8 in vivo (PubMed : 33313824, PubMed : 34774131).
See full target information METTL8
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

International journal of biological sciences 20:1045-1063 PubMed38322121

2024

The homeoprotein HOXB2 limits triple-negative breast carcinogenesis via extracellular matrix remodeling.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Hoon Oh,Clara Yuri Kim,Da Som Jeong,Yu Cheon Kim,Myoung Hee Kim,Je-Yoel Cho

Cell stem cell 30:300-311.e11 PubMed36764294

2023

Epitranscriptomic regulation of cortical neurogenesis via Mettl8-dependent mitochondrial tRNA mC modification.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Zhang,Kijun Yoon,Daniel Y Zhang,Nam-Shik Kim,Guo-Li Ming,Hongjun Song

International journal of oncology 60: PubMed34913069

2021

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Amina Bolatkan,Ken Asada,Syuzo Kaneko,Kruthi Suvarna,Noriko Ikawa,Hidenori Machino,Masaaki Komatsu,Shuichiro Shiina,Ryuji Hamamoto

iScience 24:103271 PubMed34761190

2021

miR-1307 promotes hepatocarcinogenesis by CALR-OSTC-endoplasmic reticulum protein folding pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Sijie Xie,Xiaoxue Jiang,Rushi Qin,Shuting Song,Yanan Lu,Liyan Wang,Yingjie Chen,Dongdong Lu
View all publications
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