AB307690

Anti-MFSD2A/NLS1 antibody [EPR24503-101]

20ul selling size
RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal MFSD2A/NLS1 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MFSD2, NLS1, HMFN0656, PP9177, UNQ300/PRO341, MFSD2A, Sodium-dependent lysophosphatidylcholine symporter 1, Sodium-dependent LPC symporter 1, Major facilitator superfamily domain-containing protein 2A, HsMFSD2A, MFSD2a

1 Images

Supplier Data

Western blot - Anti-MFSD2A/NLS1 antibody [EPR24503-101] (AB307690)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 32117064).

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution, anti-BSA antibody (ab192603) loading control staining at 1/10000 dilution.

Low expression : placenta (PMID : 32117064).

Samples are non-boiled as boiling may cause protein aggregation.

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-MFSD2A/NLS1 antibody [EPR24503-101] (ab307690) at 1/1000 dilution

Lane 1:

Human serum lysate at 20 µg

Lane 2:

Human placenta tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 60 kDa

Observed band size: 50 kDa,100 kDa

true

Exposure time: 15s

Carrier free

Anti-MFSD2A/NLS1 antibody [EPR24503-101] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24503-101

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

MFSD2A also known as Major Facilitator Superfamily Domain-Containing Protein 2A functions as a transporter protein. It efficiently facilitates transport of specific lipids across cellular membranes. This protein has a mass of approximately 56 kDa and is mainly expressed in the blood-brain barrier and endothelial cells. MFSD2A's expression in these areas plays an important role in transport through the protective barrier in the brain.

Biological function summary

MFSD2A is important in mediating uptake of omega-3 fatty acids such as docosahexaenoic acid (DHA) into the brain. It achieves this by ensuring proper distribution of lipids necessary for maintaining brain health and function. While not known to be a part of a larger complex MFSD2A works closely with lipid molecules and other membrane transporters to regulate lipid homeostasis within the brain.

Pathways

MFSD2A is involved in the lipid metabolic pathway and the neurovascular signaling pathway. Its activity significantly affects how lipids are transported and utilized within these systems. It interacts functionally with proteins like ABC transporters which also contribute to lipid processing and transport. These pathways highlight the importance of MFSD2A in lipid utilization and brain metabolism.

MFSD2A is closely linked to neurological conditions such as microcephaly and certain neurodevelopmental disorders. Its malfunction or altered expression can disrupt the supply of essential lipids to the brain leading to developmental abnormalities. The protein's role connects to other transporters like FABP5 hinting at a network of lipid transport mechanisms that when altered contribute to these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Sodium-dependent lysophosphatidylcholine (LPC) symporter, which plays an essential role for blood-brain barrier formation and function (PubMed : 24828040, PubMed : 32572202, PubMed : 34135507). Specifically expressed in endothelium of the blood-brain barrier of micro-vessels and transports LPC into the brain (By similarity). Transport of LPC is essential because it constitutes the major mechanism by which docosahexaenoic acid (DHA), an omega-3 fatty acid that is essential for normal brain growth and cognitive function, enters the brain (PubMed : 26005868, PubMed : 34135507). Transports LPC carrying long-chain fatty acids such LPC oleate and LPC palmitate with a minimum acyl chain length of 14 carbons (By similarity). Does not transport docosahexaenoic acid in unesterified fatty acid (By similarity). Specifically required for blood-brain barrier formation and function, probably by mediating lipid transport (By similarity). Not required for central nervous system vascular morphogenesis (By similarity). Acts as a transporter for tunicamycin, an inhibitor of asparagine-linked glycosylation (PubMed : 21677192). In placenta, acts as a receptor for ERVFRD-1/syncytin-2 and is required for trophoblast fusion (PubMed : 18988732, PubMed : 23177091).

See full target information MFSD2A

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:926 PubMed39715736

2024

Sphk1/S1P pathway promotes blood-brain barrier breakdown after intracerebral hemorrhage through inducing Nlrp3-mediated endothelial cell pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Mengzhao Feng,Yuan An,Qi Qin,Iat-Hang Fong,Kaiyuan Zhang,Fang Wang,Dengpan Song,Mengyuan Li,Min Yu,Chi-Tai Yeh,Junlei Chang,Fuyou Guo

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-MFSD2A/NLS1 antibody [EPR24503-101]

Western blot - Anti-MFSD2A/NLS1 antibody [EPR24503-101] (AB307690)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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