Rabbit Recombinant Monoclonal MFF antibody. Carrier free. Suitable for WB and reacts with Human samples.
Images
![Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282), expandable thumbnail](https://content.abcam.com/products/images/mff-antibody-epr7360-bsa-and-azide-free-ab248282--western-blot-img123868.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)")
![OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282), expandable thumbnail](https://content.abcam.com/products/images/mff-antibody-epr7360-bsa-and-azide-free-ab248282--oi-rd-scanning-img95199.jpg/_jcr_content/renditions/webp-475.webp "OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (AB248282)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | |
|---|---|---|---|
| Human | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Predicted | Not recommended |
| Rat | Not recommended | Predicted | Not recommended |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Plays a role in mitochondrial and peroxisomal fission (PubMed:18353969, PubMed:23530241, PubMed:24196833). Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface (PubMed:23530241). May be involved in regulation of synaptic vesicle membrane dynamics by recruitment of DNM1L to clathrin-containing vesicles (By similarity).
Alternative names
C2orf33, AD030, AD033, GL004, MFF, Mitochondrial fission factor
Recommended products
Rabbit Recombinant Monoclonal MFF antibody. Carrier free. Suitable for WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR7360
- Purification technique
- Affinity purification Protein A
- Dissociation constant
- 6.1 x 10-11 M
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab248282 is the carrier-free version of Anti-MFF antibody [EPR7360] ab129075.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The mitochondria fission factor (MFF) also known as C2orf33 or mitochondrial fission factor plays a significant role in the control of mitochondrial fission. MFF has a molecular weight of approximately 29 kDa and is expressed abundantly in tissues with high energy demands including the heart brain and muscles. By directly interacting with the dynamin-related protein 1 (Drp1) MFF facilitates the Drp1 recruitment to the mitochondrial outer membrane which is a critical step in the mitochondrial division process.
- Biological function summary
The MFF protein promotes the division of mitochondria which is vital for maintaining the health and function of cells. MFF is a part of the mitochondrial fission complex a group of proteins responsible for regulating mitochondrial morphology. It plays an important role in cellular processes such as energy production apoptosis and cellular response to stress by modulating mitochondrial dynamics.
- Pathways
MFF interacts with other proteins to regulate mitochondrial fission which is an integral part of the mitochondrial dynamics pathways. It participates prominently in pathways controlling energy metabolism and apoptosis. MFF works closely with proteins like Drp1 and has connections to pathways involving fission and fusion processes that maintain mitochondrial homeostasis and adapt to metabolic needs.
- Associated diseases and disorders
Disruptions in MFF function are associated with conditions like neurodegenerative diseases and metabolic disorders. Impaired mitochondrial fission can contribute to diseases such as Alzheimer's where abnormal mitochondrial dynamics play a role in disease progression. MFF's connection with Drp1 also implicates it in metabolic disorders where energy production and mitochondrial function are disrupted indicating its involvement in maintaining cellular energy balance and neuronal health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282), expandable thumbnail](https://content.abcam.com/products/images/mff-antibody-epr7360-bsa-and-azide-free-ab248282--western-blot-img123868.jpg "Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282)")
Western blot - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282)
This data was developed using Anti-MFF antibody [EPR7360] ab129075, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-MFF antibody [EPR7360] (Anti-MFF antibody [EPR7360] ab129075) at 1/1000 dilution
Lane 1: Fetal muscle lysates at 10 µg
Lane 2: Fetal heart lysates at 10 µg
Predicted band size: 38 kDa
Observed band size: 34 kDa
![OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282), expandable thumbnail](https://content.abcam.com/products/images/mff-antibody-epr7360-bsa-and-azide-free-ab248282--oi-rd-scanning-img95199.jpg "OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282)")
OI-RD Scanning - Anti-MFF antibody [EPR7360] - BSA and Azide free (ab248282)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.
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