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AB248282

Anti-MFF antibody [EPR7360] - BSA and Azide free

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Rabbit Recombinant Monoclonal MFF antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

C2orf33, AD030, AD033, GL004, MFF, Mitochondrial fission factor

2 Images
Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)
  • WB

Unknown

Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)

This data was developed using ab129075, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MFF antibody [EPR7360] (<a href='/en-us/products/primary-antibodies/mff-antibody-epr7360-ab129075'>ab129075</a>) at 1/1000 dilution

Lane 1:

Fetal muscle lysates at 10 µg

Lane 2:

Fetal heart lysates at 10 µg

Predicted band size: 38 kDa

Observed band size: 34 kDa

false

OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR7360

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Primary Antibodies

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Primary Antibodies

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Secondary Antibodies

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Reactivity data

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Product details

ab248282 is the carrier-free version of ab129075.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The mitochondria fission factor (MFF) also known as C2orf33 or mitochondrial fission factor plays a significant role in the control of mitochondrial fission. MFF has a molecular weight of approximately 29 kDa and is expressed abundantly in tissues with high energy demands including the heart brain and muscles. By directly interacting with the dynamin-related protein 1 (Drp1) MFF facilitates the Drp1 recruitment to the mitochondrial outer membrane which is a critical step in the mitochondrial division process.
Biological function summary

The MFF protein promotes the division of mitochondria which is vital for maintaining the health and function of cells. MFF is a part of the mitochondrial fission complex a group of proteins responsible for regulating mitochondrial morphology. It plays an important role in cellular processes such as energy production apoptosis and cellular response to stress by modulating mitochondrial dynamics.

Pathways

MFF interacts with other proteins to regulate mitochondrial fission which is an integral part of the mitochondrial dynamics pathways. It participates prominently in pathways controlling energy metabolism and apoptosis. MFF works closely with proteins like Drp1 and has connections to pathways involving fission and fusion processes that maintain mitochondrial homeostasis and adapt to metabolic needs.

Disruptions in MFF function are associated with conditions like neurodegenerative diseases and metabolic disorders. Impaired mitochondrial fission can contribute to diseases such as Alzheimer's where abnormal mitochondrial dynamics play a role in disease progression. MFF's connection with Drp1 also implicates it in metabolic disorders where energy production and mitochondrial function are disrupted indicating its involvement in maintaining cellular energy balance and neuronal health.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in mitochondrial and peroxisomal fission (PubMed : 18353969, PubMed : 23530241, PubMed : 24196833). Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface (PubMed : 23530241). May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles (By similarity).
See full target information MFF
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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