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AB254622

Anti-MGA antibody

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Rabbit Polyclonal MGA antibody. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human MGA aa 2050 to C-terminus.

View Alternative Names

KIAA0518, MAD5, MGA, MAX gene-associated protein, MAX dimerization protein 5

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)

Paraffin-embedded human testis tissue stained for MGA using ab254622 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)

Paraffin-embedded human skeletal muscle tissue stained for MGA using ab254622 at 1/1000 dilution in immunohistochemical analysis. No positivity, as expected.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)

Paraffin-embedded human endometrium tissue stained for MGA using ab254622 at 1/1000 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGA antibody (AB254622)

Paraffin-embedded human uterine tissue stained for MGA using ab254622 at 1/1000 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human MGA aa 2050 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8IWI9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The MGA protein also known as MAX gene-associated protein plays a role in gene regulation by acting as a transcriptional repressor. It has an approximate mass of 256 kDa and primarily locates itself within the nucleus. MGA exhibits a broad expression pattern found in tissues such as the brain liver and heart. The protein's structure includes several domains that allow it to interact with other proteins and DNA to modulate gene expression.
Biological function summary

MGA influences the regulation of developmental processes and cellular growth. It forms part of a complex with the MAX protein essential for its function in repressing specific gene sets. This ability allows MGA to modulate cellular differentiation and development processes. By controlling these gene sets it ensures proper cellular function and maintains homeostasis within the organism.

Pathways

MGA integrates itself into the MYC-MAX-MAD network a critical regulatory system for cell proliferation. It offers a balancing effect by counteracting the actions of MYC a protein known for promoting cell cycle progression and proliferation. MGA influences the transcription machinery through this pathway to ensure controlled cell growth. Additionally MGA-related pathways intersect with those involving the E2F family further influencing cellular proliferation and apoptosis.

MGA has significant connections to cancer particularly due to its role in the MYC-MAX-MAD pathway. Dysregulation of MGA can lead to unchecked cell growth contributing to tumor development. In particular defects in MGA have been observed in lung and other carcinomas. Its relationship with MYC protein highlights its importance as MYC overexpression often couples with reduced MGA function in these cancers. Besides cancer MGA also associates with certain neurodevelopmental disorders where its role in developmental processes becomes compromised disrupting normal neurological development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as a dual-specificity transcription factor, regulating the expression of both MAX-network and T-box family target genes. Functions as a repressor or an activator. Binds to 5'-AATTTCACACCTAGGTGTGAAATT-3' core sequence and seems to regulate MYC-MAX target genes. Suppresses transcriptional activation by MYC and inhibits MYC-dependent cell transformation. Function activated by heterodimerization with MAX. This heterodimerization serves the dual function of both generating an E-box-binding heterodimer and simultaneously blocking interaction of a corepressor (By similarity).
See full target information MGA
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Product promise

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