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AB254816

Anti-MFSD8 antibody

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(1 Publication)

Rabbit Polyclonal MFSD8 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human MFSD8 aa 1-50.

View Alternative Names

CLN7, MFSD8, Major facilitator superfamily domain-containing protein 8, Ceroid-lipofuscinosis neuronal protein 7

5 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)

Paraffin-embedded human fallopian tube tissue stained for MFSD8 using ab254816 at 1/50 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-MFSD8 antibody (AB254816)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-MFSD8 antibody (AB254816)

PFA fixed, Triton X-100 permeabilized U-251 MG (Human brain glioma cell line) cells labeling MFSD8 using ab254816 at 4µg/ml (green) in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)

Paraffin-embedded human kidney tissue stained for MFSD8 using ab254816 at 1/50 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MFSD8 antibody (AB254816)

Paraffin-embedded human testis tissue stained for MFSD8 using ab254816 at 1/50 dilution in immunohistochemical analysis.

Western blot - Anti-MFSD8 antibody (AB254816)
  • WB

Supplier Data

Western blot - Anti-MFSD8 antibody (AB254816)

All lanes:

Western blot - Anti-MFSD8 antibody (ab254816) at 0.4 µg/mL

Lane 1:

Vector only transfected HEK-293T cell lysate

Lane 2:

MFSD8 overexpressed HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Predicted band size: 58 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human MFSD8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q8NHS3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MFSD8 protein also known as Major Facilitator Superfamily Domain containing protein 8 is an integral membrane protein involved in lysosomal function. It has a molecular mass of approximately 63 kDa. MFSD8 is expressed in a variety of tissues with high expression levels in the brain and retina. This suggests its importance in neurological and visual functions. The protein is part of the major facilitator superfamily which is known for its role in transporting small solutes across cell membranes.
Biological function summary

MFSD8 functions in lysosomal maintenance and intracellular transport processes. It plays an important role in transporting molecules within cells and maintaining lysosomal homeostasis. MFSD8 associates with other lysosomal proteins and components to execute its functions efficiently. By stabilizing lysosomal membranes it aids in the proper degradation of cellular waste highlighting its role within the cellular recycling system.

Pathways

MFSD8 participates in cellular nutrient sensing and waste management pathways namely the lysosomal degradative pathway and mTOR signaling pathway. The protein works alongside other important lysosomal proteins such as LAMP1 and LAMP2 to ensure effective lysosome function. Through its role in the mTOR signaling pathway MFSD8 influences cellular metabolism and growth processes linking it to nutrient availability and cellular energy status.

Abnormalities in MFSD8 have connections to neuronal ceroid lipofuscinosis a group of neurodegenerative disorders particularly variant CLN7. Mutations in the MFSD8 gene lead to malfunction in lysosomal autophagy resulting in cell toxicity and death. These mutations often correlate with protein instability and defective lysosomal activity. Additionally MFSD8 associations with retinal dystrophy highlight its significant role in visual health and disease progression impacting proteins involved in retinal maintenance.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Outward-rectifying chloride channel involved in endolysosomal chloride homeostasis, membrane fusion and function. Conducts chloride currents up to hundreds of picoamperes. Regulates lysosomal calcium content by reducing the lysosomal membrane potential, thereby activating TRPML1 channel and further release of lysosomal calcium ions. Regulates the pH in endolysosomal compartments and may contribute to progressive acidification from endosome to lysosome. Permeable to other halides such as iodide and fluoride ions.
See full target information MFSD8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncogenesis 11:19 PubMed35459265

2022

TCRP1 activated by mutant p53 promotes NSCLC proliferation via inhibiting FOXO3a.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Liu,Xiaoting Jia,Kai Luo,Xiangzhou Chen,Zhijie Zhang,Danyang Chen,Yixue Gu,Zhimin He,Guopei Zheng
View all publications
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Product promise

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