Rabbit Polyclonal MG53 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM72 aa 50-300.
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Publications
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- Nature structural & molecular biology 30:1695-17062023Structure and activation of the RING E3 ubiquitin ligase TRIM72 on the membrane.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSi Hoon Park,Juhyun Han,Byung-Cheon Jeong,Ju Han Song,Se Hwan Jang,Hyeongseop Jeong,Bong Heon Kim,Young-Gyu Ko,Zee-Yong Park,Kyung Eun Lee,Jaekyung Hyun,Hyun Kyu SongPubMed 37770719
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human TRIM72 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZMU5
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Rat | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Muscle-specific E3 ubiquitin-protein ligase that plays a central role in cell membrane repair by nucleating the assembly of the repair machinery at injury sites (PubMed:36944613). Its ubiquitination activity is mediated by E2 ubiquitin-conjugating enzymes UBE2D1, UBE2D2 and UBE2D3 (By similarity). Acts as a sensor of oxidation: upon membrane damage, entry of extracellular oxidative environment results in disulfide bond formation and homooligomerization at the injury site (By similarity). This oligomerization acts as a nucleation site for recruitment of TRIM72-containing vesicles to the injury site, leading to membrane patch formation (By similarity). Probably acts upstream of the Ca(2+)-dependent membrane resealing process (By similarity). Required for transport of DYSF to sites of cell injury during repair patch formation (By similarity). Regulates membrane budding and exocytosis (By similarity). May be involved in the regulation of the mobility of KCNB1-containing endocytic vesicles (By similarity).
Alternative names
MG53, TRIM72, Tripartite motif-containing protein 72, Mitsugumin-53, Mg53
Recommended products
Rabbit Polyclonal MG53 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human TRIM72 aa 50-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human TRIM72 aa 50-300. The exact immunogen used to generate this antibody is proprietary information. Database link Q6ZMU5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
MG53 also known as TRIM72 is a protein that plays an important role in membrane repair. This protein has a molecular mass of approximately 72 kDa and is classified as a member of the tripartite motif-containing (TRIM) protein family. MG53 is expressed highly in striated muscles including the cardiac and skeletal muscles. Its mechanical action involves sensing membrane disruptions and facilitating the repair process by recruiting vesicles to the site of injury.
- Biological function summary
MG53 acts as a critical factor in maintaining cellular membrane integrity. It forms part of a complex network where it interacts with other proteins to orchestrate the membrane repair process. MG53 functions as a scaffold bringing together various repair components and coordinating their activity to mend damaged membranes. This repair process is essential for muscle cells which frequently experience mechanical stress.
- Pathways
MG53 integrates into cellular repair pathways and is pivotal in the response to membrane damage. It interacts with important pathways like the IRS1/PI3K/AKT signaling pathway where it modulates cellular responses to stress and injury. MG53 also associates with other proteins such as caveolin-3 assisting in stabilizing the membrane during injury response.
- Associated diseases and disorders
MG53 has relevance to conditions like muscular dystrophy and cardiomyopathy. Mutations or dysregulation of MG53 can lead to inadequate membrane repair contributing to muscle weakness and cardiac dysfunction. In muscular disorders MG53 interacts with dysferlin another repair protein to support membrane stabilization and disruptions in this interaction exacerbate disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-MG53 antibody (ab154238)
12% SDS PAGE
All lanes: Western blot - Anti-MG53 antibody (ab154238) at 1/1000 dilution
All lanes: HCT116 whole cell lysate at 30 µg
Predicted band size: 53 kDa
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