AB243457

Anti-MEX3C antibody

Name: Anti-MEX3C antibody (ab243457) | Abcam
Brand: Abcam Limited
SKU: AB243457
Price: 630 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal MEX3C antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human MEX3C aa 450-550.

View Alternative Names

RKHD2, RNF194, BM-013, MEX3C, RNA-binding E3 ubiquitin-protein ligase MEX3C, RING finger and KH domain-containing protein 2, RING finger protein 194, RING-type E3 ubiquitin transferase MEX3C

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-MEX3C antibody (AB243457)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-MEX3C antibody (AB243457)

PFA-fixed, Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for MEX3C (green) using ab243457 at 4 μg/ml in ICC/IF. Nuclei are shown in blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3C antibody (AB243457)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3C antibody (AB243457)

Formalin-fixed, paraffin-embedded human stomach tissue stained for MEX3C using ab243457 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MEX3C aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

Q5U5Q3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The biological target MEX3C also known as RKHD3 or MEX-3 homolog C is a protein that functions mechanically as an RNA-binding protein. It has a mass of approximately 73 kilodaltons. MEX3C is expressed in various tissues including the brain skeletal muscle and kidney. It contains KH domains responsible for binding RNA enabling it to regulate the fate of specific mRNA molecules influencing mRNA stability and translation.

Biological function summary

MEX3C acts as a post-transcriptional regulator and is involved in mRNA decay and modulation. It does not appear to be a part of any large protein complexes but regulates the expression of a variety of cellular factors by binding target mRNAs. This control mechanism allows MEX3C to influence cell differentiation and development by affecting protein synthesis at the post-transcriptional level.

Pathways

MEX3C is implicated in the regulation of the Wnt signaling pathway and the mRNA surveillance pathway. It interacts with proteins involved in these pathways to modulate transcriptional outputs and cell signaling processes which are vital for normal embryonic development and cellular homeostasis. Proteins such as β-catenin and UPF1 may interact with MEX3C as part of these pathways aligning MEX3C actions with control of cell proliferation and gene expression.

MEX3C has connections to cancer particularly in pathways that influence tumor growth and progression. Additionally MEX3C may play a role in neurodegenerative diseases as it is involved in mRNA regulation within neurons. Disorders linked with MEX3C involve proteins like p53 which is associated with the regulation of cell cycle and apoptosis illustrating the potential impact of MEX3C on cellular outcomes that pertain to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E3 ubiquitin ligase responsible for the post-transcriptional regulation of common HLA-A allotypes. Binds to the 3' UTR of HLA-A2 mRNA, and regulates its levels by promoting mRNA decay. RNA binding is sufficient to prevent translation, but ubiquitin ligase activity is required for mRNA degradation.

See full target information MEX3C

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e18215 PubMed38509755

2024

CCDC157 is essential for sperm differentiation and shows oligoasthenoteratozoospermia-related mutations in men.

Applications

Unspecified application

Species

Unspecified reactive species

Huimei Zheng,Chenjia Gong,Jingping Li,Jiaru Hou,Xinhan Gong,Xinhai Zhu,Huan Deng,Haoyue Wu,Fengbin Zhang,Qinghua Shi,Jianteng Zhou,Baolu Shi,Xiaohang Yang,Yongmei Xi

Journal of translational medicine 22:216 PubMed38424632

2024

Ubiquitylation of RUNX3 by RNA-binding ubiquitin ligase MEX3C promotes tumorigenesis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zelai He,Huijun Zhang,Haibo Xiao,Xiangyu Zhang,Hongbo Xu,Ruifen Sun,Siwen Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MEX3C antibody

Immunocytochemistry/ Immunofluorescence - Anti-MEX3C antibody (AB243457)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MEX3C antibody (AB243457)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise