AB129075

Anti-MFF antibody [EPR7360]

20ul selling size
RabMAb
Recombinant
What is this?

(1 Review)

(23 Publications)

Rabbit Recombinant Monoclonal MFF antibody. Suitable for WB and reacts with Human samples. Cited in 23 publications.

View Alternative Names

C2orf33, AD030, AD033, GL004, MFF, Mitochondrial fission factor

2 Images

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Western blot - Anti-MFF antibody [EPR7360] (AB129075)

All lanes:

Western blot - Anti-MFF antibody [EPR7360] (ab129075) at 1/1000 dilution

Lane 1:

Fetal muscle lysates at 10 µg

Lane 2:

Fetal heart lysates at 10 µg

Predicted band size: 38 kDa

Observed band size: 34 kDa

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OI-RD Scanning - Anti-MFF antibody [EPR7360] (AB129075)

OI-RD Scanning

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OI-RD Scanning - Anti-MFF antibody [EPR7360] (AB129075)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-MFF antibody [EPR7360] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7360

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondria fission factor (MFF) also known as C2orf33 or mitochondrial fission factor plays a significant role in the control of mitochondrial fission. MFF has a molecular weight of approximately 29 kDa and is expressed abundantly in tissues with high energy demands including the heart brain and muscles. By directly interacting with the dynamin-related protein 1 (Drp1) MFF facilitates the Drp1 recruitment to the mitochondrial outer membrane which is a critical step in the mitochondrial division process.

Biological function summary

The MFF protein promotes the division of mitochondria which is vital for maintaining the health and function of cells. MFF is a part of the mitochondrial fission complex a group of proteins responsible for regulating mitochondrial morphology. It plays an important role in cellular processes such as energy production apoptosis and cellular response to stress by modulating mitochondrial dynamics.

Pathways

MFF interacts with other proteins to regulate mitochondrial fission which is an integral part of the mitochondrial dynamics pathways. It participates prominently in pathways controlling energy metabolism and apoptosis. MFF works closely with proteins like Drp1 and has connections to pathways involving fission and fusion processes that maintain mitochondrial homeostasis and adapt to metabolic needs.

Disruptions in MFF function are associated with conditions like neurodegenerative diseases and metabolic disorders. Impaired mitochondrial fission can contribute to diseases such as Alzheimer's where abnormal mitochondrial dynamics play a role in disease progression. MFF's connection with Drp1 also implicates it in metabolic disorders where energy production and mitochondrial function are disrupted indicating its involvement in maintaining cellular energy balance and neuronal health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays a role in mitochondrial and peroxisomal fission (PubMed : 18353969, PubMed : 23530241, PubMed : 24196833). Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface (PubMed : 23530241). May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles (By similarity).

See full target information MFF

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Publications (23)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40862738

2025

Highly Oligomeric DRP1 Strategic Positioning at Mitochondria-Sarcoplasmic Reticulum Contacts in Adult Murine Heart Through ACTIN Anchoring.

Applications

Unspecified application

Species

Unspecified reactive species

Celia Fernandez-Sanz,Sergio De la Fuente,Zuzana Nichtova,Marilen Federico,Stephane Duvezin-Caubet,Sebastian Lanvermann,Hui-Ying Tsai,Yanguo Xin,Gyorgy Csordas,Wang Wang,Arnaud Mourier,Shey-Shing Sheu

BMC cancer 25:412 PubMed40050778

2025

Impact of celastrol on mitochondrial dynamics and proliferation in glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Liang,Wenying Lv,Gang Cheng,Mou Gao,Junzhao Sun,Ning Liu,Hanbo Zhang,Baorui Guo,Jiayu Liu,Yanteng Li,Shengqiang Xie,Jiangting Wang,Junru Hei,Jianning Zhang

BMC cardiovascular disorders 25:77 PubMed39901081

2025

HACE1 protects against myocardial ischemia-reperfusion injury via inhibition of mitochondrial fission in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Bang-Xia Liu,Juan Zheng,Zhan-Wei Tang,Lei Gao,Meng Wang,Ying Sun,Chen Chen,Heng-Chen Yao

iScience 27:110280 PubMed39055921

2024

Pim-1 kinase protects the liver from ischemia reperfusion injury by regulating dynamics-related protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Yan-Dong Sun,Qing-Guo Xu,De-Shu Dai,Shu-Xian Wang,Xin-Qiang Li,Shang-Heng Shi,Peng Jiang,Yan Jin,Xin Wang,Yong Zhang,Feng Wang,Peng Liu,Bing-Liang Zhang,Tian-Xiang Li,Chuan-Shen Xu,Bin Wu,Jin-Zhen Cai

Heliyon 10:e29807 PubMed38737244

2024

Dihydroquercetin regulates HIF-1α/AKT/NR2B signalling to improve impaired brain function in rats with metabolic syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Fu,PeiPei Yuan,Mengnan Zeng,Qi Zhang,Ying Hou,Liyuan Gao,Yaxin Wei,Yajuan Zheng,Weisheng Feng,Xiaoke Zheng

Cell death & disease 14:827 PubMed38092752

2023

Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Zhang,Yong Zhong,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Bo Wu,Zhongwei Lv

Cellular & molecular biology letters 28:57 PubMed37480044

2023

Mechanical force induces mitophagy-mediated anaerobic oxidation in periodontal ligament stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zijie Zhang,Shuyue Cui,Yajing Fu,Jixiao Wang,Jiani Liu,Fulan Wei

The British journal of nutrition 130:1473-1486 PubMed36815302

2023

Intermittent fasting promotes adipocyte mitochondrial fusion through Sirt3-mediated deacetylation of Mdh2.

Applications

Unspecified application

Species

Unspecified reactive species

Yizhou Li,Juntong Liang,Xin Tian,Qi Chen,Longbo Zhu,Han Wang,Zunhai Liu,Xulei Dai,Chenqi Bian,Chao Sun

eLife 11: PubMed36409067

2022

Collateral deletion of the mitochondrial AAA+ ATPase ATAD1 sensitizes cancer cells to proteasome dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob M Winter,Heidi L Fresenius,Corey N Cunningham,Peng Wei,Heather R Keys,Jordan Berg,Alex Bott,Tarun Yadav,Jeremy Ryan,Deepika Sirohi,Sheryl R Tripp,Paige Barta,Neeraj Agarwal,Anthony Letai,David M Sabatini,Matthew L Wohlever,Jared Rutter

Acta biochimica et biophysica Sinica 54:301-310 PubMed35538029

2022

Mitochondrial fission factor promotes cisplatin resistancein hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Li,Quanlin Wu,Fujun Ma,Xinxin Zhang,Lei Cai,Xuekang Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MFF antibody [EPR7360]

Western blot - Anti-MFF antibody [EPR7360] (AB129075)

OI-RD Scanning - Anti-MFF antibody [EPR7360] (AB129075)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (23)

Product promise