Anti-MEX3A antibody

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3A antibody (AB79046)

Immunohistochemical analysis of paraffin-embedded human testis tissue using anti-RKHD4 antibody (ab79046) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3A antibody (AB79046)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue using anti-RKHD4 antibody (ab79046) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3A antibody (AB79046)

Immunohistochemical analysis of paraffin-embedded Rat testis tissue using anti-RKHD4 antibody (ab79046) at 1 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

• WB

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Western blot - Anti-MEX3A antibody (AB79046)

1 h incubation at RT in 5% NFDM/TBST.

All lanes:

Western blot - Anti-MEX3A antibody (ab79046) at 2 µg/mL

Lane 1:

Daudi cell lysates at 15 µg

Lane 2:

Mouse spleen tissue lysates at 15 µg

Lane 3:

Rat thymus tissue lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution

Predicted band size: 54 kDa

false

• WB

CiteAb

Western blot - Anti-MEX3A antibody (AB79046)

MEX3A western blot using anti-MEX3A antibody ab79046. Publication image and figure legend from Wang, X., Shan, Y. Q., et al., 2020, Cancer Cell Int, PubMed 32140076.

ab79046 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab79046 please see the product overview.

MEX3A is highly expressed in PDA and the construction of MEX3A knockdown cell model. a Expression levels of MEX3A in PDA tumor tissues and adjacent normal skin tissues were detected by IHC staining. b Kaplan–Meier survival analysis MEX3A expression and overall survival of PDA. c MEX3A expression in HPDE6-C7, BxPc-3, PANC-1 and SW1990 cells was detected by qRT-PCR. d qRT-PCR was used to screen knockdown efficiency of MEX3A in shMEX3A-1, shMEX3A-2, and shMEX3A-3 groups. e Infection efficiency for PANC-1 and SW1990 cells was evaluated by expression of green fluorescent protein 72 h post-infection. f, g The specificity and validity of the lentivirus-mediated shRNA knockdown of MEX3A expression was verified by qRT-PCR (f) and western blot analysis (g). The data were presented as the mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001

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