JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB249929

Anti-MFRP antibody [EPR11616] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal MFRP antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Membrane frizzled-related protein, Membrane-type frizzled-related protein, MFRP

1 Images
Western blot - Anti-MFRP antibody [EPR11616] - BSA and Azide free (AB249929)
  • WB

Supplier Data

Western blot - Anti-MFRP antibody [EPR11616] - BSA and Azide free (AB249929)

This data was developed using ab176574, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-MFRP antibody [EPR11616] (<a href='/en-us/products/primary-antibodies/mfrp-antibody-epr11616-ab176574'>ab176574</a>) at 1/1000 dilution

All lanes:

Human fetal brain tissue lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 62 kDa

true

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11616

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Primary Antibodies

AB184702

Anti-Rb (phospho S780) antibody [EPR17624]

20ul selling size|RabMAb|Recombinant|Trial Size

Immunoprecipitation - Anti-Rb (phospho S780) antibody [EPR17624] (AB184702)

  • 0
  • 0
Primary Antibodies

AB174280

Anti-TGN46 antibody [EPR12676] - Golgi Marker

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] - Golgi Marker (AB174280)

  • 0
  • 0
Primary Antibodies

AB40854

Anti-Smad3 antibody [EP568Y]

20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb

Immunocytochemistry/ Immunofluorescence - Anti-Smad3 antibody [EP568Y] (AB40854)

  • 5
  • 10
Primary Antibodies

AB249303

Anti-UPB1 antibody [EPR9132] - BSA and Azide free

RabMAb|Recombinant

Immunoprecipitation - Anti-UPB1 antibody [EPR9132] - BSA and Azide free (AB249303)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab249929 is the carrier-free version of ab176574.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Membrane Frizzled-Related Protein commonly known as MFRP is a protein encoded by the MFRP gene in humans. MFRP has a mass of approximately 62 kDa and functions at the cellular level as part of the control system for eye growth and visual acuity. This protein is mainly expressed in the retina particularly in the retinal pigment epithelium and the ciliary body. The presence of MFRP is significant for eye development and maintaining visual capabilities.
Biological function summary

MFRP acts to regulate the length of eye axial growth which is important in focusing light onto the retina. MFRP interacts with other components of the visual pathway and is believed to form a complex with CTRP5 another retinal protein to impact the structural and functional integrity of the eyes. This complex contributes to the stability of the retina and prevents excessive eye elongation that could lead to refractive issues.

Pathways

MFRP participates in the visual perception pathway and plays a regulatory role in the phototransduction pathway. It interacts with proteins related to these pathways such as G-protein coupled receptors which are essential for transmitting light signals from the retina to the brain. MFRP may influence the signaling mechanisms that govern gap junctions thereby affecting cellular communication and maintenance of retinal tissues.

Mutations or malfunctions in MFRP have strong associations with retinal dystrophies such as nanophthalmos and retinitis pigmentosa. These conditions lead to progressive vision loss and in some cases complete blindness due to the degeneration of photoreceptor cells in the retina. Studies have shown that MFRP dysfunction influences interactions with proteins like Protocadherin 21 thereby exacerbating retinal degenerative processes and impacting visual health.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

May play a role in eye development.
See full target information MFRP
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 7:357 PubMed34785642

2021

UBE2S promotes cell chemoresistance through PTEN-AKT signaling in hepatocellular carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Gui,Sicai Zhang,Yongzi Xu,Hongwei Zhang,Ying Zhu,Lianbao Kong
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com